{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361981468614390144.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.0402532101"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.0402532101"}},{"identifier":{"@type":"NAID","@value":"80016865425"}}],"dc:title":[{"@value":"Bioreactor-based bone tissue engineering: The influence of dynamic flow on osteoblast phenotypic expression and matrix mineralization"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>\n            An important issue in tissue engineering concerns the possibility of limited tissue ingrowth in tissue-engineered constructs because of insufficient nutrient transport. We report a dynamic flow culture system using high-aspect-ratio vessel rotating bioreactors and 3D scaffolds for culturing rat calvarial osteoblast cells. 3D scaffolds were designed by mixing lighter-than-water (density, <1g/ml) and heavier-than-water (density, >1g/ml) microspheres of 85:15 poly(lactide-\n            <jats:italic>co</jats:italic>\n            -glycolide). We quantified the rate of 3D flow through the scaffolds by using a particle-tracking system, and the results suggest that motion trajectories and, therefore, the flow velocity around and through scaffolds in rotating bioreactors can be manipulated by varying the ratio of heavier-than-water to lighter-than-water microspheres. When rat primary calvarial cells were cultured on the scaffolds in bioreactors for 7 days, the 3D dynamic flow environment affected bone cell distribution and enhanced cell phenotypic expression and mineralized matrix synthesis within tissue-engineered constructs compared with static conditions. These studies provide a foundation for exploring the effects of dynamic flow on osteoblast function and provide important insight into the design and optimization of 3D scaffolds suitable in bioreactors for\n            <jats:italic>in vitro</jats:italic>\n            tissue engineering of bone.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380013199402996353","@type":"Researcher","foaf:name":[{"@value":"Xiaojun Yu"}],"jpcoar:affiliationName":[{"@value":"Departments of Orthopaedic Surgery, Biomedical Engineering, and Chemical Engineering, University of Virginia, Charlottesville, VA 22903; The Wistar Institute, Philadelphia, PA 19104; and Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013199402996356","@type":"Researcher","foaf:name":[{"@value":"Edward A. Botchwey"}],"jpcoar:affiliationName":[{"@value":"Departments of Orthopaedic Surgery, Biomedical Engineering, and Chemical Engineering, University of Virginia, Charlottesville, VA 22903; The Wistar Institute, Philadelphia, PA 19104; and Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013199402996355","@type":"Researcher","foaf:name":[{"@value":"Elliot M. Levine"}],"jpcoar:affiliationName":[{"@value":"Departments of Orthopaedic Surgery, Biomedical Engineering, and Chemical Engineering, University of Virginia, Charlottesville, VA 22903; The Wistar Institute, Philadelphia, PA 19104; and Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013199402996352","@type":"Researcher","foaf:name":[{"@value":"Solomon R. Pollack"}],"jpcoar:affiliationName":[{"@value":"Departments of Orthopaedic Surgery, Biomedical Engineering, and Chemical Engineering, University of Virginia, Charlottesville, VA 22903; The Wistar Institute, Philadelphia, PA 19104; and Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013199402996354","@type":"Researcher","foaf:name":[{"@value":"Cato T. Laurencin"}],"jpcoar:affiliationName":[{"@value":"Departments of Orthopaedic Surgery, Biomedical Engineering, and Chemical Engineering, University of Virginia, Charlottesville, VA 22903; The Wistar Institute, Philadelphia, PA 19104; and Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00278424"},{"@type":"EISSN","@value":"10916490"}],"prism:publicationName":[{"@value":"Proceedings of the National Academy of Sciences"}],"dc:publisher":[{"@value":"Proceedings of the National Academy of Sciences"}],"prism:publicationDate":"2004-07-26","prism:volume":"101","prism:number":"31","prism:startingPage":"11203","prism:endingPage":"11208"},"reviewed":"false","url":[{"@id":"https://pnas.org/doi/pdf/10.1073/pnas.0402532101"}],"createdAt":"2004-07-27","modifiedAt":"2022-04-12","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004232221326592","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Synthesis of artificial lymphoid tissue with immunological function"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004234484386176","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Radial-Flow Bioreactor Enables Uniform Proliferation of Human Mesenchymal Stem Cells Throughout a Three-Dimensional Scaffold"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283690784068480","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effects of implantation of three-dimensional engineered bone tissue with a vascular-like structure on repair of bone defects"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283694136894976","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Bioreactor-based engineering of osteochondral grafts: from model systems to tissue manufacturing"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846644090306176","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Bioreactor design for tissue engineering"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204902983680","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"音声外科術後のvoice rest至適期間および音声治療効果について"},{"@language":"en","@value":"Voice Rest after Phonomicrosurgery"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679389976192","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Influence of Deacetylated Chitin 50-TYPE I Collagen Complex Gel to Bone Marrow-Derived Mesenchymal Cells"},{"@value":"脱アセチル化キチン‐ＴＹＰＥ　Ｉコラーゲン複合化ゲルの骨髄由来未分化間葉系細胞への影響"}]},{"@id":"https://cir.nii.ac.jp/crid/1521136279980537216","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"Bioreactor Design for Tissue Engineering"},{"@language":"ja-Kana","@value":"Bioreactor Design for Tissue Engineering"}]},{"@id":"https://cir.nii.ac.jp/crid/1522543655610857088","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"Bioreactor-Based Engineering of Osteochondral Grafts: from Model Systems to Tissue Manufacturing"},{"@language":"ja-Kana","@value":"Bioreactor Based Engineering of Osteochondral Grafts from Model Systems to Tissue Manufacturing"}]},{"@id":"https://cir.nii.ac.jp/crid/1523388080358433152","@type":"Article","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@value":"Application of computational fluid dynamics in tissue engineering"}]},{"@id":"https://cir.nii.ac.jp/crid/1571698600687303168","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"口腔アンチエイジングによる生体制御"}]},{"@id":"https://cir.nii.ac.jp/crid/1573105975509111936","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"PRPは凍結脂肪由来未分化間葉系幹細胞の増殖・分化を促進する"},{"@language":"en","@value":"PRP Promotes Proliferation and Differentiation of Cryopreserved Adipose-derived Mesenchymal Stem Cells AdSCs)"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1073/pnas.0402532101"},{"@type":"CIA","@value":"80016865425"},{"@type":"CROSSREF","@value":"10.5112/jjlp.57.193_references_DOI_3UU1NsWgrAl7by5TtLRVbtN7O3H"},{"@type":"CROSSREF","@value":"10.1263/jbb.100.235_references_DOI_3UU1NsWgrAl7by5TtLRVbtN7O3H"},{"@type":"CROSSREF","@value":"10.1016/j.it.2010.09.002_references_DOI_3UU1NsWgrAl7by5TtLRVbtN7O3H"},{"@type":"CROSSREF","@value":"10.1089/ten.tec.2011.0722_references_DOI_3UU1NsWgrAl7by5TtLRVbtN7O3H"},{"@type":"CROSSREF","@value":"10.1263/jbb.100.489_references_DOI_3UU1NsWgrAl7by5TtLRVbtN7O3H"},{"@type":"CROSSREF","@value":"10.1016/j.apsusc.2012.01.092_references_DOI_3UU1NsWgrAl7by5TtLRVbtN7O3H"},{"@type":"CROSSREF","@value":"10.1016/j.jbiosc.2012.03.010_references_DOI_3UU1NsWgrAl7by5TtLRVbtN7O3H"}]}