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- Yu Kang
- Kunming University of Science and Technology 1 Faculty of Environmental Science and Engineering , , Kunming, Yunnan 650500 , China
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- Chu Chu
- Institute of Primate Translational Medicine, Kunming University of Science and Technology 2 , Kunming, Yunnan 650500 , China
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- Fang Wang
- Institute of Primate Translational Medicine, Kunming University of Science and Technology 2 , Kunming, Yunnan 650500 , China
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- Yuyu Niu
- Institute of Primate Translational Medicine, Kunming University of Science and Technology 2 , Kunming, Yunnan 650500 , China
説明
<jats:title>ABSTRACT</jats:title> <jats:p>Owing to their high similarity to humans, non-human primates (NHPs) provide an exceedingly suitable model for the study of human disease. In this Review, we summarize the history of transgenic NHP models and the progress of CRISPR/Cas9-mediated genome editing in NHPs, from the first proof-of-principle green fluorescent protein-expressing monkeys to sophisticated NHP models of human neurodegenerative disease that accurately phenocopy several complex disease features. We discuss not only the breakthroughs and advantages, but also the potential shortcomings of the application of the CRISPR/Cas9 system to NHPs that have emerged from the expanded understanding of this technology in recent years. Although off-target and mosaic mutations are the main concerns in CRISPR/Cas9-mediated NHP modeling, recent progress in genome editing techniques make it likely that these technical limitations will be overcome soon, bringing excellent prospects to human disease studies.</jats:p>
収録刊行物
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- Disease Models & Mechanisms
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Disease Models & Mechanisms 12 (10), dmm039982-, 2019-10-01
The Company of Biologists