{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699995011666432.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/em.20560"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fem.20560"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/em.20560"}},{"identifier":{"@type":"PMID","@value":"20209648"}}],"dc:title":[{"@value":"Genotoxicity of acrylamide in vitro: Acrylamide is not metabolically activated in standard in vitro systems"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>The recent finding that acrylamide (AA), a genotoxic rodent carcinogen, is formed during the frying or baking of a variety of foods raises human health concerns. AA is known to be metabolized by cytochrome P450 2E1 (CYP2E1) to glycidamide (GA), which is responsible for AA's in vivo genotoxicity and probable carcinogenicity. In in‐vitro mammalian cell tests, however, AA genotoxicity is not enhanced by rat liver S9 or a human liver microsomal fraction. In an attempt to demonstrate the in vitro expression of AA genotoxicity, we employed<jats:italic>Salmonella</jats:italic>strains and human cell lines that overexpress human CYP2E1. In the<jats:italic>umu</jats:italic>test, however, AA was not genotoxic in the CYP2E1‐expressing<jats:italic>Salmonella</jats:italic>strain or its parental strain. Moreover, a transgenic human lymphoblastoid cell line overexpressing CYP2E1 (h2E1v2) and its parental cell line (AHH‐1) both showed equally weak cytotoxic and genotoxic responses to high (>1 mM) AA concentrations. The DNA adduct N7‐GA‐Gua, which is detected in liver following AA treatment in vivo, was not substantially formed in the in vitro system. These results indicate that AA was not metabolically activated to GA in vitro. Thus, AA is not relevantly genotoxic in vitro, although its in vivo genotoxicity was clearly demonstrated. Environ. Mol. Mutagen. 52:11–19, 2011. © 2010 Wiley‐Liss, Inc.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699995011666437","@type":"Researcher","foaf:name":[{"@value":"Naoki Koyama"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995011666433","@type":"Researcher","foaf:name":[{"@value":"Manabu Yasui"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995011666438","@type":"Researcher","foaf:name":[{"@value":"Yoshimitsu Oda"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995011666560","@type":"Researcher","foaf:name":[{"@value":"Satoshi Suzuki"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995011666436","@type":"Researcher","foaf:name":[{"@value":"Tetsuo Satoh"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995011666434","@type":"Researcher","foaf:name":[{"@value":"Takuya Suzuki"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995011666440","@type":"Researcher","foaf:name":[{"@value":"Tomonari Matsuda"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995011666439","@type":"Researcher","foaf:name":[{"@value":"Shuichi Masuda"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995011666432","@type":"Researcher","foaf:name":[{"@value":"Naohide Kinae"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995011666435","@type":"Researcher","foaf:name":[{"@value":"Masamitsu Honma"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"08936692"},{"@type":"EISSN","@value":"10982280"}],"prism:publicationName":[{"@value":"Environmental and Molecular Mutagenesis"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2011-01","prism:volume":"52","prism:number":"1","prism:startingPage":"11","prism:endingPage":"19"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fem.20560"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/em.20560"}],"createdAt":"2010-03-07","modifiedAt":"2025-02-19","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Acrylamide","dc:title":"Acrylamide"},{"@id":"https://cir.nii.ac.jp/all?q=Blotting,%20Western","dc:title":"Blotting, Western"},{"@id":"https://cir.nii.ac.jp/all?q=Cytochrome%20P-450%20CYP2E1","dc:title":"Cytochrome P-450 CYP2E1"},{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Adducts","dc:title":"DNA Adducts"},{"@id":"https://cir.nii.ac.jp/all?q=Salmonella","dc:title":"Salmonella"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line,%20Tumor","dc:title":"Cell Line, Tumor"},{"@id":"https://cir.nii.ac.jp/all?q=Microsomes,%20Liver","dc:title":"Microsomes, Liver"},{"@id":"https://cir.nii.ac.jp/all?q=Epoxy%20Compounds","dc:title":"Epoxy Compounds"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Damage","dc:title":"DNA Damage"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360285707357259264","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Evaluation of mutagenicity of acrylamide using RBC Pig-a and PIGRET assays by single peroral dose in rats"}]},{"@id":"https://cir.nii.ac.jp/crid/1360286989656746368","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Enhancing the sensitivity of the thymidine kinase assay by using <scp>DNA</scp> repair‐deficient human <scp>TK6</scp> cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360298338435149696","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Toxicity of combined exposure to acrylamide and Staphylococcus aureus"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205257110400","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Genotoxicity of Acrylamide and Glycidamide: A Review of the Studies by <i>HPRT</i> Gene and <i>TK</i> Gene Mutation Assays"},{"@value":"Genotoxicity of Acrylamide and Glycidamide : A Review of the Studies by HPRT Gene and TK Gene Mutation Assays"}]},{"@id":"https://cir.nii.ac.jp/crid/2050870367078651136","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effect of sampling time on somatic and germ cell mutations induced by acrylamide in gpt delta mice"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1002/em.20560"},{"@type":"OPENAIRE","@value":"doi_dedup___::0cb81252f113fe8dd9c0ab56893bfc64"},{"@type":"CROSSREF","@value":"10.1002/em.22371_references_DOI_GPiKkkJIWKOU98RQUlwFkZuKQr2"},{"@type":"CROSSREF","@value":"10.3123/jemsge.34.1_references_DOI_GPiKkkJIWKOU98RQUlwFkZuKQr2"},{"@type":"CROSSREF","@value":"10.1016/j.toxrep.2022.04.018_references_DOI_GPiKkkJIWKOU98RQUlwFkZuKQr2"},{"@type":"CROSSREF","@value":"10.1186/s41021-021-00175-5_references_DOI_GPiKkkJIWKOU98RQUlwFkZuKQr2"},{"@type":"CROSSREF","@value":"10.1016/j.mrgentox.2015.12.005_references_DOI_GPiKkkJIWKOU98RQUlwFkZuKQr2"}]}