{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699994080089984.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/aem.72.5.3702-3709.2006"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/AEM.72.5.3702-3709.2006"}},{"identifier":{"@type":"PMID","@value":"16672520"}}],"dc:title":[{"@value":"Isolation and Characterization of a New\n            <i>Clostridium</i>\n            sp. That Performs Effective Cellulosic Waste Digestion in a Thermophilic Methanogenic Bioreactor"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            A methanogenic bioreactor that utilized wastepaper was developed and operated at 55°C. Microbial community structure analysis showed the presence of a group of clostridia that specifically occurred during the period of high fermentation efficiency. To isolate the effective cellulose digester, the sludge that exhibited high fermentation efficiency was inoculated into a synthetic medium that contained cellulose powder as the sole carbon source and was successively cultivated. A comprehensive 16S rRNA gene sequencing study revealed that the enriched culture contained various clostridia that had diverse phylogenetic positions. The microorganisms were further enriched by successive cultivation with filter paper as the substrate, as well as the bait carrier. A resultant isolate, strain EBR45 (=\n            <jats:italic>Clostridium</jats:italic>\n            sp. strain NBRC101661), was a new member of the order\n            <jats:italic>Clostridiales</jats:italic>\n            phylogenetically and physiologically related to\n            <jats:italic>Clostridium thermocellum</jats:italic>\n            and\n            <jats:italic>Clostridium straminisolvens</jats:italic>\n            . Specific PCR-based monitoring demonstrated that strain EBR45 specifically occurred during the high fermentation efficiency period in the original methanogenic sludge. Strain EBR45 effectively digested office paper in its pure cultivation system with a synthetic medium.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699994080089987","@type":"Researcher","foaf:name":[{"@value":"Hatsumi Shiratori"}],"jpcoar:affiliationName":[{"@value":"Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994080089986","@type":"Researcher","foaf:name":[{"@value":"Hironori Ikeno"}],"jpcoar:affiliationName":[{"@value":"Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994080089988","@type":"Researcher","foaf:name":[{"@value":"Shohei Ayame"}],"jpcoar:affiliationName":[{"@value":"Ebara Research Co., Ltd., 4-2-1 Honfujisawa, Fujisawa 251-8502, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994080089984","@type":"Researcher","foaf:name":[{"@value":"Naoaki Kataoka"}],"jpcoar:affiliationName":[{"@value":"Ebara Research Co., Ltd., 4-2-1 Honfujisawa, Fujisawa 251-8502, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994080089985","@type":"Researcher","foaf:name":[{"@value":"Akiko Miya"}],"jpcoar:affiliationName":[{"@value":"Ebara Research Co., Ltd., 4-2-1 Honfujisawa, Fujisawa 251-8502, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994080089989","@type":"Researcher","foaf:name":[{"@value":"Kuniaki Hosono"}],"jpcoar:affiliationName":[{"@value":"Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994080089991","@type":"Researcher","foaf:name":[{"@value":"Teruhiko Beppu"}],"jpcoar:affiliationName":[{"@value":"Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994080089990","@type":"Researcher","foaf:name":[{"@value":"Kenji Ueda"}],"jpcoar:affiliationName":[{"@value":"Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00992240"},{"@type":"EISSN","@value":"10985336"}],"prism:publicationName":[{"@value":"Applied and Environmental Microbiology"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"2006-05","prism:volume":"72","prism:number":"5","prism:startingPage":"3702","prism:endingPage":"3709"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/AEM.72.5.3702-3709.2006"}],"createdAt":"2006-05-04","modifiedAt":"2022-02-23","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Clostridium","dc:title":"Clostridium"},{"@id":"https://cir.nii.ac.jp/all?q=DNA,%20Bacterial","dc:title":"DNA, Bacterial"},{"@id":"https://cir.nii.ac.jp/all?q=Paper","dc:title":"Paper"},{"@id":"https://cir.nii.ac.jp/all?q=Hot%20Temperature","dc:title":"Hot Temperature"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Sequence%20Data","dc:title":"Molecular Sequence Data"},{"@id":"https://cir.nii.ac.jp/all?q=Sequence%20Analysis,%20DNA","dc:title":"Sequence Analysis, DNA"},{"@id":"https://cir.nii.ac.jp/all?q=Refuse%20Disposal","dc:title":"Refuse Disposal"},{"@id":"https://cir.nii.ac.jp/all?q=Bioreactors","dc:title":"Bioreactors"},{"@id":"https://cir.nii.ac.jp/all?q=RNA,%20Ribosomal,%2016S","dc:title":"RNA, Ribosomal, 16S"},{"@id":"https://cir.nii.ac.jp/all?q=Cellulose","dc:title":"Cellulose"},{"@id":"https://cir.nii.ac.jp/all?q=Methane","dc:title":"Methane"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004235769713664","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Isolation of digested sludge-assimilating fungal strains and their potential applications"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283691836451072","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Enzymatic diversity of the Clostridium thermocellum cellulosome is crucial for the degradation of crystalline cellulose and plant biomass"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285710955440128","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Role and Function of LitR, an Adenosyl B\n            <sub>12</sub>\n            -Bound Light-Sensitive Regulator of Bacillus megaterium QM B1551, in Regulation of Carotenoid Production"}]},{"@id":"https://cir.nii.ac.jp/crid/1360847869095846400","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Acceleration of cellulose degradation and shift of product via methanogenic co-culture of a cellulolytic bacterium with a hydrogenotrophic methanogen"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848656433270272","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Comparative Biochemical Analysis of Cellulosomes Isolated from Clostridium clariflavum DSM 19732 and Clostridium thermocellum ATCC 27405 Grown on Plant Biomass"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679123870464","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Unique diversity content of a thermophilic anaerobic microbial consortium that utilizes propionate in a synthetic medium"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/aem.72.5.3702-3709.2006"},{"@type":"OPENAIRE","@value":"doi_dedup___::7f3639d664de1b2dbea58583d0ab1c4c"},{"@type":"CROSSREF","@value":"10.1111/jam.12266_references_DOI_46bkKLvK4oD5iB8sPhvPbCMMo49"},{"@type":"CROSSREF","@value":"10.2323/jgam.53.363_references_DOI_46bkKLvK4oD5iB8sPhvPbCMMo49"},{"@type":"CROSSREF","@value":"10.1038/srep35709_references_DOI_46bkKLvK4oD5iB8sPhvPbCMMo49"},{"@type":"CROSSREF","@value":"10.1128/jb.02528-14_references_DOI_46bkKLvK4oD5iB8sPhvPbCMMo49"},{"@type":"CROSSREF","@value":"10.1007/s12010-018-2864-6_references_DOI_46bkKLvK4oD5iB8sPhvPbCMMo49"},{"@type":"CROSSREF","@value":"10.1016/j.jbiosc.2012.05.002_references_DOI_46bkKLvK4oD5iB8sPhvPbCMMo49"}]}