{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699996285216384.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/j.1365-2990.2008.00947.x"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2990.2008.00947.x"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2990.2008.00947.x"}},{"identifier":{"@type":"PMID","@value":"18482256"}}],"dc:title":[{"@value":"Protein microarray analysis identifies human cellular prion protein interactors"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p> <jats:bold>Aims:</jats:bold> To obtain an insight into the function of cellular prion protein (PrPC), we studied PrPC‐interacting proteins (PrPIPs) by analysing a protein microarray. <jats:bold>Methods:</jats:bold> We identified 47 novel PrPIPs by probing an array of 5000 human proteins with recombinant human PrPC spanning amino acid residues 23–231 named PR209. <jats:bold>Results:</jats:bold> The great majority of 47 PrPIPs were annotated as proteins involved in the recognition of nucleic acids. Coimmunoprecipitation and cell imaging in a transient expression system validated the interaction of PR209 with neuronal PrPIPs, such as FAM64A, HOXA1, PLK3 and MPG. However, the interaction did not generate proteinase K‐resistant proteins. KeyMolnet, a bioinformatics tool for analysing molecular interaction on the curated knowledge database, revealed that the complex molecular network of PrPC and PrPIPs has a significant relationship with AKT, JNK and MAPK signalling pathways. <jats:bold>Conclusions:</jats:bold> Protein microarray is a useful tool for systematic screening and comprehensive profiling of the human PrPC interactome. Because the network of PrPC and interactors involves signalling pathways essential for regulation of cell survival, differentiation, proliferation and apoptosis, these observations suggest a logical hypothesis that dysregulation of the PrPC interactome might induce extensive neurodegeneration in prion diseases.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380285529008378757","@type":"Researcher","foaf:name":[{"@value":"J. Satoh"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699996285216387","@type":"Researcher","foaf:name":[{"@value":"S. Obayashi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699996285216384","@type":"Researcher","foaf:name":[{"@value":"T. Misawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699996285216389","@type":"Researcher","foaf:name":[{"@value":"K. Sumiyoshi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699996285216385","@type":"Researcher","foaf:name":[{"@value":"K. Oosumi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699996285216386","@type":"Researcher","foaf:name":[{"@value":"H. Tabunoki"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03051846"},{"@type":"EISSN","@value":"13652990"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/03051846"}],"prism:publicationName":[{"@value":"Neuropathology and Applied Neurobiology"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2009-01-21","prism:volume":"35","prism:number":"1","prism:startingPage":"16","prism:endingPage":"35"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2990.2008.00947.x"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2990.2008.00947.x"}],"createdAt":"2008-05-08","modifiedAt":"2023-09-28","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Blotting,%20Western","dc:title":"Blotting, Western"},{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Array%20Analysis","dc:title":"Protein Array Analysis"},{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Serine-Threonine%20Kinases","dc:title":"Protein Serine-Threonine Kinases"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line","dc:title":"Cell Line"},{"@id":"https://cir.nii.ac.jp/all?q=Databases,%20Genetic","dc:title":"Databases, Genetic"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Immunoprecipitation","dc:title":"Immunoprecipitation"},{"@id":"https://cir.nii.ac.jp/all?q=PrPC%20Proteins","dc:title":"PrPC Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=RNA,%20Messenger","dc:title":"RNA, Messenger"},{"@id":"https://cir.nii.ac.jp/all?q=Neurons","dc:title":"Neurons"},{"@id":"https://cir.nii.ac.jp/all?q=Reverse%20Transcriptase%20Polymerase%20Chain%20Reaction","dc:title":"Reverse Transcriptase Polymerase Chain Reaction"},{"@id":"https://cir.nii.ac.jp/all?q=Tumor%20Suppressor%20Proteins","dc:title":"Tumor Suppressor Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Intracellular%20Signaling%20Peptides%20and%20Proteins","dc:title":"Intracellular Signaling Peptides and Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=JNK%20Mitogen-Activated%20Protein%20Kinases","dc:title":"JNK Mitogen-Activated Protein Kinases"},{"@id":"https://cir.nii.ac.jp/all?q=Nuclear%20Proteins","dc:title":"Nuclear Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Reproducibility%20of%20Results","dc:title":"Reproducibility of Results"},{"@id":"https://cir.nii.ac.jp/all?q=Endopeptidase%20K","dc:title":"Endopeptidase K"},{"@id":"https://cir.nii.ac.jp/all?q=Mitogen-Activated%20Protein%20Kinases","dc:title":"Mitogen-Activated Protein Kinases"},{"@id":"https://cir.nii.ac.jp/all?q=Carrier%20Proteins","dc:title":"Carrier Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Proto-Oncogene%20Proteins%20c-akt","dc:title":"Proto-Oncogene Proteins c-akt"},{"@id":"https://cir.nii.ac.jp/all?q=Signal%20Transduction","dc:title":"Signal Transduction"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002215370809984","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"TDP-43 Dimerizes in Human Cells in Culture"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567187570500736","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A 17-molecule set as a predictor of complete response to neoadjuvant chemotherapy with docetaxel, cisplatin, and 5-fluorouracil in esophageal cancer"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679627000192","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Molecular Network Analysis of Multiple Sclerosis Brain Lesion Proteome"},{"@language":"ja","@value":"多発性硬化症病変分子のネットワーク解析"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1111/j.1365-2990.2008.00947.x"},{"@type":"OPENAIRE","@value":"doi_dedup___::e84a7d8309b77994d9da6bad6314119d"},{"@type":"CROSSREF","@value":"10.1007/s10571-009-9489-9_references_DOI_6fnwuFHNXr6cRPjtUw5F82t3epu"},{"@type":"CROSSREF","@value":"10.1371/journal.pone.0188098_references_DOI_6fnwuFHNXr6cRPjtUw5F82t3epu"},{"@type":"CROSSREF","@value":"10.2177/jsci.33.182_references_DOI_6fnwuFHNXr6cRPjtUw5F82t3epu"}]}