{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699995935246464.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/rcm.5263"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.5263"}},{"identifier":{"@type":"URI","@value":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdf/10.1002/rcm.5263"}},{"identifier":{"@type":"PMID","@value":"22095504"}}],"dc:title":[{"@value":"Mammalian DNA δ\n                    <sup>15</sup>\n                    N exhibits 40‰ intramolecular variation and is unresponsive to dietary protein level"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>\n                    We report the first high‐precision characterization of molecular and intramolecular δ\n                    <jats:sup>15</jats:sup>\n                    N of nucleosides derived from mammalian DNA. The influence of dietary protein level on brain amino acids and deoxyribonucleosides was determined to investigate whether high protein turnover would alter amino acid\n                    <jats:sup>15</jats:sup>\n                     N or\n                    <jats:sup>13</jats:sup>\n                     C values. Pregnant guinea pig dams were fed control diets, or high or low levels of dietary protein throughout gestation, and all pups were fed control diets. The cerebellar DNA of offspring was extracted at 2 and 120 days of life, nucleosides isolated and δ\n                    <jats:sup>15</jats:sup>\n                    N and δ\n                    <jats:sup>13</jats:sup>\n                    C values characterized. Mean diet δ\n                    <jats:sup>15</jats:sup>\n                    N was 0.45 ± 0.33‰, compared with cerebellar whole tissue and DNA δ\n                    <jats:sup>15</jats:sup>\n                    N = +4.1 ± 0.7‰ and −4.5 ± 0.4‰, respectively. Cerebellar deoxythymidine (dT), deoxycytidine (dC), deoxyadenosine (dA), and deoxyguanosine (dG) δ\n                    <jats:sup>15</jats:sup>\n                    N were +1.4 ± 0.4, –2.1 ± 0.9, –7.2 ± 0.3, and −10.4 ± 0.5‰, respectively. There were no changes in amino acid or deoxyribonucleoside δ\n                    <jats:sup>15</jats:sup>\n                    N values due to dietary protein level. Using known metabolic relationships, we developed equations to calculate the intramolecular δ\n                    <jats:sup>15</jats:sup>\n                    N values originating from aspartate (asp) in purines (pur) or pyrimidines (pyr), glutamine (glu), and glycine (gly) to be δ\n                    <jats:sup>15</jats:sup>\n                    N\n                    <jats:sub>ASP‐PUR</jats:sub>\n                    , δ\n                    <jats:sup>15</jats:sup>\n                    N\n                    <jats:sub>ASP‐PYR</jats:sub>\n                    , δ\n                    <jats:sup>15</jats:sup>\n                    N\n                    <jats:sub>GLN</jats:sub>\n                    , and δ\n                    <jats:sup>15</jats:sup>\n                    N\n                    <jats:sub>GLY</jats:sub>\n                    +11.9 ± 2.3‰, +7.0 ± 2.0‰, –9.1 ± 2.4‰, and −31.8 ± 8.9‰, respectively. A subset of twelve amino acids from food and brain had mean δ\n                    <jats:sup>15</jats:sup>\n                    N values of 4.3 ± 3.2‰ and 13.8 ± 3.1‰, respectively, and δ\n                    <jats:sup>15</jats:sup>\n                    N values for gly and asp were 12.6 ± 2.2‰ and 15.2 ± 0.8‰, respectively. A separate isotope tracer study detected no significant turnover of cerebellar DNA in the first six months of life. The large negative δ\n                    <jats:sup>15</jats:sup>\n                    N difference between gly and cerebellar purine N at the gly (7) position implies either that there is a major isotope effect during DNA synthesis, or that\n                    <jats:italic>in utero</jats:italic>\n                    gly has a different isotope ratio during rapid growth and metabolism from that in adult life. Our data show that cerebellar nucleoside intramolecular δ\n                    <jats:sup>15</jats:sup>\n                    N values vary over more than 40‰ and are not influenced by dietary protein level or age. Copyright © 2011 John Wiley & Sons, Ltd.