{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699995977697024.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/aac.43.6.1449"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/AAC.43.6.1449"}},{"identifier":{"@type":"PMID","@value":"10348769"}}],"dc:title":[{"@value":"Cloning and Nucleotide Sequence Determination of the Entire\n            <i>mec</i>\n            DNA of Pre-Methicillin-Resistant\n            <i>Staphylococcus aureus</i>\n            N315"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            In methicillin-resistant\n            <jats:italic>Staphylococcus aureus</jats:italic>\n            , the methicillin resistance gene\n            <jats:italic>mecA</jats:italic>\n            is localized within a large chromosomal region which is absent in the methicillin-susceptible\n            <jats:italic>S. aureus</jats:italic>\n            chromosome. The region, designated\n            <jats:italic>mec</jats:italic>\n            DNA, is speculated to have originated from the genome of another bacterial species and become integrated into the chromosome of the\n            <jats:italic>S. aureus</jats:italic>\n            cell in the past. We report here cloning and determination of the structure of the entire\n            <jats:italic>mec</jats:italic>\n            DNA sequence from a Japanese\n            <jats:italic>S. aureus</jats:italic>\n            strain, N315. The\n            <jats:italic>mec</jats:italic>\n            DNA was found to be 51,669 bp long, including terminal inverted repeats of 27 bp and a characteristic pair of direct repeat sequences of 15 bp each: one is situated in the right extremity of\n            <jats:italic>mec</jats:italic>\n            DNA, and the other is situated outside the\n            <jats:italic>mec</jats:italic>\n            DNA and abuts the left boundary of\n            <jats:italic>mec</jats:italic>\n            DNA. The integration site of\n            <jats:italic>mec</jats:italic>\n            DNA was found to be located in an open reading frame (ORF) of unknown function, designated\n            <jats:italic>orfX</jats:italic>\n            . Clusters of antibiotic resistance genes were noted in\n            <jats:italic>mec</jats:italic>\n            DNA carried by transposon Tn\n            <jats:italic>554</jats:italic>\n            and an integrated copy of plasmid pUB110. Both the transposon and plasmid were integrated in the proximity of the\n            <jats:italic>mecA</jats:italic>\n            gene, the latter being flanked by a pair of insertion sequence IS\n            <jats:italic>431</jats:italic>\n            elements. Many ORFs other than those encoding antibiotic resistance were considered nonfunctional because of the acquired mutations or partial deletions found in the ORFs. Two ORFs potentially encoding novel site-specific recombinases were found in\n            <jats:italic>mec</jats:italic>\n            DNA. However, there was no ORF that might encode\n            <jats:italic>mec</jats:italic>\n            DNA-specific transposase or integrase proteins, indicating that the\n            <jats:italic>mec</jats:italic>\n            DNA is not a transposon or a bacteriophage in nature.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004230492439427","@type":"Researcher","foaf:name":[{"@value":"T. Ito"}],"jpcoar:affiliationName":[{"@value":"<!--label omitted: 1-->Department of Bacteriology, Juntendo University, Tokyo, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995977697026","@type":"Researcher","foaf:name":[{"@value":"Y. Katayama"}],"jpcoar:affiliationName":[{"@value":"<!--label omitted: 1-->Department of Bacteriology, Juntendo University, Tokyo, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699995977697025","@type":"Researcher","foaf:name":[{"@value":"K. Hiramatsu"}],"jpcoar:affiliationName":[{"@value":"<!--label omitted: 1-->Department of Bacteriology, Juntendo University, Tokyo, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00664804"},{"@type":"EISSN","@value":"10986596"}],"prism:publicationName":[{"@value":"Antimicrobial Agents and Chemotherapy"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"1999-06","prism:volume":"43","prism:number":"6","prism:startingPage":"1449","prism:endingPage":"1458"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/AAC.43.6.1449"}],"createdAt":"2018-10-09","modifiedAt":"2022-02-21","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=DNA,%20Bacterial","dc:title":"DNA, Bacterial"},{"@id":"https://cir.nii.ac.jp/all?q=Base%20Composition","dc:title":"Base Composition"},{"@id":"https://cir.nii.ac.jp/all?q=Staphylococcus%20aureus","dc:title":"Staphylococcus aureus"},{"@id":"https://cir.nii.ac.jp/all?q=Base%20Sequence","dc:title":"Base Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Sequence%20Data","dc:title":"Molecular Sequence Data"},{"@id":"https://cir.nii.ac.jp/all?q=Chromosomes,%20Bacterial","dc:title":"Chromosomes, Bacterial"},{"@id":"https://cir.nii.ac.jp/all?q=Open%20Reading%20Frames","dc:title":"Open Reading Frames"},{"@id":"https://cir.nii.ac.jp/all?q=Methicillin%20Resistance","dc:title":"Methicillin Resistance"},{"@id":"https://cir.nii.ac.jp/all?q=Amino%20Acid%20Sequence","dc:title":"Amino Acid Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Cloning,%20Molecular","dc:title":"Cloning, Molecular"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050001339199059584","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Fitness of Spontaneous Rifampicin-Resistant Staphylococcus aureus Isolates in a Biofilm Environment"}]},{"@id":"https://cir.nii.ac.jp/crid/1050564287442187520","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Genomic Basis for Methicillin Resistance in Staphylococcus aureus"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002215832860800","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Anti-MRSA activity of isoplagiochin-type macrocyclic bis(bibenzyl)s is mediated through cell membrane damage"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004230492439168","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283690810744448","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Minimum structural requirements for cell membrane leakage-mediated anti-MRSA activity of macrocyclic bis(bibenzyl)s"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567181970270080","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Structure–anti-MRSA activity relationship of macrocyclic bis(bibenzyl) derivatives"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846640947525632","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Multi-drug-resistant Staphylococcus aureus and future chemotherapy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360853567698185344","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Epidemiology of methicillin‐resistant <i>Staphylococcus aureus</i> in a Japanese neonatal intensive care unit"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233269503923712","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Identification of a Novel Gene Associated with High-Level β-Lactam Resistance in Heterogeneous Vancomycin-Intermediate Staphylococcus aureus Strain Mu3 and Methicillin-Resistant S. aureus Strain N315"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205519850112","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Future Chemotherapy Preventing Emergence of Multi-Antibiotic Resistance"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679434855168","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effect of Histamine-producing Bacteria on Fermented Fishery Products"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680723314816","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"THE MUTATION OF <i>rpoB</i> GENE ENCODING BETA-SUBUNIT OF RNA POLYMERASE IS ASSOCIATED WITH IMPROVEMENT OF LINEZOLID SUSCEPTIBILITY IN <i>Staphylococcus aureus</i>"},{"@language":"ja","@value":"黄色ブドウ球菌におけるRNAポリメラーゼ遺伝子の突然変異はLinezolidの高感受性化に関連する"},{"@language":"ja-Kana","@value":"オウショクブドウキュウキン ニ オケル RNA ポリメラーゼ イデンシ ノ トツゼン ヘンイ ワ Linezolid ノ コウカンジュセイカ ニ カンレン 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