{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699996447739648.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/s0093-691x(00)00297-1"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0093691X00002971?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0093691X00002971?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"10883843"}},{"identifier":{"@type":"NAID","@value":"30006153878"}}],"dc:title":[{"@value":"Fertilizability and developmental capacity of individually cultured bovine oocytes"}],"description":[{"notation":[{"@value":"Culture of single oocytes throughout in vitro maturation (IVM), fertilization (IVF) and culture (IVC) provides detailed information on maturity, fertilizability and developmental capacity of individual bovine oocytes and embryos. In the present study, effects of sperm concentration (Experiment 1), microdrop size (Experiment 2), and the addition of hypotaurine (HT) or glutathione (GSH; Experiment 3) during IVF were investigated. In Experiment 4, in vitro maturity and developmental capacity of bovine oocytes cultured for IVM in a medium supplemented with fetal calf serum (FCS), bovine serum albumin (BSA) or polyvinyl alcohol (PVA) during IVM were investigated. In Experiments 1 to 3, the percentages of normal (2 pronuclei with a spermtail) and polyspermic fertilization in singly cultured oocytes were similar to those of group IVF culture (5 oocytes/drop). The addition of GSH during single oocyte IVF significantly increased the proportion of normal fertilization and decreased the polyspermic fertilization compared with addition of HT or of the control. The rates of mature oocytes (62.4 and 67.7%) and blastocyst development (12.9 and 15.2%) for single oocyte IVM cultures (Experiment 4) were also similar compared with the group culture; PVA supplementation significantly increased the matured oocyte rate, but decreased blastocyst development significantly (7.1%) as compared with FCS (19.5%) or BSA (15.6%). These results indicate that a single oocyte culture system throughout in vitro production of bovine embryos provides similar maturity, fertilizability and developmental capacity to oocytes cultured in groups."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1582543026061285504","@type":"Researcher","foaf:name":[{"@value":"Y. Fukui"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699996447739650","@type":"Researcher","foaf:name":[{"@value":"Y. Kikuchi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699996447739648","@type":"Researcher","foaf:name":[{"@value":"H. Kondo"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699996447739651","@type":"Researcher","foaf:name":[{"@value":"S. Mizushima"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0093691X"}],"prism:publicationName":[{"@value":"Theriogenology"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2000-05","prism:volume":"53","prism:number":"8","prism:startingPage":"1553","prism:endingPage":"1565"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0093691X00002971?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0093691X00002971?httpAccept=text/plain"}],"createdAt":"2002-07-25","modifiedAt":"2019-05-02","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=Taurine","dc:title":"Taurine"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Culture%20Techniques","dc:title":"Cell Culture Techniques"},{"@id":"https://cir.nii.ac.jp/all?q=Serum%20Albumin,%20Bovine","dc:title":"Serum Albumin, Bovine"},{"@id":"https://cir.nii.ac.jp/all?q=Fertilization%20in%20Vitro","dc:title":"Fertilization in Vitro"},{"@id":"https://cir.nii.ac.jp/all?q=Glutathione","dc:title":"Glutathione"},{"@id":"https://cir.nii.ac.jp/all?q=Spermatozoa","dc:title":"Spermatozoa"},{"@id":"https://cir.nii.ac.jp/all?q=Culture%20Media","dc:title":"Culture Media"},{"@id":"https://cir.nii.ac.jp/all?q=Pregnancy","dc:title":"Pregnancy"},{"@id":"https://cir.nii.ac.jp/all?q=Polyvinyl%20Alcohol","dc:title":"Polyvinyl Alcohol"},{"@id":"https://cir.nii.ac.jp/all?q=Linear%20Models","dc:title":"Linear Models"},{"@id":"https://cir.nii.ac.jp/all?q=Oocytes","dc:title":"Oocytes"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Cattle","dc:title":"Cattle"},{"@id":"https://cir.nii.ac.jp/all?q=Female","dc:title":"Female"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1390001206335095040","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The Developmental Potential of Oocytes is Impaired in Cattle with Liver Abnormalities"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206337558528","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Oxygen Tension and Medium Supplements for In Vitro Maturation of Bovine Oocytes Cultured Individually in a Chemically Defined Medium"},{"@language":"ja","@value":"化学限定培地で個別培養されたウシ卵母細胞の体外成熟における酸素濃度と培養液への添加剤"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681313585536","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Modification of mitochondrial function, cytoplasmic lipid content and cryosensitivity of bovine embryos by resveratrol"},{"@value":"Modification of mitochondria function, cytoplasmic lipid content and cryosensitivity of bovine embryos by resveratrol"}]},{"@id":"https://cir.nii.ac.jp/crid/1571698599789858816","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"酸化ストレスが関わる成熟、受精、初期発生とその制御による効率的初期胚生産へのアプローチ"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/s0093-691x(00)00297-1"},{"@type":"CIA","@value":"30006153878"},{"@type":"OPENAIRE","@value":"doi_dedup___::9bbfdbe6e61ec15ee20510389edbf02d"},{"@type":"CROSSREF","@value":"10.1262/jrd.2012-163_references_DOI_LCv7hQIGBbq5iF0qghy7ZNffLkq"},{"@type":"CROSSREF","@value":"10.1262/jrd.2016-182_references_DOI_LCv7hQIGBbq5iF0qghy7ZNffLkq"},{"@type":"CROSSREF","@value":"10.1262/jrd.50.107_references_DOI_LCv7hQIGBbq5iF0qghy7ZNffLkq"}]}