{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699996098607488.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.97.26.14789"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.97.26.14789"}},{"identifier":{"@type":"NAID","@value":"30016223394"}}],"dc:title":[{"@value":"Structure–function analysis of the tobacco mosaic virus resistance gene\n            <i>N</i>"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>\n            The tobacco\n            <jats:italic>N</jats:italic>\n            gene is a member of the\n Toll-interleukin-1 receptor/nucleotide-binding\n site/leucine-rich repeat (TIR-NBS-LRR) class of plant resistance\n (\n            <jats:italic>R</jats:italic>\n            ) genes and confers resistance to tobacco mosaic virus\n (TMV). We investigated the importance of specific domains of\n            <jats:italic>N</jats:italic>\n            in inducing TMV resistance, by examining various\n            <jats:italic>N</jats:italic>\n            deletion and point mutations that introduce single\n amino acid substitution mutants\n            <jats:italic>in vivo</jats:italic>\n            . Our deletion\n analysis suggests that the TIR, NBS, and LRR domains play an\n indispensable role in the induction of resistance responses against\n TMV. We show that amino acids conserved among the\n Toll/IL-1R/plant\n            <jats:italic>R</jats:italic>\n            gene TIR domain and\n NBS-containing proteins play a critical role in\n            <jats:italic>N</jats:italic>\n            -mediated TMV resistance. Some loss-of-function\n            <jats:italic>N</jats:italic>\n            alleles such as the TIR deletion and point mutations\n in the NBS (G216A/E/V/R, G218R, G219D,\n K222E/N, and T223A/N) interfere with the wild-type\n            <jats:italic>N</jats:italic>\n            function and behave like dominant negative mutations.\n These F\n            <jats:sub>1</jats:sub>\n            plants mount a hypersensitive response (HR) that\n is indistinguishable from that of the wild-type\n            <jats:italic>N</jats:italic>\n            plants, yet TMV was able to move systemically, causing a systemic\n hypersensitive response (SHR). Many amino acid substitutions in the\n TIR, NBS, and LRR domains of\n            <jats:italic>N</jats:italic>\n            lead to a partial\n loss-of-function phenotype. These mutant plants mount delayed HR\n compared with the wild-type\n            <jats:italic>N</jats:italic>\n            plants and fail to contain\n the virus to the infection site. In addition, some partial\n loss-of-function alleles (W82S/A, W141S/A,\n G218V/S, and G219V) interfere with the wild-type\n            <jats:italic>N</jats:italic>\n            function, leading to SHR. The partial loss-of-function\n and dominant negative mutant alleles described in this report will be\n useful in furthering our understanding of the TIR-NBS-LRR class of\n            <jats:italic>R</jats:italic>\n            genes.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1583950401157586944","@type":"Researcher","foaf:name":[{"@value":"S. P. Dinesh-Kumar"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular, Cellular, and Developmental Biology,\r OML451, P.O. Box 208104, Yale University, New Haven, CT 06520-8104;\r Department of Plant and Microbial Biology, University of\r California, Berkeley, CA 94720; and Plant Gene\r Expression Center, U.S. Department of Agriculture,\r Albany, CA 94710"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857701671530496","@type":"Researcher","foaf:name":[{"@value":"Wai-Hong Tham"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular, Cellular, and Developmental Biology,\r OML451, P.O. Box 208104, Yale University, New Haven, CT 06520-8104;\r Department of Plant and Microbial Biology, University of\r California, Berkeley, CA 94720; and Plant Gene\r Expression Center, U.S. Department of Agriculture,\r Albany, CA 94710"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857701671530497","@type":"Researcher","foaf:name":[{"@value":"Barbara J. Baker"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular, Cellular, and Developmental Biology,\r OML451, P.O. Box 208104, Yale University, New Haven, CT 06520-8104;\r Department of Plant and Microbial Biology, University of\r California, Berkeley, CA 94720; and Plant Gene\r Expression Center, U.S. Department of Agriculture,\r Albany, CA 94710"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00278424"},{"@type":"EISSN","@value":"10916490"}],"prism:publicationName":[{"@value":"Proceedings of the National Academy of Sciences"}],"dc:publisher":[{"@value":"Proceedings of the National Academy of Sciences"}],"prism:publicationDate":"2000-12-19","prism:volume":"97","prism:number":"26","prism:startingPage":"14789","prism:endingPage":"14794"},"reviewed":"false","url":[{"@id":"https://pnas.org/doi/pdf/10.1073/pnas.97.26.14789"}],"createdAt":"2002-07-26","modifiedAt":"2022-04-13","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050025031481803776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Screening