{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361981469935155456.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1093/pcp/pcj012"}},{"identifier":{"@type":"URI","@value":"http://academic.oup.com/pcp/article-pdf/47/4/457/19670955/pcj012.pdf"}},{"identifier":{"@type":"PMID","@value":"16446312"}}],"dc:title":[{"@value":"Isolation of cDNAs for R2R3-MYB, bHLH and WDR Transcriptional Regulators and Identification of c and ca Mutations Conferring White Flowers in the Japanese Morning Glory"}],"description":[{"notation":[{"@value":"The transcriptional regulators for anthocyanin biosynthesis include members of proteins containing an R2R3-MYB domain, a bHLH (basic helix-loop-helix) domain and conserved WD40 repeats (WDRs). Spacial and temporal expression of the structural genes encoding the enzymes for anthocyanin biosynthesis is thought to be determined by combinations of the R2R3-MYB, bHLH and WDR factors and their interactions. While the wild-type Japanese morning glory (Ipomoea nil) exhibits blue flowers with colored stems and dark-brown seeds, the c mutants display white flowers with red stems and colored seeds, and the ca mutants exhibit white flowers with green stems and ivory seeds. Here, we characterize the tissue-specific expression of three MYB genes, three bHLH genes and two WDR genes in I. nil. We also show that the recessive c-1 and ca alleles are frameshift mutations caused by a 2 bp deletion and 7 bp insertions in the genes for the R2R3-MYB and WDR transcriptional regulators designated as InMYB1 and InWDR1, respectively. In addition to defects in flower, stem and seed pigmentations, the ca mutants were found to show reduced trichome formation in seeds but to produce leaf and stem trichomes and root hairs normally. Except for the gene for chalcone synthase E in the ca mutant, all structural genes tested were coordinately reduced in both c-1 and ca mutant flower limbs. However, slight but significant expression of the genes for chalcone synthase D, chalcone isomerase and flavanone 3-hydroxylase in the pathway for flavonol biosynthesis was detectable in c-1 and ca mutants, whereas no such residual expression could be observed in other genes involved in the later anthocyanin biosynthesis pathway. The biological roles of the C-1 and Ca genes in I. nil epidermal traits and their evolutionary implications are also discussed."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381981469935155456","@type":"Researcher","foaf:name":[{"@value":"Yasumasa Morita"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469935155458","@type":"Researcher","foaf:name":[{"@value":"Miho Saitoh"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469935155460","@type":"Researcher","foaf:name":[{"@value":"Atsushi Hoshino"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469935155457","@type":"Researcher","foaf:name":[{"@value":"Eiji Nitasaka"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981469935155459","@type":"Researcher","foaf:name":[{"@value":"Shigeru Iida"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"14719053"},{"@type":"PISSN","@value":"00320781"}],"prism:publicationName":[{"@value":"Plant and Cell Physiology"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2006-04-01","prism:volume":"47","prism:number":"4","prism:startingPage":"457","prism:endingPage":"470"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://academic.oup.com/pcp/article-pdf/47/4/457/19670955/pcj012.pdf"}],"createdAt":"2006-01-31","modifiedAt":"2021-07-23","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=DNA,%20Complementary","dc:title":"DNA, Complementary"},{"@id":"https://cir.nii.ac.jp/all?q=DNA,%20Plant","dc:title":"DNA, Plant"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Sequence%20Data","dc:title":"Molecular Sequence Data"},{"@id":"https://cir.nii.ac.jp/all?q=Genes,%20Recessive","dc:title":"Genes, Recessive"},{"@id":"https://cir.nii.ac.jp/all?q=Flowers","dc:title":"Flowers"},{"@id":"https://cir.nii.ac.jp/all?q=Genes,%20Plant","dc:title":"Genes, Plant"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression%20Regulation,%20Enzymologic","dc:title":"Gene Expression Regulation, Enzymologic"},{"@id":"https://cir.nii.ac.jp/all?q=Mixed%20Function%20Oxygenases","dc:title":"Mixed Function Oxygenases"},{"@id":"https://cir.nii.ac.jp/all?q=Anthocyanins","dc:title":"Anthocyanins"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression%20Regulation,%20Plant","dc:title":"Gene Expression Regulation, Plant"},{"@id":"https://cir.nii.ac.jp/all?q=Basic%20Helix-Loop-Helix%20Transcription%20Factors","dc:title":"Basic Helix-Loop-Helix Transcription Factors"},{"@id":"https://cir.nii.ac.jp/all?q=Amino%20Acid%20Sequence","dc:title":"Amino Acid Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Intramolecular%20Lyases","dc:title":"Intramolecular