{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699995633929984.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/lsm.1900090203"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flsm.1900090203"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/lsm.1900090203"}},{"identifier":{"@type":"NAID","@value":"30025968619"}}],"dc:title":[{"@value":"In vitro photosensitization I. Cellular uptake and subcellular localization of mono‐L‐aspartyl chlorin e<sub>6</sub>, chloro‐aluminum sulfonated phthalocyanine, and photofrin II"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>The mechanisms of cellular uptake, subcellular localization, and cellular retention kinetics of the photosensitizers photofrin II (PfII), mono‐<jats:sc>L</jats:sc>‐aspartyl chlorin e<jats:sub>6</jats:sub> (MACE), and chloro‐aluminum sulfonated phthalocyanine (CASPc) are reported in this paper. Each photosensitizer's cellular uptake mechanism was determined by preferentially inhibiting endocytosis by chilling cells to 2°C, while allowing diffusion across the membrane. Subcellular localization was studied by computer‐enhanced low‐light level video fluorescence microscopy, while flow cytometry was used to determine uptake and retention kinetics. The results indicate that PfII enters the cell primarily by diffusion across the membrane, whereas MACE and CASPc enter the cell through endocytosis.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699995633929985","@type":"Researcher","foaf:name":[{"@value":"W. Gregory Roberts"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995633929984","@type":"Researcher","foaf:name":[{"@value":"Michael W. Berns"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"01968092"},{"@type":"EISSN","@value":"10969101"},{"@type":"PISSN","@value":"https://id.crossref.org/issn/01968092"}],"prism:publicationName":[{"@value":"Lasers in Surgery and Medicine"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"1989-01","prism:volume":"9","prism:number":"2","prism:startingPage":"90","prism:endingPage":"101"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flsm.1900090203"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/lsm.1900090203"}],"createdAt":"2007-03-03","modifiedAt":"2023-10-22","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360285705172291968","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Tetrakis(p-Carboranylthio-Tetrafluorophenyl)Chlorin (TPFC): 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