\n                  </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699995935246467","@type":"Researcher","foaf:name":[{"@value":"Maggie S. Strable"}],"jpcoar:affiliationName":[{"@value":"Cornell University Division of Nutritional Sciences, Savage Hall  Ithaca NY 14853 USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995935246469","@type":"Researcher","foaf:name":[{"@value":"Carolyn L. Tschanz"}],"jpcoar:affiliationName":[{"@value":"Cornell University Division of Nutritional Sciences, Savage Hall  Ithaca NY 14853 USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995935246468","@type":"Researcher","foaf:name":[{"@value":"Behzad Varamini"}],"jpcoar:affiliationName":[{"@value":"Cornell University Division of Nutritional Sciences, Savage Hall  Ithaca NY 14853 USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995935246466","@type":"Researcher","foaf:name":[{"@value":"Yoshito Chikaraishi"}],"jpcoar:affiliationName":[{"@value":"Institute of Biogeosciences Japan Agency for Marine‐Earth Science and Technology  2‐15 Natsushima‐cho Yokosuka 237‐0061 Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995935246465","@type":"Researcher","foaf:name":[{"@value":"Naohiko Ohkouchi"}],"jpcoar:affiliationName":[{"@value":"Institute of Biogeosciences Japan Agency for Marine‐Earth Science and Technology  2‐15 Natsushima‐cho Yokosuka 237‐0061 Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995935246464","@type":"Researcher","foaf:name":[{"@value":"J. Thomas Brenna"}],"jpcoar:affiliationName":[{"@value":"Cornell University Division of Nutritional Sciences, Savage Hall  Ithaca NY 14853 USA"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09514198"},{"@type":"EISSN","@value":"10970231"}],"prism:publicationName":[{"@value":"Rapid Communications in Mass Spectrometry"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2011-11-04","prism:volume":"25","prism:number":"23","prism:startingPage":"3555","prism:endingPage":"3562"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.5263"},{"@id":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdf/10.1002/rcm.5263"}],"createdAt":"2011-11-08","modifiedAt":"2025-10-26","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=Analysis%20of%20Variance","dc:title":"Analysis of Variance"},{"@id":"https://cir.nii.ac.jp/all?q=Nitrogen%20Isotopes","dc:title":"Nitrogen Isotopes"},{"@id":"https://cir.nii.ac.jp/all?q=Guinea%20Pigs","dc:title":"Guinea Pigs"},{"@id":"https://cir.nii.ac.jp/all?q=DNA","dc:title":"DNA"},{"@id":"https://cir.nii.ac.jp/all?q=Mass%20Spectrometry","dc:title":"Mass Spectrometry"},{"@id":"https://cir.nii.ac.jp/all?q=Liver","dc:title":"Liver"},{"@id":"https://cir.nii.ac.jp/all?q=Pregnancy","dc:title":"Pregnancy"},{"@id":"https://cir.nii.ac.jp/all?q=Cerebellum","dc:title":"Cerebellum"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Animal%20Nutritional%20Physiological%20Phenomena","dc:title":"Animal Nutritional Physiological Phenomena"},{"@id":"https://cir.nii.ac.jp/all?q=Female","dc:title":"Female"},{"@id":"https://cir.nii.ac.jp/all?q=Dietary%20Proteins","dc:title":"Dietary Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Amino%20Acids","dc:title":"Amino Acids"},{"@id":"https://cir.nii.ac.jp/all?q=Radioactive%20Tracers","dc:title":"Radioactive Tracers"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004231875437184","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Organic Nitrogen: Sources, Fates, and Chemistry"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021391857928064","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Abundant extraterrestrial purine nucleobases in the Murchison meteorite: Implications for a unified mechanism for purine synthesis in carbonaceous chondrite parent bodies"}]},{"@id":"https://cir.nii.ac.jp/crid/1360306906071433344","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Compound-Specific Carbon and Nitrogen Isotopic Analyses of Underivatized Pyrimidine and Purine Nucleobases"}]},{"@id":"https://cir.nii.ac.jp/crid/1390014580469602304","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"A new era of isotope ecology: Nitrogen isotope ratio of amino acids as an approach for unraveling modern and ancient food web"}]},{"@id":"https://cir.nii.ac.jp/crid/2050870367118332288","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Biochemical and physiological bases for the use of carbon and nitrogen isotopes in environmental and ecological studies"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1002/rcm.5263"},{"@type":"OPENAIRE","@value":"doi_dedup___::378e51057ec48271316df7a27ceab73f"},{"@type":"CROSSREF","@value":"10.1016/j.gca.2023.10.024_references_DOI_DvS8gEGe4ZagdQmG9rFDKWZCIGL"},{"@type":"CROSSREF","@value":"10.1021/acsearthspacechem.4c00110_references_DOI_DvS8gEGe4ZagdQmG9rFDKWZCIGL"},{"@type":"CROSSREF","@value":"10.1186/s40645-015-0032-y_references_DOI_DvS8gEGe4ZagdQmG9rFDKWZCIGL"},{"@type":"CROSSREF","@value":"10.2183/pjab.99.009_references_DOI_DvS8gEGe4ZagdQmG9rFDKWZCIGL"},{"@type":"CROSSREF","@value":"10.1016/b978-0-08-095975-7.01015-9_references_DOI_DvS8gEGe4ZagdQmG9rFDKWZCIGL"}]}