Lyases"},{"@id":"https://cir.nii.ac.jp/all?q=Alleles","dc:title":"Alleles"},{"@id":"https://cir.nii.ac.jp/all?q=Base%20Sequence","dc:title":"Base Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Pigmentation","dc:title":"Pigmentation"},{"@id":"https://cir.nii.ac.jp/all?q=Mutation","dc:title":"Mutation"},{"@id":"https://cir.nii.ac.jp/all?q=Seeds","dc:title":"Seeds"},{"@id":"https://cir.nii.ac.jp/all?q=Ipomoea","dc:title":"Ipomoea"},{"@id":"https://cir.nii.ac.jp/all?q=Acyltransferases","dc:title":"Acyltransferases"},{"@id":"https://cir.nii.ac.jp/all?q=Transcription%20Factors","dc:title":"Transcription Factors"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050001335796866688","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"A bHLH transcription factor, DvIVS, is involved in regulation of anthocyanin synthesis in dahlia (Dahlia variabilis)."}]},{"@id":"https://cir.nii.ac.jp/crid/1360002215451408896","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A review of target gene specificity of flavonoid R2R3-MYB transcription factors and a discussion of factors contributing to the target gene selectivity"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004231206138496","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Characterization of a heavy-ion induced white flower mutant of allotetraploid Nicotiana tabacum"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004235809716992","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A petal‐specific <i>In<scp>MYB</scp>1</i> promoter from Japanese morning glory: a useful tool for molecular breeding of floricultural crops"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004235827103872","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A chalcone isomerase‐like protein enhances flavonoid production and flower pigmentation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360017280638336000","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Genome-wide analysis of R2R3-MYB transcription factors in Japanese morning glory"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283690390769536","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Involvement of a LhMYB18 transcription factor in large anthocyanin spot formation on the flower tepals of the Asiatic hybrid lily (Lilium spp.) cultivar “Grand Cru”"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285706215582464","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Spontaneous mutations of the UDP-glucose:flavonoid 3-O-glucosyltransferase gene confers pale- and dull-colored flowers in the Japanese and common morning glories"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285709716845312","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The Petal-Specific<i>InMYB1</i>Promoter Functions by Recognizing Petaloid Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302866835566464","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Petal abscission is promoted by jasmonic acid-induced autophagy at Arabidopsis petal bases"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565165367320832","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Oriental hybrid lily Sorbonne homologue of LhMYB12 regulates anthocyanin biosyntheses in flower tepals and tepal spots"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846640344137984","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Two distinct spontaneous mutations involved in white flower development in Lilium speciosum"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848656283639936","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Anthocyanin and carotenoid pigmentation in flowers of section Mina, subgenus Quamoclit, genus Ipomoea"}]},{"@id":"https://cir.nii.ac.jp/crid/1360869855126125696","@type":"Article","resourceType":"preprint","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Transcriptomic dynamics of petal development in the one-day flower species, Japanese morning glory (\n                  <i>Ipomoea nil</i>\n                  )"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204326607488","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Transcriptional regulators of flavonoid biosynthesis and their application to flower color modification in Japanese gentians"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204723038976","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Recent advances in flower color variation and patterning of Japanese morning glory and petunia"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205474729472","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"A <i>Stowaway</i> transposon disrupts the <i>InWDR1</i> gene controlling flower and seed coloration in a medicinal cultivar of the Japanese morning glory"},{"@value":"A Stowaway transposon disrupts the InWDR1 gene controlling flower and seed coloration in a medicinal cultivar of the Japanese morning glory"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205760191360","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"トルコギキョウ［<i>Eustoma grandiflorum</i> (Raf.) Shinn.