{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699995721049600.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/s0142-9612(03)00347-8"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0142961203003478?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0142961203003478?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"12922159"}},{"identifier":{"@type":"NAID","@value":"30003052738"}}],"dc:title":[{"@value":"Polyion complex micelles from plasmid DNA and poly(ethylene glycol)–poly(l-lysine) block copolymer as serum-tolerable polyplex system: physicochemical properties of micelles relevant to gene transfection efficiency"}],"description":[{"notation":[{"@value":"Polyion complex (PIC) micelles composed of the poly(ethylene glycol)-poly(L-lysine) (PEG-PLL) block copolymer and plasmid DNA (pDNA) were investigated in this study from a physicochemical viewpoint to get insight into the structural feature of the PIC micellar vector system to show practical gene transfection efficacy particularly under serum-containing medium. The residual ratio (r) of the lysine units in PEG-PLL to the phosphate units of pDNA in the system significantly affects the size of the PIC micelles evaluated from dynamic light scattering, being decreased from approximately 120 to 80 nm with an increase in the r value for the region with ror = 1.0. The zeta potential of the complexes slightly increased with r in the same region, yet maintained a very small absolute value and leveled off to a few mV at r approximately 2.0. These results suggest that the micelles are most likely to take the core-shell structure with dense PEG palisades surrounding the PIC core to compartmentalize the condensed pDNA. Furthermore, an increasing r value in the region of ror = 1 induces a rearrangement of the stoichiometric complex formed at r=1.0 to the non-stoichiometric complex composed of the excess block copolymer. The association number of pDNA and the block copolymer in the micelle was estimated from the apparent micellar molecular weight determined by static light scattering measurements, indicating that a single pDNA molecule was incorporated in each of the micelles prepared from the PEG (Mw=12,000 g/mol)-PLL (polymerization degree of PLL segment: 48) (12-48) block copolymer at r=2.0. These 12-48/pDNA micelles showed a gene expression comparable to the lipofection toward cultured 293 cells, though 100 microM chloroquine was required in the transfection medium. Notably, even in the presence of serum, the PIC micelles achieved appreciable cellular association to attain a high gene expression, which is in sharp contrast with the drastic decrease in the gene expression for lipoplex system in the presence of serum. A virus-comparable size (approximately 100 nm) with a serum-tolerable property of the PIC micelles indeed suggests their promising feasibility as non-viral gene-vector systems used for clinical gene therapy."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699995721049604","@type":"Researcher","foaf:name":[{"@value":"Keiji Itaka"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995721049601","@type":"Researcher","foaf:name":[{"@value":"Kyosuke Yamauchi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995721049605","@type":"Researcher","foaf:name":[{"@value":"Atsushi Harada"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995721049603","@type":"Researcher","foaf:name":[{"@value":"Kozo Nakamura"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995721049602","@type":"Researcher","foaf:name":[{"@value":"Hiroshi Kawaguchi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995721049600","@type":"Researcher","foaf:name":[{"@value":"Kazunori Kataoka"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"01429612"}],"prism:publicationName":[{"@value":"Biomaterials"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2003-11","prism:volume":"24","prism:number":"24","prism:startingPage":"4495","prism:endingPage":"4506"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","https://www.elsevier.com/legal/tdmrep-license"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0142961203003478?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0142961203003478?httpAccept=text/plain"}],"createdAt":"2003-06-30","modifiedAt":"2025-09-26","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Light","dc:title":"Light"},{"@id":"https://cir.nii.ac.jp/all?q=Biocompatible%20Materials","dc:title":"Biocompatible Materials"},{"@id":"https://cir.nii.ac.jp/all?q=Biological%20Transport","dc:title":"Biological Transport"},{"@id":"https://cir.nii.ac.jp/all?q=DNA","dc:title":"DNA"},{"@id":"https://cir.nii.ac.jp/all?q=Transfection","dc:title":"Transfection"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line","dc:title":"Cell Line"},{"@id":"https://cir.nii.ac.jp/all?q=Polyethylene%20Glycols","dc:title":"Polyethylene Glycols"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Weight","dc:title":"Molecular Weight"},{"@id":"https://cir.nii.ac.jp/all?q=Kinetics","dc:title":"Kinetics"},{"@id":"https://cir.nii.ac.jp/all?q=Spectrometry,%20Fluorescence","dc:title":"Spectrometry, Fluorescence"},{"@id":"https://cir.nii.ac.jp/all?q=Ethidium","dc:title":"Ethidium"},{"@id":"https://cir.nii.ac.jp/all?q=Fluorescence%20Resonance%20Energy%20Transfer","dc:title":"Fluorescence Resonance Energy Transfer"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Scattering,%20Radiation","dc:title":"Scattering, Radiation"},{"@id":"https://cir.nii.ac.jp/all?q=Polylysine","dc:title":"Polylysine"},{"@id":"https://cir.nii.ac.jp/all?q=Micelles","dc:title":"Micelles"},{"@id":"https://cir.nii.ac.jp/all?q=Plasmids","dc:title":"Plasmids"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050001335723707136","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Involvement of activated transcriptional process in efficient gene transfection using unmodified and mannose-modified bubble lipoplexes with ultrasound exposure."