{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699995658232960.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/tpj.12046"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Ftpj.12046"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/tpj.12046"}}],"dc:title":[{"@value":"Impaired sterol ester synthesis alters the response of <i>Arabidopsis thaliana</i> to <i>Phytophthora infestans</i>"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Summary</jats:title><jats:p>Non‐host resistance of <jats:italic>Arabidopsis thaliana</jats:italic> against <jats:italic>Phytophthora infestans</jats:italic>, the causal agent of late blight disease of potato, depends on efficient extracellular pre‐ and post‐invasive resistance responses. Pre‐invasive resistance against <jats:italic>P. infestans</jats:italic> requires the myrosinase <jats:styled-content style=\"fixed-case\">PEN</jats:styled-content>2. To identify additional genes involved in non‐host resistance to <jats:italic>P. infestans</jats:italic>, a genetic screen was performed by re‐mutagenesis of <jats:italic>pen2</jats:italic> plants. Fourteen independent mutants were isolated that displayed an <jats:italic>enhanced response to Phytophthora</jats:italic> (<jats:italic>erp</jats:italic>) phenotype. Upon inoculation with <jats:italic>P. infestans</jats:italic>, two mutants, <jats:italic>pen2‐1 erp1‐3</jats:italic> and <jats:italic>pen2‐1 erp1‐4</jats:italic>, showed an enhanced rate of mesophyll cell death and produced excessive callose deposits in the mesophyll cell layer. <jats:italic><jats:styled-content style=\"fixed-case\">ERP</jats:styled-content>1</jats:italic> encodes a phospholipid:sterol acyltransferase (<jats:styled-content style=\"fixed-case\">PSAT</jats:styled-content>1) that catalyzes the formation of sterol esters. Consistent with this, the tested T‐<jats:styled-content style=\"fixed-case\">DNA</jats:styled-content> insertion lines of <jats:italic><jats:styled-content style=\"fixed-case\">PSAT</jats:styled-content>1</jats:italic> are phenocopies of <jats:italic>erp1</jats:italic> plants. Sterol ester levels are highly reduced in all <jats:italic>erp1/psat1</jats:italic> mutants, whereas sterol glycoside levels are increased twofold. Excessive callose deposition occurred independently of <jats:styled-content style=\"fixed-case\">PMR</jats:styled-content>4/<jats:styled-content style=\"fixed-case\">GSL</jats:styled-content>5 activity, a known pathogen‐inducible callose synthase. A similar formation of aberrant callose deposits was triggered by the inoculation of <jats:italic>erp1</jats:italic> <jats:italic>psat1</jats:italic> plants with powdery mildew. These results suggest a role for sterol conjugates in cell non‐autonomous defense responses against invasive filamentous pathogens.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699995658233092","@type":"Researcher","foaf:name":[{"@value":"Michaela Kopischke"}],"jpcoar:affiliationName":[{"@value":"Department of Stress and Developmental Biology Leibniz Institute of Plant Biochemistry Weinberg 3 D‐06120 Halle (Saale) Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995658233089","@type":"Researcher","foaf:name":[{"@value":"Lore Westphal"}],"jpcoar:affiliationName":[{"@value":"Department of Stress and Developmental Biology Leibniz Institute of Plant Biochemistry Weinberg 3 D‐06120 Halle (Saale) Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995658232960","@type":"Researcher","foaf:name":[{"@value":"Korbinian Schneeberger"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Biology Max‐Planck‐Institute for Developmental Biology Spemannstrasse 35 D‐72076 Tübingen Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995658232966","@type":"Researcher","foaf:name":[{"@value":"Richard Clark"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Biology Max‐Planck‐Institute for Developmental Biology Spemannstrasse 35 D‐72076 Tübingen Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995658232961","@type":"Researcher","foaf:name":[{"@value":"Stephan Ossowski"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Biology Max‐Planck‐Institute for Developmental Biology Spemannstrasse 35 D‐72076 Tübingen Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995658232963","@type":"Researcher","foaf:name":[{"@value":"Vera Wewer"}],"jpcoar:affiliationName":[{"@value":"Institute of Molecular Physiology and Biotechnology of Plants University of Bonn Karlrobert‐Kreiten‐Strasse 13 53115 Bonn Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995658232964","@type":"Researcher","foaf:name":[{"@value":"René Fuchs"}],"jpcoar:affiliationName":[{"@value":"Department of Plant Cell Biology Georg‐August‐University Göttingen Schwann‐Schleiden Centre Julia‐Lermontowa‐Weg 3 37077 Göttingen Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995658233090","@type":"Researcher","foaf:name":[{"@value":"Jörn Landtag"}],"jpcoar:affiliationName":[{"@value":"Department of Stress and Developmental Biology Leibniz Institute of Plant Biochemistry Weinberg 3 D‐06120 Halle (Saale) Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995658233094","@type":"Researcher","foaf:name":[{"@value":"Gerd Hause"}],"jpcoar:affiliationName":[{"@value":"Martin Luther University Halle‐Wittenberg Biocenter Weinbergweg 22 D‐06120 Halle (Saale) Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995658233091","@type":"Researcher","foaf:name":[{"@value":"Peter Dörmann"}],"jpcoar:affiliationName":[{"@value":"Institute of Molecular Physiology and Biotechnology of Plants University of Bonn Karlrobert‐Kreiten‐Strasse 13 53115 Bonn Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995658233088","@type":"Researcher","foaf:name":[{"@value":"Volker Lipka"}],"jpcoar:affiliationName":[{"@value":"Department of Plant Cell Biology Georg‐August‐University Göttingen Schwann‐Schleiden Centre Julia‐Lermontowa‐Weg 3 37077 Göttingen Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995658233095","@type":"Researcher","foaf:name":[{"@value":"Detlef Weigel"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Biology Max‐Planck‐Institute for Developmental Biology Spemannstrasse 35 D‐72076 Tübingen Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1380016868541405568","@type":"Researcher","foaf:name":[{"@value":"Paul Schulze‐Lefert"}],"jpcoar:affiliationName":[{"@value":"Max Planck Institute for Plant Breeding Research Carl‐von‐Linné‐Weg 10 D‐50829 Köln Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995658233093","@type":"Researcher","foaf:name":[{"@value":"Dierk Scheel"}],"jpcoar:affiliationName":[{"@value":"Department of Stress and Developmental Biology Leibniz Institute of Plant Biochemistry Weinberg 3 D‐06120 Halle (Saale) Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995658232965","@type":"Researcher","foaf:name":[{"@value":"Sabine Rosahl"}],"jpcoar:affiliationName":[{"@value":"Department of Stress and Developmental Biology Leibniz Institute of Plant Biochemistry Weinberg 3 D‐06120 Halle (Saale) Germany"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09607412"},{"@type":"EISSN","@value":"1365313X"}],"prism:publicationName":[{"@value":"The Plant Journal"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2012-12-12","prism:volume":"73","prism:number":"3","prism:startingPage":"456","prism:endingPage":"468"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Ftpj.12046"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/tpj.12046"}],"createdAt":"2012-10-16","modifiedAt":"2023-10-28","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050282810793045888","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Leaf oil bodies are subcellular factories producing antifungal oxylipins."}]},{"@id":"https://cir.nii.ac.jp/crid/1050564289020502016","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"HIGH STEROL ESTER 1 is a key 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