{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361981469916448384.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1091/mbc.e07-09-0973"}},{"identifier":{"@type":"PMID","@value":"18287530"}}],"dc:title":[{"@value":"Tight Junction Proteins Claudin-2 and -12 Are Critical for Vitamin D-dependent Ca\n                    <sup>2+</sup>\n                    Absorption between Enterocytes"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>\n                    Ca\n                    <jats:sup>2+</jats:sup>\n                    is absorbed across intestinal epithelial monolayers via transcellular and paracellular pathways, and an active form of vitamin D\n                    <jats:sub>3</jats:sub>\n                    , 1α,25-dihydroxyvitamin D\n                    <jats:sub>3</jats:sub>\n                    [1α,25(OH)\n                    <jats:sub>2</jats:sub>\n                    D\n                    <jats:sub>3</jats:sub>\n                    ], is known to promote intestinal Ca\n                    <jats:sup>2+</jats:sup>\n                    absorption. However, the molecules driving the paracellular Ca\n                    <jats:sup>2+</jats:sup>\n                    absorption and its vitamin D dependency remain obscure. Because the tight junction proteins claudins are suggested to form paracellular channels for selective ions between neighboring cells, we hypothesized that specific intestinal claudins might facilitate paracellular Ca\n                    <jats:sup>2+</jats:sup>\n                    transport and that expression of these claudins could be induced by 1α,25(OH)\n                    <jats:sub>2</jats:sub>\n                    D\n                    <jats:sub>3</jats:sub>\n                    . Herein, we show, by using RNA interference and overexpression strategies, that claudin-2 and claudin-12 contribute to Ca\n                    <jats:sup>2+</jats:sup>\n                    absorption in intestinal epithelial cells. We also provide evidence showing that expression of claudins-2 and -12 is up-regulated in enterocytes in vitro and in vivo by 1α,25(OH)\n                    <jats:sub>2</jats:sub>\n                    D\n                    <jats:sub>3</jats:sub>\n                    through the vitamin D receptor. These findings strongly suggest that claudin-2- and/or claudin-12-based tight junctions form paracellular Ca\n                    <jats:sup>2+</jats:sup>\n                    channels in intestinal epithelia, and they highlight a novel mechanism behind vitamin D-dependent calcium homeostasis.\n                  </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381981469916448391","@type":"Researcher","foaf:name":[{"@value":"Hiroki Fujita"}],"jpcoar:affiliationName":[{"@value":"*Departments of Pathology and"},{"@value":"Orthopedic Surgery, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan;"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469916448384","@type":"Researcher","foaf:name":[{"@value":"Kotaro Sugimoto"}],"jpcoar:affiliationName":[{"@value":"*Departments of Pathology and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469916448387","@type":"Researcher","foaf:name":[{"@value":"Shuichiro Inatomi"}],"jpcoar:affiliationName":[{"@value":"*Departments of Pathology and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469916448261","@type":"Researcher","foaf:name":[{"@value":"Toshihiro Maeda"}],"jpcoar:affiliationName":[{"@value":"*Departments of Pathology and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469916448256","@type":"Researcher","foaf:name":[{"@value":"Makoto Osanai"}],"jpcoar:affiliationName":[{"@value":"*Departments of Pathology and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469916448257","@type":"Researcher","foaf:name":[{"@value":"Yasushi Uchiyama"}],"jpcoar:affiliationName":[{"@value":"Chugai Pharmaceutical Co., Ltd., Tokyo 104-8301, Japan;"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469916448258","@type":"Researcher","foaf:name":[{"@value":"Yoko Yamamoto"}],"jpcoar:affiliationName":[{"@value":"Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113-0032, Japan; and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469916448392","@type":"Researcher","foaf:name":[{"@value":"Takuro Wada"}],"jpcoar:affiliationName":[{"@value":"Orthopedic Surgery, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan;"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469916448259","@type":"Researcher","foaf:name":[{"@value":"Takashi Kojima"}],"jpcoar:affiliationName":[{"@value":"*Departments of Pathology and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469916448386","@type":"Researcher","foaf:name":[{"@value":"Hiroshi Yokozaki"}],"jpcoar:affiliationName":[{"@value":"Division of Surgical Pathology, Department of Biomedical Informatics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469916448390","@type":"Researcher","foaf:name":[{"@value":"Toshihiko Yamashita"}],"jpcoar:affiliationName":[{"@value":"Orthopedic Surgery, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan;"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469916448388","@type":"Researcher","foaf:name":[{"@value":"Shigeaki Kato"}],"jpcoar:affiliationName":[{"@value":"Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113-0032, Japan; and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469916448389","@type":"Researcher","foaf:name":[{"@value":"Norimasa Sawada"}],"jpcoar:affiliationName":[{"@value":"*Departments of Pathology and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469916448260","@type":"Researcher","foaf:name":[{"@value":"Hideki Chiba"}],"jpcoar:affiliationName":[{"@value":"*Departments of Pathology and"}]}],"contributor":[{"@id":"https://cir.nii.ac.jp/crid/1381981469916448385","@type":"Researcher","foaf:name":[{"@value":"Asma Nusrat"}],"role":"editor"}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"10591524"},{"@type":"EISSN","@value":"19394586"}],"prism:publicationName":[{"@value":"Molecular Biology of the Cell"}],"dc:publisher":[{"@value":"American Society for Cell Biology (ASCB)"}],"prism:publicationDate":"2008-05","prism:volume":"19","prism:number":"5","prism:startingPage":"1912","prism:endingPage":"1921"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","createdAt":"2008-02-20","modifiedAt":"2019-07-14","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Mice,%20Knockout","dc:title":"Mice, Knockout"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Membrane%20Permeability","dc:title":"Cell Membrane Permeability"},{"@id":"https://cir.nii.ac.jp/all?q=Sodium","dc:title":"Sodium"},{"@id":"https://cir.nii.ac.jp/all?q=Down-Regulation","dc:title":"Down-Regulation"},{"@id":"https://cir.nii.ac.jp/all?q=Membrane%20Proteins","dc:title":"Membrane Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Biological%20Transport","dc:title":"Biological Transport"},{"@id":"https://cir.nii.ac.jp/all?q=Tight%20Junctions","dc:title":"Tight Junctions"},{"@id":"https://cir.nii.ac.jp/all?q=Intestines","dc:title":"Intestines"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Enterocytes","dc:title":"Enterocytes"},{"@id":"https://cir.nii.ac.jp/all?q=Intestinal%20Absorption","dc:title":"Intestinal Absorption"},{"@id":"https://cir.nii.ac.jp/all?q=Claudins","dc:title":"Claudins"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Receptors,%20Calcitriol","dc:title":"Receptors, Calcitriol"},{"@id":"https://cir.nii.ac.jp/all?q=Calcium","dc:title":"Calcium"},{"@id":"https://cir.nii.ac.jp/all?q=Caco-2%20Cells","dc:title":"Caco-2 Cells"},{"@id":"https://cir.nii.ac.jp/all?q=Intestinal%20Mucosa","dc:title":"Intestinal Mucosa"},{"@id":"https://cir.nii.ac.jp/all?q=Vitamin%20D","dc:title":"Vitamin 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