{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699994097943040.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/jvi.01118-06"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/JVI.01118-06"}}],"dc:title":[{"@value":"Proteolytic Activation of Influenza Viruses by Serine Proteases TMPRSS2 and HAT from Human Airway Epithelium"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>Host cell proteases that cleave the hemagglutinin (HA) of influenza viruses in the human respiratory tract are still not identified. Here we cloned two human type II transmembrane serine proteases with known airway localization, TMPRSS2 and HAT, into mammalian expression vector. Cotransfection of mammalian cells with plasmids encoding HA and either protease resulted in HA cleavage in situ. Transient expression of either protease in MDCK cells enabled multicycle replication of influenza viruses in these cells in the absence of exogenous trypsin. These data suggest that TMPRSS2 and HAT are candidates for proteolytic activation of influenza viruses in vivo.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699994097943168","@type":"Researcher","foaf:name":[{"@value":"Eva Böttcher"}],"jpcoar:affiliationName":[{"@value":"Institute of Virology, Philipps University, 35043 Marburg, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994097943044","@type":"Researcher","foaf:name":[{"@value":"Tatyana Matrosovich"}],"jpcoar:affiliationName":[{"@value":"Institute of Virology, Philipps University, 35043 Marburg, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994097943042","@type":"Researcher","foaf:name":[{"@value":"Michaela Beyerle"}],"jpcoar:affiliationName":[{"@value":"Institute of Virology, Philipps University, 35043 Marburg, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994097943043","@type":"Researcher","foaf:name":[{"@value":"Hans-Dieter Klenk"}],"jpcoar:affiliationName":[{"@value":"Institute of Virology, Philipps University, 35043 Marburg, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994097943040","@type":"Researcher","foaf:name":[{"@value":"Wolfgang Garten"}],"jpcoar:affiliationName":[{"@value":"Institute of Virology, Philipps University, 35043 Marburg, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994097943041","@type":"Researcher","foaf:name":[{"@value":"Mikhail Matrosovich"}],"jpcoar:affiliationName":[{"@value":"Institute of Virology, Philipps University, 35043 Marburg, Germany"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0022538X"},{"@type":"EISSN","@value":"10985514"}],"prism:publicationName":[{"@value":"Journal of Virology"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"2006-10","prism:volume":"80","prism:number":"19","prism:startingPage":"9896","prism:endingPage":"9898"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/JVI.01118-06"}],"createdAt":"2006-09-14","modifiedAt":"2022-03-05","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050568772217138688","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Enterokinase Enhances Influenza A Virus Infection by Activating Trypsinogen in Human Cell Lines"}]},{"@id":"https://cir.nii.ac.jp/crid/1050584642173767168","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The respective roles of TMPRSS2 and cathepsins for SARS-CoV-2 infection in human respiratory organoids"}]},{"@id":"https://cir.nii.ac.jp/crid/1050866680462678784","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Generation of a Porcine Cell Line Stably Expressing Pig TMPRSS2 for Efficient Isolation of Swine Influenza Virus"}]},{"@id":"https://cir.nii.ac.jp/crid/1050867133849402368","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Crystal structure of inhibitor-bound human MSPL that can activate high pathogenic avian influenza"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004231273419008","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"IL-1β is a key cytokine that induces trypsin upregulation in the influenza virus–cytokine–trypsin cycle"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004234612891776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A unique nanoparticulate TLR9 agonist enables a HA split vaccine to confer FcγR-mediated protection against heterologous lethal influenza virus infection"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004235985131648","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A Mutant H3N2 Influenza Virus Uses an Alternative Activation Mechanism in TMPRSS2 Knockout Mice by Loss of an Oligosaccharide in the Hemagglutinin Stalk Region"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004235988657536","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The Host Protease TMPRSS2 Plays a Major Role in\n            <i>In Vivo</i>\n            Replication of Emerging H7N9 and Seasonal Influenza Viruses"}]},{"@id":"https://cir.nii.ac.jp/crid/1360009142849908864","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Existence of SARS-CoV-2 Entry Molecules in the Oral Cavity"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283693444008064","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Novel Type II Transmembrane Serine Proteases, MSPL and TMPRSS13, Proteolytically Activate Membrane Fusion Activity of the Hemagglutinin of Highly Pathogenic Avian Influenza Viruses and Induce Their Multicycle Replication"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285709462296448","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Unique Infectious Strategy of H5N1 Avian Influenza Virus Is Governed by the Acid-Destabilized Property of Hemagglutinin"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285709909851136","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Insight into structural diversity of influenza virus haemagglutinin"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285710963989760","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"TMPRSS2 Contributes to Virus Spread and Immunopathology in the Airways of Murine Models after Coronavirus Infection"}]},{"@id":"https://cir.nii.ac.jp/crid/1360294643741380480","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"TMPRSS2 Activates Hemagglutinin-Esterase Glycoprotein of Influenza C Virus"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565166089755776","@type":"Article","resourceType":"学術雑誌論文(journal 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article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Structure-Guided Creation of an Anti-HA Stalk Antibody F11 Derivative That Neutralizes Both F11-Sensitive and -Resistant Influenza A(H1N1)pdm09 Viruses"}]},{"@id":"https://cir.nii.ac.jp/crid/1360580230622521472","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"TMPRSS2 gene polymorphism common in East Asians confers decreased COVID-19 susceptibility"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846639485473664","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Transmembrane serine protease TMPRSS2 activates hepatitis C virus infection"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848656925231872","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Direct visualization of avian influenza H5N1 hemagglutinin precursor and its conformational change by high-speed atomic force microscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848656926447616","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Role of host cellular proteases in the pathogenesis of influenza and influenza-induced multiple organ failure"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848658466208000","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"TMPRSS2 Independency for Haemagglutinin Cleavage In Vivo Differentiates Influenza B Virus from Influenza A Virus"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848658911857920","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Avian Influenza Virus Infection of Immortalized Human Respiratory Epithelial Cells Depends upon a Delicate Balance 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Coronaviruses"},{"@language":"ja","@value":"プロテアーゼ依存的なコロナウイルス細胞侵入"}]},{"@id":"https://cir.nii.ac.jp/crid/1390289455275590144","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Basis of coronavirus infection, and SARS-CoV-2"},{"@language":"ja","@value":"国立感染症研究所ウイルス第3部"},{"@value":"コロナウイルス感染の基礎とSARS-CoV-2"},{"@language":"ja-Kana","@value":"コロナウイルス カンセン ノ キソ ト SARS-CoV-2"}]},{"@id":"https://cir.nii.ac.jp/crid/1390567172573538304","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Protease-dependent virus tropism and pathogenicity: The role for TMPRSS2"},{"@language":"ja","@value":"プロテアーゼ依存性ウイルス病原性発現機構とTMPRSS2"},{"@language":"ja-Kana","@value":"プロテアーゼ イソンセイ ウイルス ビョウゲンセイ ハツゲン キコウ ト TMPRSS2"}]},{"@id":"https://cir.nii.ac.jp/crid/1390567172574846976","@type":"Article","resourceType":"学術雑誌論文(journal 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