］由来のフラボノイド 3′- 水酸化酵素遺伝子（<i>EgF3′H</i>）の単離及び <i>F3</i>′<i>H</i> 機能欠失変異アサガオ［<i>Ipomoea nil</i> (L.) Roth.］を用いた <i>EgF3</i>′<i>H</i> の異種発現による相補性検定"},{"@language":"en","@value":"Cloning of the Flavonoid 3'-Hydroxylase Gene of <i>Eustoma grandiflorum</i> (Raf.) Shinn. (<i>EgF3'H</i>) and Complementation of an F3'H-deficient Mutant of <i>Ipomoea nil</i> (L.) Roth. by Heterologous Expression of <i>EgF3'H</i>"},{"@value":"Cloning of the Flavonoid 3'-Hydroxylase Gene of Eustoma grandiflorum (Raf.) Shinn. (EgF3'H) and Complementation of an F3'H-deficient Mutant of Ipomoea nil (L.) Roth. by Heterologous Expression of EgF3'H"},{"@value":"Cloning of the Flavonoid 3&apos;-Hydroxylase Gene of <i>Eustoma grandiflorum</i> (Raf.) Shinn. (<i>EgF3&apos;H</i>) and Complementation of an F3&apos;H-deficient Mutant of <i>Ipomoea nil</i> (L.) Roth. by Heterologous Expression of <i>EgF3&apos;H</i>"},{"@value":"Cloning of the flavonoid 3′-hydroxylase gene of Eustoma grandiflorum (Raf.) Shinn. (EgF3′H) and complementation of an F3′H-deficient mutant of Ipomoea nil (L.) Roth. by heterologous expression of EgF3′"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001288079983744","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Dissecting promoter of <i>InMYB1</i> gene showing petal-specific expression"},{"@value":"Dissecting promoter of InMYB1 gene showing petal-specific expression"}]},{"@id":"https://cir.nii.ac.jp/crid/1390290846630648064","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"A Torenia Mutant Bearing Shrunken Reddish-purple Flower and its Potential for Breeding"}]},{"@id":"https://cir.nii.ac.jp/crid/1390291932641860352","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Flavonoid 3',5'-Hydroxylase (<i>F3'5'H</i>) Gene Polymorphisms Co-segregate with Variation in Anthocyanin Composition in the Flower Petals of Lisianthus [<i>Eustoma grandiflorum</i> (Raf.) Shinn.]"},{"@value":"Flavonoid 3',5'-Hydroxylase (F3'5'H) Gene Polymorphisms Co-segregate with Variation in Anthocyanin Composition in the Flower Petals of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]"}]},{"@id":"https://cir.nii.ac.jp/crid/1390572321603107840","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Localized Repression of Two bHLH Genes is Involved in the Formation of White Margins and White Abaxial Surfaces in Carnation Petals by Inducing the Absence of Anthocyanin Synthesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1390845713006388992","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Anthocyanin mutants of Japanese and common morning glories exhibit normal proanthocyanidin accumulation in seed coats"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1093/pcp/pcj012"},{"@type":"OPENAIRE","@value":"doi_dedup___::778a5558059d298444d9274a7d9247c7"},{"@type":"CROSSREF","@value":"10.1007/s11515-013-1281-z_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.1007/s00299-012-1336-7_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.1111/pbi.12389_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.1111/tpj.12469_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.14.0731a_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.18.0529a_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.2503/hortj.utd-320_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.1371/journal.pone.0271012_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.1007/s11032-018-0806-1_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.1007/s00425-015-2321-5_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.1093/pcp/pcw017_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.1270/jsbbs.17107_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.18.0613a_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.2503/hortj.utd-313_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.2503/hortj.utd-319_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.1038/s41467-024-45371-3_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.1007/s11032-010-9490-5_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.1093/jxb/err216_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.2503/hortj.mi-029_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.1266/ggs.15-00062_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.1007/s11032-015-0389-z_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.1007/s10681-011-0618-4_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"},{"@type":"CROSSREF","@value":"10.1101/2024.08.29.610218_references_DOI_UNstbZwAyFgdISyBf33s83P0aBG"}]}