{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699994454604416.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1373/jalm.2016.022913"}},{"identifier":{"@type":"URI","@value":"https://academic.oup.com/jalm/article-pdf/2/2/186/42353456/2_2_2_186.pdf"}},{"identifier":{"@type":"PMID","@value":"32630971"}}],"dc:title":[{"@value":"Cholesterol Uptake Capacity: A New Measure of HDL Functionality for Coronary Risk Assessment"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n               <jats:sec>\n                  <jats:title>Background</jats:title>\n                  <jats:p>Recent studies have shown that the cholesterol efflux capacity of HDL is a better predictor of cardiovascular disease (CVD) than HDL cholesterol. However, the standard procedures used for measuring cholesterol efflux capacity involve radioisotope-labeled cholesterol and cultured macrophages. Thus, a simpler method to measure HDL functionality is needed for clinical application.</jats:p>\n               </jats:sec>\n               <jats:sec>\n                  <jats:title>Methods</jats:title>\n                  <jats:p>We established a cell-free assay system to evaluate the capacity of HDL to accept additional cholesterol, which we named cholesterol “uptake capacity,” using fluorescently labeled cholesterol and an anti-apolipoprotein A1 antibody. We quantified cholesterol uptake capacity of apolipoprotein B (apoB)-depleted serum samples from patients with coronary artery disease who had previously undergone revascularization.</jats:p>\n               </jats:sec>\n               <jats:sec>\n                  <jats:title>Results</jats:title>\n                  <jats:p>This assay system exhibited high reproducibility (CV &lt;10%) and a short processing time (&lt;6 h). The myeloperoxidase-mediated oxidation of apoB-depleted serum impaired cholesterol uptake capacity. Cholesterol uptake capacity correlated significantly with cholesterol efflux capacity (r  2 = 0.47, n = 30). Furthermore, cholesterol uptake capacity correlated inversely with the requirement for revascularization because of recurrence of coronary lesions in patients with optimal control of LDL cholesterol (P &lt; 0.01, n = 156). A multivariate analysis adjusted for traditional coronary risk factors showed that only cholesterol uptake capacity remained significant (odds ratio, 0.48; 95% CI, 0.29–0.80; P = 0.0048).</jats:p>\n               </jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions</jats:title>\n                  <jats:p>Cholesterol uptake capacity assay evaluates the functionality of HDL in a sensitive and high-throughput manner without using radioisotope label and cells. This assay system could be used for the assessment of CVD risk in the clinical settings.</jats:p>\n               </jats:sec>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699994454604416","@type":"Researcher","foaf:name":[{"@value":"Amane Harada"}],"jpcoar:affiliationName":[{"@value":"Central Research Laboratories, Sysmex Corporation, Kobe, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994454604420","@type":"Researcher","foaf:name":[{"@value":"Ryuji Toh"}],"jpcoar:affiliationName":[{"@value":"Division of Evidence-Based Laboratory Medicine and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994454604417","@type":"Researcher","foaf:name":[{"@value":"Katsuhiro Murakami"}],"jpcoar:affiliationName":[{"@value":"Central Research Laboratories, Sysmex Corporation, Kobe, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994454604423","@type":"Researcher","foaf:name":[{"@value":"Maria Kiriyama"}],"jpcoar:affiliationName":[{"@value":"Central Research Laboratories, Sysmex Corporation, Kobe, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994454604547","@type":"Researcher","foaf:name":[{"@value":"Keiko Yoshikawa"}],"jpcoar:affiliationName":[{"@value":"Central Research Laboratories, Sysmex Corporation, Kobe, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994454604544","@type":"Researcher","foaf:name":[{"@value":"Keiko Miwa"}],"jpcoar:affiliationName":[{"@value":"Central Research Laboratories, Sysmex Corporation, Kobe, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994454604546","@type":"Researcher","foaf:name":[{"@value":"Takuya Kubo"}],"jpcoar:affiliationName":[{"@value":"Central Research Laboratories, Sysmex Corporation, Kobe, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994454604422","@type":"Researcher","foaf:name":[{"@value":"Yasuhiro Irino"}],"jpcoar:affiliationName":[{"@value":"Division of Evidence-Based Laboratory Medicine and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994454604421","@type":"Researcher","foaf:name":[{"@value":"Kenta Mori"}],"jpcoar:affiliationName":[{"@value":"Division of Cardiovascular Medicine, Kobe University Graduate School of Medicine, Kobe, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994454604424","@type":"Researcher","foaf:name":[{"@value":"Nobuaki Tanaka"}],"jpcoar:affiliationName":[{"@value":"Division of Cardiovascular Medicine, Kobe University Graduate School of Medicine, Kobe, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994454604418","@type":"Researcher","foaf:name":[{"@value":"Kunihiro Nishimura"}],"jpcoar:affiliationName":[{"@value":"Department of Preventive Medicine and Epidemiologic Informatics, Office of Evidence-Based Medicine and Risk Analysis, National Cerebral and Cardiovascular Center, Suita, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994454604419","@type":"Researcher","foaf:name":[{"@value":"Tatsuro Ishida"}],"jpcoar:affiliationName":[{"@value":"Division of Cardiovascular Medicine, Kobe University Graduate School of Medicine, Kobe, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994454604545","@type":"Researcher","foaf:name":[{"@value":"Ken-ichi Hirata"}],"jpcoar:affiliationName":[{"@value":"Division of Evidence-Based Laboratory Medicine and"},{"@value":"Division of Cardiovascular Medicine, Kobe University Graduate School of Medicine, Kobe, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"25769456"},{"@type":"EISSN","@value":"24757241"}],"prism:publicationName":[{"@value":"The Journal of Applied Laboratory Medicine"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2017-09-01","prism:volume":"2","prism:number":"2","prism:startingPage":"186","prism:endingPage":"200"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model"],"url":[{"@id":"https://academic.oup.com/jalm/article-pdf/2/2/186/42353456/2_2_2_186.pdf"}],"createdAt":"2018-07-23","modifiedAt":"2022-02-01","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050012570392525312","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Impaired Cholesterol-Uptake Capacity of HDL Might Promote Target-Lesion Revascularization by Inducing Neoatherosclerosis After Stent Implantation"},{"@value":"Impaired Cholesterol‐Uptake Capacity of HDL Might Promote Target‐Lesion Revascularization by Inducing Neoatherosclerosis After Stent 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