and analysis of genes expressed upon infection of broad bean with Clover yellow vein virus causing lethal necrosis"}]},{"@id":"https://cir.nii.ac.jp/crid/1050306506460613120","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Activation of the Salicylic Acid Signaling Pathway Enhances Clover yellow vein virus Virulence in Susceptible Pea Cultivars"},{"@value":"Activation of the Salicylic Acid Signaling Pathway Enhances <i>Clover yellow vein virus</i> Virulence in Susceptible Pea Cultivars"}]},{"@id":"https://cir.nii.ac.jp/crid/1050587981434483328","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Combinations of a host resistance gene and the CI gene of turnip mosaic virus differentially regulate symptom expression in Brassica rapa cultivars"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002217846593152","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Large-Scale Comparative Phosphoproteomics Identifies Conserved Phosphorylation Sites in Plants"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002219688718848","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The\n                    <scp>NB</scp>\n                    ‐\n                    <scp>LRR</scp>\n                    proteins\n                    <scp>RGA</scp>\n                    4 and\n                    <scp>RGA</scp>\n                    5 interact functionally and physically to confer disease resistance"}]},{"@id":"https://cir.nii.ac.jp/crid/1360009142756074240","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The rice NLR pair Pikp-1/Pikp-2 initiates cell death through receptor cooperation rather than negative regulation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013168774257152","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Resistance induction based on the understanding of molecular interactions between plant viruses and host plants"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285707426130304","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Increased cytosine methylation at promoter of the NB-LRR class R gene RCY1 correlated with compromised resistance to cucumber mosaic virus in EMS-generated src mutants of Arabidopsis thaliana"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848659708826112","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Cell Death Triggered by a Putative Amphipathic Helix of<i>Radish mosaic virus</i>Helicase Protein Is Tightly Correlated With Host Membrane Modification"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848660746948352","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Systemic necrosis in tomato induced by a Japanese isolate of rehmannia mosaic virus in a temperature‐sensitive manner"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679177010432","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"植物のオルガネラを介したプログラム細胞死"},{"@language":"en","@value":"Programmed Cell Death Mediated by Organelles in Plants"},{"@language":"ja-Kana","@value":"ショクブツ ノ オルガネラ オ カイシタ プログラム サイボウシ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681381819904","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"植物ウイルス抵抗性の打破：ウイルス抵抗性の永続性を予測したり延長させたりできるだろうか？"},{"@language":"en","@value":"Breakdown of plant virus resistance: can we predict and extend the durability of virus resistance?"},{"@language":"ja-Kana","@value":"ショクブツ ウイルス テイコウセイ ノ ダハ : ウイルス テイコウセイ ノ エイゾクセイ オ ヨソク シタリ エンチョウサセタリ デキル ダロウ カ?"},{"@value":"Breakdown of plant virus resistance: can we predict and extent the durability of virus resistance?"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1073/pnas.97.26.14789"},{"@type":"CIA","@value":"30016223394"},{"@type":"CROSSREF","@value":"10.15252/embj.201487923_references_DOI_NbbbXxucGBEePW0CCgpnF8R5lot"},{"@type":"CROSSREF","@value":"10.1371/journal.pone.0238616_references_DOI_NbbbXxucGBEePW0CCgpnF8R5lot"},{"@type":"CROSSREF","@value":"10.1186/s12985-021-01647-4_references_DOI_NbbbXxucGBEePW0CCgpnF8R5lot"},{"@type":"CROSSREF","@value":"10.1101/2020.06.20.162834_references_DOI_NbbbXxucGBEePW0CCgpnF8R5lot"},{"@type":"CROSSREF","@value":"10.1007/s10327-014-0527-1_references_DOI_NbbbXxucGBEePW0CCgpnF8R5lot"},{"@type":"CROSSREF","@value":"10.1104/pp.110.157347_references_DOI_NbbbXxucGBEePW0CCgpnF8R5lot"},{"@type":"CROSSREF","@value":"10.1007/s00705-011-1036-6_references_DOI_NbbbXxucGBEePW0CCgpnF8R5lot"},{"@type":"CROSSREF","@value":"10.1016/j.pmpp.2017.09.007_references_DOI_NbbbXxucGBEePW0CCgpnF8R5lot"},{"@type":"CROSSREF","@value":"10.1094/mpmi-01-15-0004-r_references_DOI_NbbbXxucGBEePW0CCgpnF8R5lot"},{"@type":"CROSSREF","@value":"10.1186/1743-422x-8-355_references_DOI_NbbbXxucGBEePW0CCgpnF8R5lot"},{"@type":"CROSSREF","@value":"10.1111/ppa.13006_references_DOI_NbbbXxucGBEePW0CCgpnF8R5lot"},{"@type":"CROSSREF","@value":"10.1094/mpmi-22-2-0166_references_DOI_NbbbXxucGBEePW0CCgpnF8R5lot"}]}