{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699995086367104.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1021/bc700205t"}},{"identifier":{"@type":"URI","@value":"https://pubs.acs.org/doi/pdf/10.1021/bc700205t"}},{"identifier":{"@type":"PMID","@value":"17718534"}}],"dc:title":[{"@value":"Design of Aminated Poly(1-vinylimidazole) for a New pH-Sensitive Polycation To Enhance Cell-Specific Gene Delivery"}],"description":[{"notation":[{"@value":"Poly(1-vinylimidazole) (PVIm) with aminoethyl groups has been synthesized as a new pH-sensitive polycation to enhance cell-specific gene delivery. The resulting aminated PVIm (PVIm-NH2) was water-soluble despite deprotonation of the imidazole groups at physiological pH, as determined by acid-base titration and solution turbidity measurement. Hemolysis assay showed that PVIm-NH2 enhanced membrane disruptive ability at endosomal pH, owing to the protonation of the imidazole groups with a pKa value around 6.0. Agarose gel retardation assay proved that the introduced aminoethyl groups worked as anchor groups to retain DNA. Furthermore, the ternary complex of DNA, PVIm-NH2, and a poly(L-lysine) conjugated with lactose molecules, PLL-Lac, at pH 7.4 dissociated the PLL-Lac polycation by protonation of the imidazole groups of PVIm-NH2 at pH 6.0. The resulting PVIm-NH2/DNA binary complexes easily released DNA, as compared with the PLL-Lac/PVIm-NH2/DNA ternary complex, which was examined by competitive exchange with dextran sulfate. By using PVIm-NH2 as a pH-sensitive DNA carrier, as well as PLL-Lac as a cell-targeting DNA carrier, the resulting ternary complex specifically mediated the gene expression, which depended on the protonation of the imidazole groups, on human hepatoma HepG2 cells with asialoglycoprotein receptors. These results suggest that the cell-specific gene delivery mediated by PLL-Lac was enhanced by PVIm-NH2 as a new pH-sensitive polycation."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699995086367106","@type":"Researcher","foaf:name":[{"@value":"Shoichiro Asayama"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995086367107","@type":"Researcher","foaf:name":[{"@value":"Takashi Sekine"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995086367105","@type":"Researcher","foaf:name":[{"@value":"Hiroyoshi Kawakami"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995086367104","@type":"Researcher","foaf:name":[{"@value":"Shoji Nagaoka"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"10431802"},{"@type":"EISSN","@value":"15204812"}],"prism:publicationName":[{"@value":"Bioconjugate Chemistry"}],"dc:publisher":[{"@value":"American Chemical Society (ACS)"}],"prism:publicationDate":"2007-08-25","prism:volume":"18","prism:number":"5","prism:startingPage":"1662","prism:endingPage":"1667"},"reviewed":"false","url":[{"@id":"https://pubs.acs.org/doi/pdf/10.1021/bc700205t"}],"createdAt":"2007-08-25","modifiedAt":"2023-03-14","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Lactose","dc:title":"Lactose"},{"@id":"https://cir.nii.ac.jp/all?q=Hemolysis","dc:title":"Hemolysis"},{"@id":"https://cir.nii.ac.jp/all?q=Nephelometry%20and%20Turbidimetry","dc:title":"Nephelometry and Turbidimetry"},{"@id":"https://cir.nii.ac.jp/all?q=Polyamines","dc:title":"Polyamines"},{"@id":"https://cir.nii.ac.jp/all?q=Tumor%20Cells,%20Cultured","dc:title":"Tumor Cells, Cultured"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Polylysine","dc:title":"Polylysine"},{"@id":"https://cir.nii.ac.jp/all?q=Amination","dc:title":"Amination"},{"@id":"https://cir.nii.ac.jp/all?q=Electrophoresis,%20Agar%20Gel","dc:title":"Electrophoresis, Agar Gel"},{"@id":"https://cir.nii.ac.jp/all?q=Drug%20Carriers","dc:title":"Drug Carriers"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Transfer%20Techniques","dc:title":"Gene Transfer Techniques"},{"@id":"https://cir.nii.ac.jp/all?q=Imidazoles","dc:title":"Imidazoles"},{"@id":"https://cir.nii.ac.jp/all?q=Titrimetry","dc:title":"Titrimetry"},{"@id":"https://cir.nii.ac.jp/all?q=Water","dc:title":"Water"},{"@id":"https://cir.nii.ac.jp/all?q=DNA","dc:title":"DNA"},{"@id":"https://cir.nii.ac.jp/all?q=Hydrogen-Ion%20Concentration","dc:title":"Hydrogen-Ion Concentration"},{"@id":"https://cir.nii.ac.jp/all?q=Polyelectrolytes","dc:title":"Polyelectrolytes"},{"@id":"https://cir.nii.ac.jp/all?q=Solubility","dc:title":"Solubility"},{"@id":"https://cir.nii.ac.jp/all?q=Biological%20Assay","dc:title":"Biological Assay"},{"@id":"https://cir.nii.ac.jp/all?q=Polyvinyls","dc:title":"Polyvinyls"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004230247219712","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Tuning of the methylimidazolium/imidazole balance in polycations for gene carrier"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283691858316928","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Intracellular co-delivery of zinc ions and plasmid DNA for enhancing gene transfection 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