{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699995870801792.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.bbrc.2010.03.110"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0006291X10005747?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0006291X10005747?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"20331971"}}],"dc:title":[{"@value":"Functional availability of γ-herpesvirus K-cyclin is regulated by cellular CDK6 and p16INK4a"}],"description":[{"notation":[{"@value":"Viral K-cyclin derived from Kaposi's sarcoma-associated herpesvirus is homologous with mammalian D-type cyclins. Here, we demonstrated the regulatory mechanisms for K-cyclin function and degradation in human embryonic kidney HEK293 and primary effusion lymphoma JSC-1 cell lines. Proteasome inhibitor MG132 treatment induced an accumulation of ubiquitinated K-cyclin in these cells, and co-expression of CDK6 prevented K-cyclin ubiquitination. Also K-cyclin mutants incompetent for CDK6-binding were destabilized by proteasome pathway. Furthermore, silencing of p16INK4a promoted K-cyclin-CDK6 complex formation and hence induced K-cyclin-associated kinase activity in HEK293 cells. These observations indicate that CDK6-bound K-cyclin is functionally stable but monomeric K-cyclin is targeted to ubiquitin-dependent degradation pathway in these cells. Our data suggest that the balance between CDK6 and p16INK4a regulates the availability of functional K-cyclin in human cells."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699995870801798","@type":"Researcher","foaf:name":[{"@value":"Hidenori Yoshioka"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995870801793","@type":"Researcher","foaf:name":[{"@value":"Kohji Noguchi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995870801796","@type":"Researcher","foaf:name":[{"@value":"Kazuhiro Katayama"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995870801797","@type":"Researcher","foaf:name":[{"@value":"Junko Mitsuhashi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995870801792","@type":"Researcher","foaf:name":[{"@value":"Satoshi Yamagoe"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995870801794","@type":"Researcher","foaf:name":[{"@value":"Masahiro Fujimuro"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995870801795","@type":"Researcher","foaf:name":[{"@value":"Yoshikazu Sugimoto"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0006291X"}],"prism:publicationName":[{"@value":"Biochemical and Biophysical Research Communications"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2010-04","prism:volume":"394","prism:number":"4","prism:startingPage":"1000","prism:endingPage":"1005"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0006291X10005747?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0006291X10005747?httpAccept=text/plain"}],"createdAt":"2010-03-22","modifiedAt":"2020-06-03","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Herpesvirus%204,%20Human","dc:title":"Herpesvirus 4, Human"},{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Stability","dc:title":"Protein Stability"},{"@id":"https://cir.nii.ac.jp/all?q=Ubiquitination","dc:title":"Ubiquitination"},{"@id":"https://cir.nii.ac.jp/all?q=Cyclin-Dependent%20Kinase%206","dc:title":"Cyclin-Dependent Kinase 6"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line","dc:title":"Cell Line"},{"@id":"https://cir.nii.ac.jp/all?q=Viral%20Proteins","dc:title":"Viral Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Cyclins","dc:title":"Cyclins"},{"@id":"https://cir.nii.ac.jp/all?q=Herpesvirus%208,%20Human","dc:title":"Herpesvirus 8, Human"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Silencing","dc:title":"Gene Silencing"},{"@id":"https://cir.nii.ac.jp/all?q=Sarcoma,%20Kaposi","dc:title":"Sarcoma, Kaposi"},{"@id":"https://cir.nii.ac.jp/all?q=Cyclin-Dependent%20Kinase%20Inhibitor%20p16","dc:title":"Cyclin-Dependent Kinase Inhibitor p16"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360283693122892160","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effect of <scp>AKT</scp>3 expression on <scp>MYC</scp>‐ and caspase‐8‐dependent apoptosis caused by polo‐like kinase inhibitors in <scp>HCT</scp> 116 cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848656930487296","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Novel regulatory role for Kaposi’s sarcoma-associated herpesvirus-encoded vFLIP in chemosensitization to bleomycin"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/j.bbrc.2010.03.110"},{"@type":"OPENAIRE","@value":"doi_dedup___::a88861bac977e71827f58352e4a22a85"},{"@type":"CROSSREF","@value":"10.1111/cas.13093_references_DOI_MrO2dM5DeimPjuxpbAkN7gCrwRz"},{"@type":"CROSSREF","@value":"10.1016/j.bbrc.2011.10.050_references_DOI_MrO2dM5DeimPjuxpbAkN7gCrwRz"}]}