{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699994846740992.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1042/bj3350301"}},{"identifier":{"@type":"URI","@value":"https://portlandpress.com/biochemj/article-pdf/335/2/301/632595/bj3350301.pdf"}},{"identifier":{"@type":"PMID","@value":"9761727"}}],"dc:title":[{"@value":"Evidence for the presence of multiple forms of Sph kinase in human platelets"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>The intracellular distribution of sphingosine (Sph) kinase activity was examined in human platelets. A large proportion (72%) of the total activity was found to be associated with the membrane fraction, and the membrane-associated fraction had higher specific activity compared with the cytosolic enzyme. Most of the membrane-associated activity could be extracted with 1 M NaCl. The cytosolic activity was unstable upon heat treatment, with 80% of the activity being lost during incubation at 45 °C for 1 h, whereas the NaCl-extractable fraction was stable under the same conditions. When subjected to Mono Q column chromatography, the cytosolic fraction produced two activity peaks and the NaCl-extractable fraction gave a single peak. These three Sph kinase activities showed different responses to stimulation by β-octylglucoside and inhibition by N,N-dimethylsphingosine and l-threo-dihydrosphingosine, suggesting the presence of multiple enzyme forms in human platelets.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699994846740993","@type":"Researcher","foaf:name":[{"@value":"Yoshiko BANNO"}],"jpcoar:affiliationName":[{"@value":"Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500-8705, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994846740995","@type":"Researcher","foaf:name":[{"@value":"Makiko KATO"}],"jpcoar:affiliationName":[{"@value":"Laboratory of Biochemistry, Gifu Pharmaceutical University, Mitahora-higashi, Gifu 502-0003, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994846740992","@type":"Researcher","foaf:name":[{"@value":"Akira HARA"}],"jpcoar:affiliationName":[{"@value":"Laboratory of Biochemistry, Gifu Pharmaceutical University, Mitahora-higashi, Gifu 502-0003, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699994846740994","@type":"Researcher","foaf:name":[{"@value":"Yoshinori NOZAWA"}],"jpcoar:affiliationName":[{"@value":"Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500-8705, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"02646021"},{"@type":"EISSN","@value":"14708728"}],"prism:publicationName":[{"@value":"Biochemical Journal"}],"dc:publisher":[{"@value":"Portland Press Ltd."}],"prism:publicationDate":"1998-10-15","prism:volume":"335","prism:number":"2","prism:startingPage":"301","prism:endingPage":"304"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"https://portlandpress.com/biochemj/article-pdf/335/2/301/632595/bj3350301.pdf"}],"createdAt":"2015-08-10","modifiedAt":"2021-11-24","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Blood%20Platelets","dc:title":"Blood Platelets"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Extracts","dc:title":"Cell Extracts"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Membrane","dc:title":"Cell Membrane"},{"@id":"https://cir.nii.ac.jp/all?q=Sodium%20Chloride","dc:title":"Sodium Chloride"},{"@id":"https://cir.nii.ac.jp/all?q=Isoenzymes","dc:title":"Isoenzymes"},{"@id":"https://cir.nii.ac.jp/all?q=Phosphotransferases%20(Alcohol%20Group%20Acceptor)","dc:title":"Phosphotransferases (Alcohol Group Acceptor)"},{"@id":"https://cir.nii.ac.jp/all?q=Cytosol","dc:title":"Cytosol"},{"@id":"https://cir.nii.ac.jp/all?q=Glucosides","dc:title":"Glucosides"},{"@id":"https://cir.nii.ac.jp/all?q=Sphingosine","dc:title":"Sphingosine"},{"@id":"https://cir.nii.ac.jp/all?q=Enzyme%20Stability","dc:title":"Enzyme Stability"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Chromatography,%20Liquid","dc:title":"Chromatography, Liquid"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360848656925751040","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Sphingosine kinase 1 expression is downregulated during differentiation of Friend cells due to decreased c-MYB"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680209818880","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"スフィンゴ脂質代謝と癌の分子標的治療薬"},{"@language":"en","@value":"Sphingolipid Metabolism and Molecular Target Drugs for Cancer"},{"@language":"ja-Kana","@value":"スフィンゴ シシツ タイシャ ト ガン ノ ブンシ ヒョウテキ チリョウヤク"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1042/bj3350301"},{"@type":"OPENAIRE","@value":"doi_dedup___::f5ef647568d94f160a368282425269a3"},{"@type":"CROSSREF","@value":"10.1016/j.bbamcr.2013.01.001_references_DOI_TlJJ0YJwT1myYVlZhhDMqexmv5C"},{"@type":"CROSSREF","@value":"10.5939/sjws.17003_references_DOI_TlJJ0YJwT1myYVlZhhDMqexmv5C"}]}