{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699994771862272.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/jvi.74.6.2567-2583.2000"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/JVI.74.6.2567-2583.2000"}},{"identifier":{"@type":"NAID","@value":"80011578012"}}],"dc:title":[{"@value":"Efficient Gene Transfer into Human CD34\n            <sup>+</sup>\n            Cells by a Retargeted Adenovirus Vector"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Efficient infection with adenovirus (Ad) vectors based on serotype 5 (Ad5) requires the presence of coxsackievirus-adenovirus receptors (CAR) and α\n            <jats:sub>v</jats:sub>\n            integrins on cells. The paucity of these cellular receptors is thought to be a limiting factor for Ad gene transfer into hematopoietic stem cells. In a systematic approach, we screened different Ad serotypes for interaction with noncycling human CD34\n            <jats:sup>+</jats:sup>\n            cells and K562 cells on the level of virus attachment, internalization, and replication. From these studies, serotype 35 emerged as the variant with the highest tropism for CD34\n            <jats:sup>+</jats:sup>\n            cells. A chimeric vector (Ad5GFP/F35) was generated which contained the short-shafted Ad35 fiber incorporated into an Ad5 capsid. This substitution was sufficient to transplant all infection properties from Ad35 to the chimeric vector. The retargeted, chimeric vector attached to a receptor different from CAR and entered cells by an α\n            <jats:sub>v</jats:sub>\n            integrin-independent pathway. In transduction studies, Ad5GFP/F35 expressed green fluorescent protein (GFP) in 54% of CD34\n            <jats:sup>+</jats:sup>\n            cells. In comparison, the standard Ad5GFP vector conferred GFP expression to only 25% of CD34\n            <jats:sup>+</jats:sup>\n            cells. Importantly, Ad5GFP transduction, but not Ad5GFP/F35, was restricted to a specific subset of CD34\n            <jats:sup>+</jats:sup>\n            cells expressing α\n            <jats:sub>v</jats:sub>\n            integrins. The actual transduction efficiency was even higher than 50% because Ad5GFP/F35 viral genomes were found in GFP-negative CD34\n            <jats:sup>+</jats:sup>\n            cell fractions, indicating that the cytomegalovirus promoter used for transgene expression was not active in all transduced cells. The chimeric vector allowed for gene transfer into a broader spectrum of CD34\n            <jats:sup>+</jats:sup>\n            cells, including subsets with potential stem cell capacity. Fifty-five percent of CD34\n            <jats:sup>+</jats:sup>\n            c-Kit\n            <jats:sup>+</jats:sup>\n            cells expressed GFP after infection with Ad5GFP/F35, whereas only 13% of CD34\n            <jats:sup>+</jats:sup>\n            c-Kit\n            <jats:sup>+</jats:sup>\n            cells were GFP positive after infection with Ad5GFP. These findings represent the basis for studies aimed toward stable gene transfer into hematopoietic stem cells.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699994771862273","@type":"Researcher","foaf:name":[{"@value":"Dmitry M. Shayakhmetov"}],"jpcoar:affiliationName":[{"@value":"<!--label omitted: 1-->Division of Medical Genetics1 and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994771862272","@type":"Researcher","foaf:name":[{"@value":"Thalia Papayannopoulou"}],"jpcoar:affiliationName":[{"@value":"<!--label omitted: 2-->Division of Hematology,2 Department of Medicine, University of Washington, Seattle, Washington 98195"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994771862274","@type":"Researcher","foaf:name":[{"@value":"George Stamatoyannopoulos"}],"jpcoar:affiliationName":[{"@value":"<!--label omitted: 1-->Division of Medical Genetics1 and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994771862275","@type":"Researcher","foaf:name":[{"@value":"André Lieber"}],"jpcoar:affiliationName":[{"@value":"<!