{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361981469073900416.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1091/mbc.e11-11-0925"}}],"dc:title":[{"@value":"Endosomal maturation by Rab conversion in <i>Aspergillus nidulans</i> is coupled to dynein-mediated basipetal movement"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p> We exploit the ease with which highly motile early endosomes are distinguished from static late endosomes in order to study Aspergillus nidulans endosomal traffic. RabS<jats:sup>Rab7</jats:sup> mediates homotypic fusion of late endosomes/vacuoles in a homotypic fusion- and vacuole protein sorting/Vps41–dependent manner. Progression across the endocytic pathway involves endosomal maturation because the end products of the pathway in the absence of RabS<jats:sup>Rab7</jats:sup> are minivacuoles that are competent in multivesicular body sorting and cargo degradation but retain early endosomal features, such as the ability to undergo long-distance movement and propensity to accumulate in the tip region if dynein function is impaired. Without RabS<jats:sup>Rab7</jats:sup>, early endosomal Rab5s—RabA and RabB—reach minivacuoles, in agreement with the view that Rab7 homologues facilitate the release of Rab5 homologues from endosomes. RabS<jats:sup>Rab7</jats:sup> is recruited to membranes already at the stage of late endosomes still lacking vacuolar morphology, but the transition between early and late endosomes is sharp, as only in a minor proportion of examples are RabA/RabB and RabS<jats:sup>Rab7</jats:sup> detectable in the same—frequently the less motile—structures. This early-to-late endosome/vacuole transition is coupled to dynein-dependent movement away from the tip, resembling the periphery-to-center traffic of endosomes accompanying mammalian cell endosomal maturation. Genetic studies establish that endosomal maturation is essential, whereas homotypic vacuolar fusion is not. </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381981469073900544","@type":"Researcher","foaf:name":[{"@value":"Juan F. Abenza"}],"jpcoar:affiliationName":[{"@value":"Departamento de Medicina Molecular y Celular, Centro de Investigaciones Biológicas del Consejo Superior de Investigaciones Cientificas, 28040 Madrid, Spain"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469073900545","@type":"Researcher","foaf:name":[{"@value":"Antonio Galindo"}],"jpcoar:affiliationName":[{"@value":"Departamento de Medicina Molecular y Celular, Centro de Investigaciones Biológicas del Consejo Superior de Investigaciones Cientificas, 28040 Madrid, Spain"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469073900417","@type":"Researcher","foaf:name":[{"@value":"Mario Pinar"}],"jpcoar:affiliationName":[{"@value":"Departamento de Medicina Molecular y Celular, Centro de Investigaciones Biológicas del Consejo Superior de Investigaciones Cientificas, 28040 Madrid, Spain"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469073900420","@type":"Researcher","foaf:name":[{"@value":"Areti Pantazopoulou"}],"jpcoar:affiliationName":[{"@value":"Departamento de Medicina Molecular y Celular, Centro de Investigaciones Biológicas del Consejo Superior de Investigaciones Cientificas, 28040 Madrid, Spain"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469073900419","@type":"Researcher","foaf:name":[{"@value":"Vivian de los Ríos"}],"jpcoar:affiliationName":[{"@value":"Departamento de Medicina Molecular y Celular, Centro de Investigaciones Biológicas del Consejo Superior de Investigaciones Cientificas, 28040 Madrid, Spain"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469073900416","@type":"Researcher","foaf:name":[{"@value":"Miguel A. Peñalva"}],"jpcoar:affiliationName":[{"@value":"Departamento de Medicina Molecular y Celular, Centro de Investigaciones Biológicas del Consejo Superior de Investigaciones Cientificas, 28040 Madrid, Spain"}]}],"contributor":[{"@id":"https://cir.nii.ac.jp/crid/1381981469073900418","@type":"Researcher","foaf:name":[{"@value":"Patrick J. Brennwald"}],"jpcoar:affiliationName":[{"@value":"University of North Carolina"}],"role":"editor"}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"10591524"},{"@type":"EISSN","@value":"19394586"}],"prism:publicationName":[{"@value":"Molecular Biology of the Cell"}],"dc:publisher":[{"@value":"American Society for Cell Biology (ASCB)"}],"prism:publicationDate":"2012-05-15","prism:volume":"23","prism:number":"10","prism:startingPage":"1889","prism:endingPage":"1901"},"reviewed":"false","createdAt":"2012-03-29","modifiedAt":"2019-07-14","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360294643760960640","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Membrane traffic related to endosome dynamics and protein secretion in filamentous fungi"}]},{"@id":"https://cir.nii.ac.jp/crid/1360306904390743424","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Subcellular compartmentalized localization of transmembrane proteins essential for production of fungal cyclic peptide cyclochlorotine"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182323934592","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Vesicle trafficking, organelle functions, and unconventional secretion in fungal physiology and pathogenicity"}]},{"@id":"https://cir.nii.ac.jp/crid/1360857593714833664","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Membrane Traffic in Aspergillus oryzae and Related Filamentous Fungi"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1091/mbc.e11-11-0925"},{"@type":"CROSSREF","@value":"10.1093/bbb/zbab004_references_DOI_M7mPfwBKG64YBuWBYzE8i3aPRye"},{"@type":"CROSSREF","@value":"10.1016/j.mib.2014.03.002_references_DOI_M7mPfwBKG64YBuWBYzE8i3aPRye"},{"@type":"CROSSREF","@value":"10.1093/bbb/zbae122_references_DOI_M7mPfwBKG64YBuWBYzE8i3aPRye"},{"@type":"CROSSREF","@value":"10.3390/jof7070534_references_DOI_M7mPfwBKG64YBuWBYzE8i3aPRye"}]}