{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699995701547136.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/chem.200600522"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fchem.200600522"}},{"identifier":{"@type":"URI","@value":"https://chemistry-europe.onlinelibrary.wiley.com/doi/pdf/10.1002/chem.200600522"}},{"identifier":{"@type":"PMID","@value":"17136791"}}],"dc:title":[{"@value":"Design of a Practical Fluorescent Probe for Superoxide Based on Protection–Deprotection Chemistry of Fluoresceins with Benzenesulfonyl Protecting Groups"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>A strategy for designing probes based on protection–deprotection chemistry involving fluoresceins and their benzenesulfonyl (BES) derivatives has led to the development of a much more practical superoxide (O<jats:sub>2</jats:sub><jats:sup>−.</jats:sup>) probe than the previously reported bis(2,4‐dinitro‐BES) tetrafluorofluorescein (<jats:bold>6 a</jats:bold>). Examination of various BES derivatives, developed from the starting point of the prototype probe <jats:bold>6 a</jats:bold>, yielded 4,5‐dimethoxy‐2‐nitro‐BES tetrafluorofluorescein (BESSo; <jats:bold>7 j</jats:bold>) as the optimal reagent. A microtiter plate assay with BESSo showed a tenfold improved detection limit for O<jats:sub>2</jats:sub><jats:sup>−.</jats:sup> compared with such an assay based on <jats:bold>6 a</jats:bold>. BESSo showed markedly better specificity for O<jats:sub>2</jats:sub><jats:sup>−.</jats:sup> than for GSH or other reactive oxygen species, and this specificity was significantly higher than that of Fe<jats:sup>2+</jats:sup> and some reducing enzymes. These features have resulted in the development of an assay based on BESSo that is capable of providing more unambiguous results for O<jats:sub>2</jats:sub><jats:sup>−.</jats:sup> release from neutrophils, with or without stimulation by phorbol myristate acetate, as compared with an assay based on <jats:bold>6 a</jats:bold>. Intracellular generation of O<jats:sub>2</jats:sub><jats:sup>−.</jats:sup> in human Jurkat T cells stimulated by butyric acid has been measured by using flow cytometry and fluorescence microscopy utilizing the acetoxymethyl derivative of BESSo.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699995701547140","@type":"Researcher","foaf:name":[{"@value":"Hatsuo Maeda"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995701547137","@type":"Researcher","foaf:name":[{"@value":"Kayoko Yamamoto"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995701547141","@type":"Researcher","foaf:name":[{"@value":"Iho Kohno"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995701547008","@type":"Researcher","foaf:name":[{"@value":"Leila Hafsi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995701547142","@type":"Researcher","foaf:name":[{"@value":"Norio Itoh"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995701547009","@type":"Researcher","foaf:name":[{"@value":"Shinsaku Nakagawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995701547138","@type":"Researcher","foaf:name":[{"@value":"Naoko Kanagawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995701547136","@type":"Researcher","foaf:name":[{"@value":"Keiichiro Suzuki"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995701547139","@type":"Researcher","foaf:name":[{"@value":"Tadayuki Uno"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09476539"},{"@type":"EISSN","@value":"15213765"}],"prism:publicationName":[{"@value":"Chemistry – A European Journal"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2007-02-08","prism:volume":"13","prism:number":"7","prism:startingPage":"1946","prism:endingPage":"1954"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fchem.200600522"},{"@id":"https://chemistry-europe.onlinelibrary.wiley.com/doi/pdf/10.1002/chem.200600522"}],"createdAt":"2006-11-30","modifiedAt":"2025-10-12","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Neutrophils","dc:title":"Neutrophils"},{"@id":"https://cir.nii.ac.jp/all?q=T-Lymphocytes","dc:title":"T-Lymphocytes"},{"@id":"https://cir.nii.ac.jp/all?q=Chemistry,%20Organic","dc:title":"Chemistry, Organic"},{"@id":"https://cir.nii.ac.jp/all?q=Fluoresceins","dc:title":"Fluoresceins"},{"@id":"https://cir.nii.ac.jp/all?q=Kinetics","dc:title":"Kinetics"},{"@id":"https://cir.nii.ac.jp/all?q=Superoxides","dc:title":"Superoxides"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Tetradecanoylphorbol%20Acetate","dc:title":"Tetradecanoylphorbol Acetate"},{"@id":"https://cir.nii.ac.jp/all?q=Sulfonic%20Acids","dc:title":"Sulfonic Acids"},{"@id":"https://cir.nii.ac.jp/all?q=Cells,%20Cultured","dc:title":"Cells, Cultured"},{"@id":"https://cir.nii.ac.jp/all?q=Fluorescent%20Dyes","dc:title":"Fluorescent Dyes"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050282810762960000","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Protective roles of ascorbic acid in oxidative stress induced by depletion of superoxide dismutase in vertebrate 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