{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699996038127616.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/s0027-5107(99)00029-9"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0027510799000299?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0027510799000299?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"10082921"}}],"dc:title":[{"@value":"Mechanism of oxidative DNA damage induced by quercetin in the presence of Cu(II)"}],"description":[{"notation":[{"@value":"Quercetin, one of flavonoids, has been reported to be carcinogenic. There have been no report concerning carcinogenicity of kaempferol and luteolin which have structure similar to quercetin. DNA damage was examined by using DNA fragments obtained from the human p53 tumor suppressor gene. Quercetin induced extensive DNA damage via reacting with Cu(II), but kaempferol and luteolin induced little DNA damage even in the presence of Cu(II). Excessive quercetin inhibited copper-dependent DNA damage induced by quercetin. Bathocuproine, a Cu(I)-specific chelator, catalase and methional inhibited the DNA damage by quercetin, whereas free hydroxyl radical scavengers did not. Site specificity of the DNA damage was thymine and cytosine residues. The site specificity and the inhibitory effects suggested that DNA-copper-oxygen complex rather than free hydroxyl radical induced the DNA damage. Formation of 8-oxodG by quercetin increased extensively in the presence of Cu(II), whereas 8-oxodG formation by kaempferol or luteolin increased only slightly. This study suggests a good relationship between carcinogenicity and oxidative DNA damage of three flavonoids. The mechanism of DNA damage by quercetin was discussed in relation to the safety in cancer chemoprevention by flavonoids."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699996038127617","@type":"Researcher","foaf:name":[{"@value":"Naruto Yamashita"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699996038127618","@type":"Researcher","foaf:name":[{"@value":"Hiroshi Tanemura"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699996038127616","@type":"Researcher","foaf:name":[{"@value":"Shosuke Kawanishi"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13861964"},{"@type":"PISSN","@value":"00275107"}],"prism:publicationName":[{"@value":"Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"1999-03","prism:volume":"425","prism:number":"1","prism:startingPage":"107","prism:endingPage":"115"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","https://www.elsevier.com/legal/tdmrep-license"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0027510799000299?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0027510799000299?httpAccept=text/plain"}],"createdAt":"2002-07-25","modifiedAt":"2025-12-03","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Hydrolysis","dc:title":"Hydrolysis"},{"@id":"https://cir.nii.ac.jp/all?q=Deoxyguanosine","dc:title":"Deoxyguanosine"},{"@id":"https://cir.nii.ac.jp/all?q=DNA","dc:title":"DNA"},{"@id":"https://cir.nii.ac.jp/all?q=Genes,%20p53","dc:title":"Genes, p53"},{"@id":"https://cir.nii.ac.jp/all?q=Mass%20Spectrometry","dc:title":"Mass Spectrometry"},{"@id":"https://cir.nii.ac.jp/all?q=Oxidative%20Stress","dc:title":"Oxidative Stress"},{"@id":"https://cir.nii.ac.jp/all?q=8-Hydroxy-2'-Deoxyguanosine","dc:title":"8-Hydroxy-2'-Deoxyguanosine"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Cattle","dc:title":"Cattle"},{"@id":"https://cir.nii.ac.jp/all?q=Quercetin","dc:title":"Quercetin"},{"@id":"https://cir.nii.ac.jp/all?q=Copper","dc:title":"Copper"},{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Damage","dc:title":"DNA Damage"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360009142901098368","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Polyphenols with Anti-Amyloid β Aggregation Show Potential Risk of Toxicity Via Pro-Oxidant Properties"}]},{"@id":"https://cir.nii.ac.jp/crid/1360584341811527936","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Myricetin causes site-specific DNA damage via reactive oxygen species generation by redox interactions with copper ions"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204575470592","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Superoxide Anion Generation via Electron-Transfer Oxidation of Catechin Dianion by Molecular Oxygen in an Aprotic Medium"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206473145472","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Protective Effects of Quercetin and Its Metabolites on H2O2-Induced Chromosomal Damage to WIL2-NS Cells"},{"@value":"Protective Effects of Quercetin and Its Metabolites on H<sub>2</sub>O<sub>2</sub>-Induced Chromosomal Damage to WIL2-NS Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679601108608","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Oxidation of Flavonoids which Promote DNA Degradation Induced by Bleomycin-Fe Complex"}]},{"@id":"https://cir.nii.ac.jp/crid/2050025942007635584","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Estrogen- and stress-induced DNA damage in breast cancer and chemoprevention with dietary flavonoid"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/s0027-5107(99)00029-9"},{"@type":"OPENAIRE","@value":"doi_dedup___::2a403fc2930a87b8308912a156065819"},{"@type":"CROSSREF","@value":"10.3390/ijms21103561_references_DOI_Lgz53UiWcRWOEHGuUFUg4XYxofF"},{"@type":"CROSSREF","@value":"10.1246/cl.2001.1152_references_DOI_Lgz53UiWcRWOEHGuUFUg4XYxofF"},{"@type":"CROSSREF","@value":"10.1016/j.mrgentox.2023.503694_references_DOI_Lgz53UiWcRWOEHGuUFUg4XYxofF"},{"@type":"CROSSREF","@value":"10.1186/s41021-016-0071-7_references_DOI_Lgz53UiWcRWOEHGuUFUg4XYxofF"},{"@type":"CROSSREF","@value":"10.1271/bbb.68.271_references_DOI_Lgz53UiWcRWOEHGuUFUg4XYxofF"},{"@type":"CROSSREF","@value":"10.1248/bpb.26.1108_references_DOI_Lgz53UiWcRWOEHGuUFUg4XYxofF"}]}