},{"@value":"Involvement of activated transcriptional process in the efficient gene transfection using unmodified and mannose-modified bubble lipoplexes with ultrasound exposure"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002214425592448","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Block Copolymer Micellization as a Protection Strategy for DNA Origami"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002215826942208","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Actively-targeted polyion complex micelles stabilized by cholesterol and disulfide cross-linking for systemic delivery of siRNA to solid tumors"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002216000625536","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"In situ quantitative monitoring of polyplexes and polyplex micelles in the blood circulation using intravital real-time confocal laser scanning microscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004232006642688","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Enhanced gene expression promoted by the quantized folding of pDNA within polyplex micelles"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004232204987648","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Evaluation of the influence of ionization states and spacers in the thermotropic phase behaviour of amino acid-based cationic lipids and the transfection efficiency of their assemblies"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004232249052928","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Quick nuclear transportation of siRNA and in vivo hepatic ApoB gene silencing with galactose-bearing polymeric carrier"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004233404261632","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Impact of polyplex micelles installed with cyclic RGD peptide as ligand on gene delivery to vascular lesions"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004233592044800","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Gene expression of ternary complexes through the compaction of nanofiber-polyplexes by mixing with lipofectamine"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004234218339968","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Precise tuning of disulphide crosslinking in mRNA polyplex micelles for optimising extracellular and intracellular nuclease tolerability"}]},{"@id":"https://cir.nii.ac.jp/crid/1360009142754483840","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Synthesis, aggregation and adsorption behaviour of a thermoresponsive pentablock copolymer"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285707235479808","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Antiangiogenic gene therapy of experimental pancreatic tumor by sFlt-1 plasmid DNA carried by RGD-modified crosslinked polyplex micelles"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285707235570560","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effective transgene expression without toxicity by intraperitoneal administration of PEG-detachable polyplex micelles in mice with peritoneal dissemination"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285707450193920","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Polymeric micelles for nano-scale drug delivery"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285708141877760","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Alkylimidazolium End-Modified Poly(ethylene glycol) To Form the Mono-ion Complex with Plasmid DNA for <i>in Vivo</i> Gene Delivery"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285708189375232","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Quantized Folding of Plasmid DNA Condensed with Block Catiomer into Characteristic Rod Structures Promoting Transgene Efficacy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285713114574720","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"siRNA-Based Therapy Ameliorates Glomerulonephritis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360286989762680448","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Scavenger Receptor A‐Mediated Targeting of Carboxylated Polyrotaxanes to Macrophages and the Impacts of Supramolecular Structure"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565165954017024","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Polyplex nanomicelle promotes hydrodynamic gene introduction to skeletal muscle"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565166827352320","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Rational design of smart supramolecular assemblies for gene delivery: chemical challenges in the creation of artificial viruses"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567181960556416","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Induced packaging of mRNA into polyplex micelles by regulated hybridization with a small number of cholesteryl RNA oligonucleotides directed enhanced in vivo transfection"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567189466249600","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Development of Biodegradable Polycation-Based Inhalable Dry Gene Powders by Spray Freeze Drying"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846640757192576","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effect of shear stress on structure and function of polyplex micelles from poly(ethylene glycol)-poly(l-lysine) block copolymers as systemic gene delivery carrier"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848656936984320","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Optimized rod length of polyplex micelles for maximizing transfection efficiency and their performance in systemic gene therapy against stroma-rich pancreatic