{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699996181855232.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1139/y01-011"}},{"identifier":{"@type":"URI","@value":"http://www.nrcresearchpress.com/doi/pdf/10.1139/y01-011"}}],"dc:title":[{"@value":"Estrogen effect on post-exercise skeletal muscle neutrophil infiltration and calpain activity"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>We hypothesized that estrogen administration would attenuate skeletal muscle neutrophil infiltration, indices of muscle membrane disruption, and muscle calpain activity shortly after the termination of exercise. Ovariectomized female rats were implanted with either an estogen pellet (25 mg β-estradiol) or a placebo pellet. Two weeks post-implant, animals were killed either at rest or 1 h after running exercise (60 min at 21 m·min<jats:sup>–1</jats:sup>, 12% grade). The 4 experimental groups (n = 12) used were: unexercised placebo (UP), unexercised estrogen (UE), exercised placebo (EP), and exercised estrogen (EE). Blood samples were analyzed for creatine kinase (CK) activity and estradiol content. Plantaris and gastrocnemius muscles were removed and histochemical determination of neutrophil content or biochemical determination of myeloperoxidase (MPO), glucose-6-phosphate dehydrogenase (G6PD), and calpain-like activity determined. Estrogen supplemented animals had 10–20-fold higher circulating estradiol levels than placebo animals. EP animals had significantly higher (P < 0.05) circulating CK activities than EE or unexercised animals. Muscle neutrophil concentrations were significantly (P < 0.01) elevated in EP and EE groups compared with unexercised controls, with EP muscle neutrophil levels also being over 60% greater (P < 0.05) than in EE animals. EP animals also had higher (P < 0.05) muscle MPO activities than unexercised or EE animals. Muscle G6PD activities were not significantly different between any groups. Muscle caplain-like activities were 80% higher (P < 0.01) in EP animals than EE animals with calpain-like activities in EE animals similar to unexercised groups. These results indicate that estrogen supplementation in ovariectomized rats attenuated 1-h post-exercise serum CK activities, muscle neutrophil infiltration, MPO activities, and calpain-like activities when compared with exercised, unsupplemented animals. This supports the possibility of a relationship between estrogen, calpain dependent production of neutrophil chemo-attractant peptides, and 1-h post-exercise skeletal muscle neutrophil infiltration.Key words: neutrophils, calpain, estrogen, skeletal muscle, muscle damage.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699996181855106","@type":"Researcher","foaf:name":[{"@value":"Peter M Tiidus"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699996181855107","@type":"Researcher","foaf:name":[{"@value":"Dean Holden"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699996181855108","@type":"Researcher","foaf:name":[{"@value":"Eric Bombardier"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699996181855105","@type":"Researcher","foaf:name":[{"@value":"Sheri Zajchowski"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699996181855109","@type":"Researcher","foaf:name":[{"@value":"Deborah Enns"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699996181855104","@type":"Researcher","foaf:name":[{"@value":"Angelo Belcastro"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00084212"},{"@type":"EISSN","@value":"12057541"}],"prism:publicationName":[{"@value":"Canadian Journal of Physiology and Pharmacology"}],"dc:publisher":[{"@value":"Canadian Science Publishing"}],"prism:publicationDate":"2001-05-01","prism:volume":"79","prism:number":"5","prism:startingPage":"400","prism:endingPage":"406"},"reviewed":"false","dc:rights":["http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining"],"url":[{"@id":"http://www.nrcresearchpress.com/doi/pdf/10.1139/y01-011"}],"createdAt":"2011-04-24","modifiedAt":"2021-11-22","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050852746519666560","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Estrogen Receptor β Controls Muscle Growth and Regeneration in Young Female Mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285711304246272","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Sex differences in intracellular Ca<sup>2+</sup> accumulation following eccentric contractions of rat skeletal muscle in vivo"}]},{"@id":"https://cir.nii.ac.jp/crid/1360286992048251520","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Functions of estrogen and estrogen receptor signaling on skeletal muscle"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565169649125504","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Estrogens maintain skeletal muscle and satellite cell functions"}]},{"@id":"https://cir.nii.ac.jp/crid/1360869454550325888","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Eccentric contraction increases hydrogen peroxide levels and alters gene expression through Nox2 in skeletal muscle of male mice"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1139/y01-011"},{"@type":"CROSSREF","@value":"10.1152/ajpregu.00623.2009_references_DOI_CuRgYKrPmX4OddCB5S1cS4xlE6a"},{"@type":"CROSSREF","@value":"10.1530/joe-15-0476_references_DOI_CuRgYKrPmX4OddCB5S1cS4xlE6a"},{"@type":"CROSSREF","@value":"10.1016/j.stemcr.2020.07.017_references_DOI_CuRgYKrPmX4OddCB5S1cS4xlE6a"},{"@type":"CROSSREF","@value":"10.1152/japplphysiol.00335.2024_references_DOI_CuRgYKrPmX4OddCB5S1cS4xlE6a"},{"@type":"CROSSREF","@value":"10.1016/j.jsbmb.2019.105375_references_DOI_CuRgYKrPmX4OddCB5S1cS4xlE6a"}]}