{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699995129204864.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/s1568-7864(02)00014-9"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S1568786402000149?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S1568786402000149?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"12530376"}}],"dc:title":[{"@value":"Mutagenic target for hydroxyl radicals generated in Escherichia coli mutant deficient in Mn- and Fe-superoxide dismutases and Fur, a repressor for iron-uptake systems"}],"description":[{"notation":[{"@value":"We previously reported that mutations in Mn- and Fe-superoxide dismutases and Fur, a repressor for iron uptake systems, simulated generation of hydroxyl radicals, and caused hypermutability in Escherichia coli. The predominant type of spontaneous mutation was GC --TA, followed by AT --CG, suggesting the involvement of 7,8-dihydro-8-oxoguanine (8-oxoG) and 1,2-dihydro-2-oxoadenine (2-oxoA) in DNA as well as 7,8-dihydro-8-oxodeoxyguanosine triphosphate (8-oxodGTP) and 1,2-dihydro-2-oxodeoxyadenosine triphosphate (2-oxodATP) in the nucleotide pool. To determine the targets contributing to oxidative mutagenesis, DNA or nucleotides, we characterized spontaneous mutations and compared the distribution to those in mutMY and mutT strains, in which GC --TA and AT --CG were predominantly induced, respectively. The hotspots and sequence contexts where AT --CG occurred frequently in sodAB fur strain were almost identical to those in mutT strain,whereas, those where GC --TA occurred frequently in sodAB fur strain were quite different from those in mutMY strain. These observations suggested that AT --CG is due to 8-oxodGTP, while GC --TA is produced by some other lesion(s). The 2-oxodATP is also a major oxidative lesion in nucleotides, and strongly induces GC --TA. The expression of cDNA for MTH1, which can hydrolyze 2-oxodATP as well as 8-oxodGTP, partially but significantly, suppressed the GC --TA mutator phenotype of the sodAB fur strain, whereas, it did not for the mutMY strain. Additionally, the sequence contextby 2-oxodATP in E. coli was similar to that in sodAB fur strain. These results suggested that the targets contributing to oxidative mutagenesis in sodAB fur strain are nucleotides such as dGTP and dATP, rather than DNA."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380298344737647746","@type":"Researcher","foaf:name":[{"@value":"Tatsuo Nunoshiba"}]},{"@id":"https://cir.nii.ac.jp/crid/1380298344737647745","@type":"Researcher","foaf:name":[{"@value":"Takashi Watanabe"}]},{"@id":"https://cir.nii.ac.jp/crid/1380298344737647744","@type":"Researcher","foaf:name":[{"@value":"Yusaku Nakabeppu"}]},{"@id":"https://cir.nii.ac.jp/crid/1380298344737647747","@type":"Researcher","foaf:name":[{"@value":"Kazuo Yamamoto"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"15687864"},{"@type":"PISSN","@value":"https://id.crossref.org/issn/15687864"}],"prism:publicationName":[{"@value":"DNA Repair"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2002-05","prism:volume":"1","prism:number":"5","prism:startingPage":"411","prism:endingPage":"418"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","https://www.elsevier.com/legal/tdmrep-license"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S1568786402000149?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S1568786402000149?httpAccept=text/plain"}],"createdAt":"2002-10-14","modifiedAt":"2025-10-27","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Repair","dc:title":"DNA Repair"},{"@id":"https://cir.nii.ac.jp/all?q=Iron","dc:title":"Iron"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Sequence%20Data","dc:title":"Molecular Sequence Data"},{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Glycosylases","dc:title":"DNA Glycosylases"},{"@id":"https://cir.nii.ac.jp/all?q=Deoxyadenine%20Nucleotides","dc:title":"Deoxyadenine Nucleotides"},{"@id":"https://cir.nii.ac.jp/all?q=Bacterial%20Proteins","dc:title":"Bacterial Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=RNA,%20Transfer","dc:title":"RNA, Transfer"},{"@id":"https://cir.nii.ac.jp/all?q=Escherichia%20coli","dc:title":"Escherichia coli"},{"@id":"https://cir.nii.ac.jp/all?q=Pyrophosphatases","dc:title":"Pyrophosphatases"},{"@id":"https://cir.nii.ac.jp/all?q=Genes,%20Suppressor","dc:title":"Genes, Suppressor"},{"@id":"https://cir.nii.ac.jp/all?q=N-Glycosyl%20Hydrolases","dc:title":"N-Glycosyl Hydrolases"},{"@id":"https://cir.nii.ac.jp/all?q=Manganese","dc:title":"Manganese"},{"@id":"https://cir.nii.ac.jp/all?q=Base%20Sequence","dc:title":"Base Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Hydroxyl%20Radical","dc:title":"Hydroxyl Radical"},{"@id":"https://cir.nii.ac.jp/all?q=Superoxide%20Dismutase","dc:title":"Superoxide Dismutase"},{"@id":"https://cir.nii.ac.jp/all?q=Escherichia%20coli%20Proteins","dc:title":"Escherichia coli Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Deoxyguanine%20Nucleotides","dc:title":"Deoxyguanine Nucleotides"},{"@id":"https://cir.nii.ac.jp/all?q=AT%20Rich%20Sequence","dc:title":"AT Rich Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Phosphoric%20Monoester%20Hydrolases","dc:title":"Phosphoric Monoester Hydrolases"},{"@id":"https://cir.nii.ac.jp/all?q=GC%20Rich%20Sequence","dc:title":"GC Rich Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Repressor%20Proteins","dc:title":"Repressor Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=DNA-Formamidopyrimidine%20Glycosylase","dc:title":"DNA-Formamidopyrimidine Glycosylase"},{"@id":"https://cir.nii.ac.jp/all?q=Mutagenesis","dc:title":"Mutagenesis"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360567185753542144","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"<i><scp>E</scp>scherichia coli</i><scp>DNA</scp> polymerase <scp>III</scp> is responsible for 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