PEROXIDASE-LABELED ANTIBODY A NEW METHOD OF CONJUGATION

DOI Web Site 被引用文献26件
  • PAUL K. NAKANE
    Department of Pathology, University of Colorado Medical Center, Denver, Colorado 80220
  • AKIRA KAWAOI
    Department of Pathology, University of Colorado Medical Center, Denver, Colorado 80220

説明

<jats:p> A new method of conjugating horseradish peroxidase with proteins was developed. The carbohydrate moiety of fluorodinitrobenzene-blocked peroxidase was oxidized with sodium periodate to form aldehyde groups. The peroxidase-aldehyde was then bound to free amino groups of proteins unidirectionally at high efficiencies. Peroxidase-labeled immunoglobulin retained its immunologic as well as enzymatic activities. </jats:p>

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