{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699995452926976.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1038/srep08572"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/srep08572"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/srep08572.pdf"}}],"dc:title":[{"@value":"Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>Chinese hamster ovary (CHO) cells are the most widely used mammalian hosts for production of therapeutic proteins. However, development of recombinant CHO cell lines has been hampered by unstable and variable transgene expression caused by random integration. Here we demonstrate efficient targeted gene integration into site-specific loci in CHO cells using CRISPR/Cas9 genome editing system and compatible donor plasmid harboring a gene of interest (GOI) and short homology arms. This strategy has enabled precise insertion of a 3.7-kb gene expression cassette at defined loci in CHO cells following a simple drug-selection, resulting in homogeneous transgene expression. Taken together, the results displayed here can help pave the way for the targeting of GOI to specific loci in CHO cells, increasing the likelihood of generating isogenic cell lines with consistent protein production.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699995452926979","@type":"Researcher","foaf:name":[{"@value":"Jae Seong Lee"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995452926978","@type":"Researcher","foaf:name":[{"@value":"Thomas Beuchert Kallehauge"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995452926976","@type":"Researcher","foaf:name":[{"@value":"Lasse Ebdrup Pedersen"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995452926977","@type":"Researcher","foaf:name":[{"@value":"Helene Faustrup Kildegaard"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"20452322"}],"prism:publicationName":[{"@value":"Scientific Reports"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2015-02-25","prism:volume":"5","prism:number":"1","prism:startingPage":"8572"},"reviewed":"false","dc:rights":["https://creativecommons.org/licenses/by/4.0","https://creativecommons.org/licenses/by/4.0"],"url":[{"@id":"https://www.nature.com/articles/srep08572"},{"@id":"https://www.nature.com/articles/srep08572.pdf"}],"createdAt":"2015-02-25","modifiedAt":"2023-01-06","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002218449507456","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848658388171904","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Combinations of chromosome transfer and genome editing for the development of cell/animal models of human disease and humanized animal models"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848658410560512","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"CRISPR/Cas9-induced transgene insertion and telomere-associated truncation of a single human chromosome for chromosome engineering in CHO and A9 cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848664422907776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids"}]},{"@id":"https://cir.nii.ac.jp/crid/1360857593669770752","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"CRISPR-derived genome editing technologies for metabolic engineering"}]},{"@id":"https://cir.nii.ac.jp/crid/1521699230771650176","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1038/srep08572"},{"@type":"CROSSREF","@value":"10.1126/science.aaf8729_references_DOI_7rZQT1ZejjFiDqUjAnmNImQfSSe"},{"@type":"CROSSREF","@value":"10.1016/j.jbiosc.2017.12.003_references_DOI_7rZQT1ZejjFiDqUjAnmNImQfSSe"},{"@type":"CROSSREF","@value":"10.1038/s10038-017-0378-7_references_DOI_7rZQT1ZejjFiDqUjAnmNImQfSSe"},{"@type":"CROSSREF","@value":"10.1038/s41598-017-10418-7_references_DOI_7rZQT1ZejjFiDqUjAnmNImQfSSe"},{"@type":"CROSSREF","@value":"10.3390/ijms161023849_references_DOI_7rZQT1ZejjFiDqUjAnmNImQfSSe"},{"@type":"CROSSREF","@value":"10.1016/j.ymben.2020.12.002_references_DOI_7rZQT1ZejjFiDqUjAnmNImQfSSe"}]}