--label omitted: 1-->Division of Medical Genetics1 and"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0022538X"},{"@type":"EISSN","@value":"10985514"}],"prism:publicationName":[{"@value":"Journal of Virology"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"2000-03-15","prism:volume":"74","prism:number":"6","prism:startingPage":"2567","prism:endingPage":"2583"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/JVI.74.6.2567-2583.2000"}],"createdAt":"2002-07-27","modifiedAt":"2022-03-05","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050022089736941952","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Solid-state Thermal Transformation of Methylammonium Monomolybdate to Polyoxomolybdates and their Applications as Staining Reagents for Transmission Electron Microscopy Observations of Viruses"}]},{"@id":"https://cir.nii.ac.jp/crid/1050866514666817792","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Structure Transformation of Methylammonium Polyoxomolybdates via In-Solution Acidification and Solid-State Heating from Methylammonium Monomolybdate and Application as Negative Staining Reagents for Coronavirus Observation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283693969162368","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Cytotoxic effects of replication-competent adenoviruses on human esophageal carcinoma are enhanced by forced p53 expression"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285708375954944","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Combinatory cytotoxic effects produced by E1B-55kDa-deleted adenoviruses and chemotherapeutic agents are dependent on the agents in esophageal carcinoma"}]},{"@id":"https://cir.nii.ac.jp/crid/1360290088388576640","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Adenovirus vector-based vaccine for infectious diseases"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567183352648832","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Cytotoxicity of adenoviruses expressing the wild-type p53 gene to esophageal carcinoma cells is linked with the CAR expression level and indirectly with the endogenous p53 status"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848658334537984","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"BTK gene targeting by homologous recombination using a helper-dependent adenovirus/adeno-associated virus hybrid vector"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848661281475072","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Evaluation of Transduction Properties of an Adenovirus Vector in Neonatal Mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1360849946804672000","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A survivin-responsive, conditionally replicating adenovirus induces potent cytocidal effects in adult T-cell leukemia/lymphoma"}]},{"@id":"https://cir.nii.ac.jp/crid/1360869856044932352","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Clinical Application of Adenovirus (AdV): A Comprehensive Review"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204275165696","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Development of improved adenovirus vectors and transduction into neural cells"},{"@language":"ja","@value":"改良型アデノウイルスベクターの開発と神経系細胞への遺伝子導入"},{"@value":"実験技術 改良型アデノウイルスベクターの開発と神経系細胞への遺伝子導入"},{"@language":"ja-Kana","@value":"ジッケン ギジュツ カイリョウガタ アデノウイルスベクター ノ カイハツ ト シンケイケイ サイボウ エ ノ イデンシ ドウニュウ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206128266496","@type":"Article","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"35型アデノウイルスを基本骨格とした新規アデノウイルスベクターの開発"},{"@language":"en","@value":"Development of a Replication-incompetent Adenovirus Vector Derived from Subgroup B Adenovirus Serotype 35"},{"@language":"ja-Kana","@value":"35ガタ アデノウイルス オ キホン コッカク ト シタ シンキ アデノウイルス ベクター ノ カイハツ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679600908672","@type":"Article","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Development and Evaluation of a Novel Gene Delivery Vehicle Composed of Adenovirus Serotype 35"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680155923968","@type":"Article","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Targeting of adenoviral vector to immature hematopoietic cells mediated by a soluble CAR fusion protein"},{"@language":"ja","@value":"可溶性ＣＡＲ融合蛋白質を介したアデノウイルスベクターの細胞ターゲティング"}]},{"@id":"https://cir.nii.ac.jp/crid/1570291225910496768","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Expression of the coxsackievirus and adenovirus receptor in musculoskeletal tumors and mesenchymal tissues : efficacy of adenoviral gene therapy for osteosarcoma"}]},{"@id":"https://cir.nii.ac.jp/crid/1571698599601605632","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Hematopoietic Stem Cell Gene Therapy"}]},{"@id":"https://cir.nii.ac.jp/crid/1572824499409740928","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Targeted Integration of Foreign DNA Into a Defined Locus on Chromosome 19 in K562 Cells Using AAV-Derived 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