tumors"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848658175521664","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Antiangiogenic Gene Therapy of Solid Tumor by Systemic Injection of Polyplex Micelles Loading Plasmid DNA Encoding Soluble Flt-1"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848658347218304","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Intratracheal Gene Transfer of Adrenomedullin Using Polyplex Nanomicelles Attenuates Monocrotaline-induced Pulmonary Hypertension in Rats"}]},{"@id":"https://cir.nii.ac.jp/crid/1360857593661867520","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Bridging mRNA and Polycation Using RNA Oligonucleotide Derivatives Improves the Robustness of Polyplex Micelles for Efficient mRNA Delivery"}]},{"@id":"https://cir.nii.ac.jp/crid/1360869855588575232","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effective design of PEGylated polyion complex (PIC) nanoparticles for enhancing PIC internalisation in cells utilising block copolymer combinations with mismatched ionic chain lengths"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204482993792","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Design of Polymeric Micelles as Nano-Biodevices"},{"@language":"ja","@value":"ナノバイオデバイスとして機能する高分子ミセル設計"},{"@language":"ja-Kana","@value":"ナノバイオデバイス ト シテ キノウスル コウブンシ ミセル セッケイ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204631264384","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Development of New Formulation Dry Powder for Pulmonary Delivery Using Amino Acids to Improve Stability"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679609272960","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Study on Development of Polymeric Micellar Gene Carrier and Evaluation of Its Functionality"}]},{"@id":"https://cir.nii.ac.jp/crid/1520009407430510080","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"超分子集合体を基盤とした遺伝子キャリアの構築と遺伝子導入効果"},{"@language":"ja-Kana","@value":"チョウブンシ シュウゴウタイ オ キバン ト シタ イデンシ キャリア ノ コウチク ト イデンシ ドウニュウ コウカ"}]},{"@id":"https://cir.nii.ac.jp/crid/1520290883170877056","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Ultrasound-Mediated Gene Transfection In vitro : Enhanced Efficiency by Complexation of Plasmid DNA"}]},{"@id":"https://cir.nii.ac.jp/crid/1520290884223964672","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"インテリジェント高分子材料による標的治療型ドラッグデリバリーシステム"},{"@language":"ja-Kana","@value":"インテリジェント コウブンシ ザイリョウ ニ ヨル ヒョウテキ チリョウガタ ドラッグデリバリー システム"}]},{"@id":"https://cir.nii.ac.jp/crid/1520853832705750272","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"生命システムとバイオ・メディカルテクノロジーにおける熱・物質移動(続)高分子ナノミセルによる薬物・遺伝子のピンポイントデリバリー"},{"@language":"ja-Kana","@value":"セイメイ システム ト バイオ メディカルテクノロジー ニ オケル ネツ ブッシツ イドウ ゾク コウブンシ ナノ ミセル ニ ヨル ヤクブツ イデンシ ノ ピンポイントデリバリー"}]},{"@id":"https://cir.nii.ac.jp/crid/2051151842055755904","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Molecular design of polymer-based carriers for plasmid DNA delivery in vitro and in vivo"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/s0142-9612(03)00347-8"},{"@type":"CIA","@value":"30003052738"},{"@type":"OPENAIRE","@value":"doi_dedup___::2d8bcf030326e684f0ab57a8b7d882dc"},{"@type":"CROSSREF","@value":"10.1002/anie.201608873_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1038/gt.2011.74_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1039/c5bm00081e_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1080/1061186x.2018.1550646_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1248/bpb.b13-00287_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1016/j.jconrel.2011.02.011_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1038/mt.2009.63_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1021/ja102739b_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1016/j.jconrel.2010.02.002_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1002/pi.5967_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1681/asn.2009030295_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1002/mabi.201800059_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1021/mp9002317_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1016/j.biomaterials.2011.09.046_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1016/j.jconrel.2009.12.014_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1039/c1cs15258k_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.3390/pharmaceutics7030233_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.7567/jjap.51.07gf29_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1016/j.biomaterials.2017.02.012_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.5650/oleoscience.7.111_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1021/bm401902j_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1016/j.biomaterials.2014.05.041_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1016/j.jconrel.2011.06.040_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1246/cl.190696_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1002/adhm.202102016_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1143/jjap.51.07gf29_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1039/d3tb02049e_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1016/j.reactfunctpolym.2010.10.009_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1016/j.jconrel.2012.03.021_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1016/j.biomaterials.2019.01.023_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1016/j.biomaterials.2014.03.037_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1016/j.ijpharm.2011.10.044_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1016/j.jbiotec.2014.01.029_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1016/j.jconrel.2011.04.026_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"},{"@type":"CROSSREF","@value":"10.1248/bpb.b15-00822_references_DOI_RVDz5tJuiMZsyhNtzdy1M9Z6PQJ"}]}