{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699995471665792.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1113/jphysiol.1991.sp018802"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1113%2Fjphysiol.1991.sp018802"}},{"identifier":{"@type":"URI","@value":"https://physoc.onlinelibrary.wiley.com/doi/pdf/10.1113/jphysiol.1991.sp018802"}}],"dc:title":[{"@value":"Effect of external Cd2+ and other divalent cations on carbachol‐activated non‐selective cation channels in guinea‐pig ileum."}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>1. Inhibitory actions of the external divalent cations, Cd2+, Ni2+, Mn2+ and Co2+ on carbachol (CCh)‐induced inward current (Ins,ACh) were investigated in caesium aspartate‐loaded single longitudinal smooth muscle cells of guinea‐pig ileum, using a rapid solution‐switching device, under voltage‐clamped conditions. 2. Cd2+ (20‐1000 microM), when added to the external solution, reduced Ins,ACh rapidly and reversibly. This effect occurred dose dependently, the relationship being adequately described by a Michaelis‐Menten equation with a Hill coefficient (n) of 1.0 and a dissociation constant (Kd) of 98 microM. 3. The inhibitory action of Cd2+ was associated neither with agonist concentration (CCh) nor with changes in reversal potential, and was voltage independent. 4. The appearance and removal of the effect of Cd2+ were both rapid (a few hundred milliseconds), in sharp contrast with a relatively slow time course of atropine or CCh action (of the order of seconds). 5. Other divalent cations (Ni2+, Mn2+, Co2+, Mg2+) applied externally also suppressed Ins,ACh, but less potently. The sequence of apparent potency was Cd2+ (98 microM) greater than or equal to Ni2+ (131 microM) much greater than Co2+ (700 microM) greater than or equal to Mn2+ (1000 microM) much greater than Mg2+ (approximately 10 mM). 6. External Ca2+ increased Ins,ACh dose dependently and antagonized the inhibitory effect of Cd2+. However, this effect may not be a simple competition with Cd2+. 7. These results show that external divalent cations strongly modulate Ins,ACh channels, possibly through direct interaction with the channel protein.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380006431887722116","@type":"Researcher","foaf:name":[{"@value":"R Inoue"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00223751"},{"@type":"EISSN","@value":"14697793"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/00223751"}],"prism:publicationName":[{"@value":"The Journal of Physiology"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"1991-10","prism:volume":"442","prism:number":"1","prism:startingPage":"447","prism:endingPage":"463"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1113%2Fjphysiol.1991.sp018802"},{"@id":"https://physoc.onlinelibrary.wiley.com/doi/pdf/10.1113/jphysiol.1991.sp018802"}],"createdAt":"2014-12-17","modifiedAt":"2023-10-23","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1390001205057026560","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Nonselective Cation Channels Activated by the Stimulation of Muscarinic Receptors in Mammalian Gastric Smooth Muscle."}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205057879552","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effects of inhibitors of nonselective cation channels on the acetylcholine-induced depolarization of circular smooth muscle from the guinea-pig stomach antrum"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679261306112","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The Properties of Carbachol-Activated Nonselective Cation Channels at the Single Channel Level in Guinea Pig Gastric Myocytes."}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679262391552","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Characterization of a Palytoxin-Induced Non-selective Cation Channel in Mouse Megakaryocytes"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1113/jphysiol.1991.sp018802"},{"@type":"CROSSREF","@value":"10.1540/jsmr.39.231_references_DOI_11PtOuPxD1jXI3wO9XiucHXI7La"},{"@type":"CROSSREF","@value":"10.1540/jsmr.41.313_references_DOI_11PtOuPxD1jXI3wO9XiucHXI7La"},{"@type":"CROSSREF","@value":"10.1254/jjp.81.200_references_DOI_11PtOuPxD1jXI3wO9XiucHXI7La"},{"@type":"CROSSREF","@value":"10.1254/jjp.85.291_references_DOI_11PtOuPxD1jXI3wO9XiucHXI7La"}]}