{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699995572393344.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1091/mbc.e11-03-0271"}}],"dc:title":[{"@value":"The CSC is required for complete radial spoke assembly and wild-type ciliary motility"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>The ubiquitous calcium binding protein, calmodulin (CaM), plays a major role in regulating the motility of all eukaryotic cilia and flagella. We previously identified a CaM and Spoke associated Complex (CSC) and provided evidence that this complex mediates regulatory signals between the radial spokes and dynein arms. We have now used an artificial microRNA (amiRNA) approach to reduce expression of two CSC subunits in Chlamydomonas. For all amiRNA mutants, the entire CSC is lacking or severely reduced in flagella. Structural studies of mutant axonemes revealed that assembly of radial spoke 2 is defective. Furthermore, analysis of both flagellar beating and microtubule sliding in vitro demonstrates that the CSC plays a critical role in modulating dynein activity. Our results not only indicate that the CSC is required for spoke assembly and wild-type motility, but also provide evidence for heterogeneity among the radial spokes.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699995572393348","@type":"Researcher","foaf:name":[{"@value":"Erin E. Dymek"}],"jpcoar:affiliationName":[{"@value":"Department of Biological Sciences, Dartmouth College, Hanover, NH 03755"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995572393345","@type":"Researcher","foaf:name":[{"@value":"Thomas Heuser"}],"jpcoar:affiliationName":[{"@value":"Department of Biology, Brandeis University, Waltham, MA 02453"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995572393347","@type":"Researcher","foaf:name":[{"@value":"Daniela Nicastro"}],"jpcoar:affiliationName":[{"@value":"Department of Biology, Brandeis University, Waltham, MA 02453"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995572393346","@type":"Researcher","foaf:name":[{"@value":"Elizabeth F. Smith"}],"jpcoar:affiliationName":[{"@value":"Department of Biological Sciences, Dartmouth College, Hanover, NH 03755"}]}],"contributor":[{"@id":"https://cir.nii.ac.jp/crid/1381699995572393344","@type":"Researcher","foaf:name":[{"@value":"Erika L. F. Holzbaur"}],"role":"editor"}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"10591524"},{"@type":"EISSN","@value":"19394586"}],"prism:publicationName":[{"@value":"Molecular Biology of the Cell"}],"dc:publisher":[{"@value":"American Society for Cell Biology (ASCB)"}],"prism:publicationDate":"2011-07-15","prism:volume":"22","prism:number":"14","prism:startingPage":"2520","prism:endingPage":"2531"},"reviewed":"false","createdAt":"2011-05-25","modifiedAt":"2021-11-23","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360013168846059520","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Composition and function of ciliary inner‐dynein‐arm subunits studied in <i>Chlamydomonas reinhardtii</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021389796688512","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Functional and structural significance of the inner‐arm‐dynein subspecies d in ciliary motility"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283692363489920","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The MIA complex is a conserved and novel dynein regulator essential for normal ciliary motility"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283692363552384","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Mechanosignaling between central apparatus and radial spokes controls axonemal dynein activity"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1091/mbc.e11-03-0271"},{"@type":"CROSSREF","@value":"10.1002/cm.21662_references_DOI_9DuHY38s9eHync5IilcJEkyW8z"},{"@type":"CROSSREF","@value":"10.1002/cm.21828_references_DOI_9DuHY38s9eHync5IilcJEkyW8z"},{"@type":"CROSSREF","@value":"10.1083/jcb.201211048_references_DOI_Kiv2MMmB9lIQZynLKTafktwHzLP"},{"@type":"CROSSREF","@value":"10.1083/jcb.201312014_references_DOI_Kiv2MMmB9lIQZynLKTafktwHzLP"}]}