{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699994772916736.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1099/mic.0.26535-0"}},{"identifier":{"@type":"URI","@value":"https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.26535-0?crawler=true"}}],"dc:title":[{"@value":"The surface (S-) layer is a virulence factor of Bacteroides forsythus"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>\n                  <jats:italic>Bacteroides forsythus</jats:italic> has emerged as a crucial periodontal pathogen with possible implications for systemic disease. The aim of this study was to isolate the S-layer from <jats:italic>B. forsythus</jats:italic> and examine its virulence potential as a part of efforts to characterize virulence factors of <jats:italic>B. forsythus</jats:italic>. The role of the S-layer in the haemagglutinating and adherent/invasive activities was evaluated. It was observed that the S-layer alone was able to mediate haemagglutination. In adherent and invasive studies, transmission electron microscopy clearly revealed that <jats:italic>B. forsythus</jats:italic> cells were able to attach to and invade KB cells, showing the formation of a microvillus-like extension around adherent and intracellular bacteria. The quantitative analysis showed that five different <jats:italic>B. forsythus</jats:italic> strains exhibited attachment (1·9–2·3 %) and invasion (0·4–0·7 %) capabilities. It was also observed through antibody inhibition assays that adherent/invasive activities of <jats:italic>B. forsythus</jats:italic> are mediated by the S-layer. Furthermore, an <jats:italic>in vivo</jats:italic> immunization study adopting a murine abscess model was used to prove that the S-layer is involved in the infectious process of abscess formation. While mice immunized with purified S-layer and <jats:italic>B. forsythus</jats:italic> whole cells did not develop any abscesses when challenged with viable <jats:italic>B. forsythus</jats:italic> cells, unimmunized mice developed abscesses. Collectively, the data obtained from these studies indicate that the S-layer of <jats:italic>B. forsythus</jats:italic> is a virulence factor.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004239211319822","@type":"Researcher","foaf:name":[{"@value":"M. Sabet"}],"jpcoar:affiliationName":[{"@value":"Department of Biomedical Sciences, University of Maryland, Baltimore, Dental School, 666 W. Baltimore Street, Baltimore, MD 21201, USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994772916738","@type":"Researcher","foaf:name":[{"@value":"S.-W. Lee"}],"jpcoar:affiliationName":[{"@value":"Department of Periodontics, University of Maryland, Baltimore, Dental School, 666 W. Baltimore Street, Baltimore, MD 21201, USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994772916737","@type":"Researcher","foaf:name":[{"@value":"R. K. Nauman"}],"jpcoar:affiliationName":[{"@value":"Department of Biomedical Sciences, University of Maryland, Baltimore, Dental School, 666 W. Baltimore Street, Baltimore, MD 21201, USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994772916739","@type":"Researcher","foaf:name":[{"@value":"T. Sims"}],"jpcoar:affiliationName":[{"@value":"Oral Health Research Center, University of Washington, Seattle, WA, USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994772916736","@type":"Researcher","foaf:name":[{"@value":"H.-S. Um"}],"jpcoar:affiliationName":[{"@value":"Department of Periodontics, University of Maryland, Baltimore, Dental School, 666 W. Baltimore Street, Baltimore, MD 21201, USA"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13500872"},{"@type":"EISSN","@value":"14652080"}],"prism:publicationName":[{"@value":"Microbiology"}],"dc:publisher":[{"@value":"Microbiology Society"}],"prism:publicationDate":"2003-12-01","prism:volume":"149","prism:number":"12","prism:startingPage":"3617","prism:endingPage":"3627"},"reviewed":"false","url":[{"@id":"https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.26535-0?crawler=true"}],"createdAt":"2003-12-08","modifiedAt":"2020-11-06","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050001338456661632","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"nergy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surfaceEnergy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surface layer proteins from Tannerella forsythensis using microwave or autoclave heating with citraconic anhydride."},{"@language":"en","@value":"Energy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surface layer proteins from Tannerella forsythensis using microwave or autoclave heating with citraconic anhydride."}]},{"@id":"https://cir.nii.ac.jp/crid/1050287297252051840","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Lack of a surface layer in Tannerella forsythia mutants deficient in the type IX secretion system"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004230172775552","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Energy dispersive spectroscopy‐scanning transmission electron microscope observations of free radical production in human polymorphonuclear leukocytes phagocytosing non‐opsonized <i>Tannerella forsythia</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004235974355200","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The Surface Layer of Tannerella forsythia Contributes to Serum Resistance and Oral Bacterial Coaggregation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285706959166464","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Lysine-specific proteolytic activity responsible for forsythia detaching factor modification"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567185761369088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Behavior of two <i>Tannerella forsythia</i> strains and their cell surface mutants in multispecies oral biofilms"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861707397389440","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The Association between Tannerella forsythia and the Onset of Fever in Older Nursing Home Residents: A Prospective Cohort Study"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204414112768","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Involvement of <i>Tannerella forsythia</i> virulence factor Forsythia detaching factor in periodontitis"},{"@language":"ja","@value":"<i>Tannerella forsythia</i> 病原因子 Forsythia detaching factor の歯周炎への関与"},{"@value":"Involvement of Tannerella forsythia virulence factor Forsythia detaching factor in periodontitis"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206322901376","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Immunocytochemical approach for surface layer proteins of freeze-substituted Tannerella forsythensis by energy-filtering transmission electron microscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679566078592","@type":"Article","resourceType":"紀要論文(departmental bulletin paper)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Investigation of Subgingival Profile of Periodontopathic Bacteria Using Polymerase Chain 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