{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361981470194428800.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1063/1.3122007"}},{"identifier":{"@type":"URI","@value":"https://pubs.aip.org/aip/jap/article-pdf/doi/10.1063/1.3122007/15050651/094905_1_online.pdf"}}],"dc:title":[{"@value":"Image formation, resolution, and height measurement in scanning ion conductance microscopy"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Scanning ion conductance microscopy (SICM) is an emerging tool for the noncontact investigation of biological samples such as live cells. It uses an ion current through the opening of a tapered nanopipette filled with an electrolyte for topography measurements. Despite its successful application to numerous systems no systematic investigation of the image formation process has yet been performed. Here, we use finite element modeling to investigate how the scanning ion conductance microscope images small particles on a planar surface, providing a fundamental characterization of the imaging process. We find that a small particle appears with a height that is only a fraction of its actual height. This has significant consequences for the quantitative interpretation of SICM images. Furthermore, small and low particles are imaged as rings in certain cases. This can cause small, closely spaced particles to appear with a lateral orientation that is rotated by 90°. Considering both real space and spatial frequency space we find that a reasonable and useful definition of lateral resolution of SICM is the smallest distance at which two small particles can clearly be resolved from each other in an image. We find that this resolution is approximately equal to three times the inner radius of the pipette tip opening.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381981470194428801","@type":"Researcher","foaf:name":[{"@value":"Johannes Rheinlaender"}],"jpcoar:affiliationName":[{"@value":"University of Erlangen-Nuremberg Institute of Applied Physics, , Staudtstr. 7, Bldg. A3, 91058 Erlangen, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981470194428800","@type":"Researcher","foaf:name":[{"@value":"Tilman E. Schäffer"}],"jpcoar:affiliationName":[{"@value":"University of Erlangen-Nuremberg Institute of Applied Physics, , Staudtstr. 7, Bldg. A3, 91058 Erlangen, Germany"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00218979"},{"@type":"EISSN","@value":"10897550"}],"prism:publicationName":[{"@value":"Journal of Applied Physics"}],"dc:publisher":[{"@value":"AIP Publishing"}],"prism:publicationDate":"2009-05-01","prism:volume":"105","prism:number":"9","prism:startingPage":"094905"},"reviewed":"false","url":[{"@id":"https://pubs.aip.org/aip/jap/article-pdf/doi/10.1063/1.3122007/15050651/094905_1_online.pdf"}],"createdAt":"2009-05-07","modifiedAt":"2023-06-25","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004232372513280","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Scanning ion conductance microscopy for imaging biological samples in liquid: A comparative study with atomic force microscopy and scanning electron microscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360009142602641920","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Mapping mechanical properties of living cells at nanoscale using intrinsic nanopipette–sample force interactions"}]},{"@id":"https://cir.nii.ac.jp/crid/1360009142872537856","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Geometrical Characterization of Glass Nanopipettes with Sub-10 nm Pore Diameter by Transmission Electron Microscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285707300146944","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Time-lapse imaging of morphological changes in a single neuron during the early stages of apoptosis using scanning ion conductance microscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360847874820263296","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Recent advances in micromechanical characterization of polymer, biomaterial, and cell surfaces with atomic force microscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865815509005824","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Nanopipette Fabrication Guidelines for SICM Nanoscale Imaging"}]},{"@id":"https://cir.nii.ac.jp/crid/1361975843659973632","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Development of high-speed ion conductance microscopy"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1063/1.3122007"},{"@type":"CROSSREF","@value":"10.1039/d0nr08349f_references_DOI_Ef7mctQcYPO1hq2WzLcpAOsiGND"},{"@type":"CROSSREF","@value":"10.1021/acs.analchem.0c02884_references_DOI_Ef7mctQcYPO1hq2WzLcpAOsiGND"},{"@type":"CROSSREF","@value":"10.7567/jjap.54.08la02_references_DOI_Ef7mctQcYPO1hq2WzLcpAOsiGND"},{"@type":"CROSSREF","@value":"10.1021/acs.analchem.3c01010_references_DOI_Ef7mctQcYPO1hq2WzLcpAOsiGND"},{"@type":"CROSSREF","@value":"10.1016/j.micron.2012.01.012_references_DOI_Ef7mctQcYPO1hq2WzLcpAOsiGND"},{"@type":"CROSSREF","@value":"10.1016/j.jsb.2015.06.002_references_DOI_Ef7mctQcYPO1hq2WzLcpAOsiGND"},{"@type":"CROSSREF","@value":"10.1063/1.5118360_references_DOI_Ef7mctQcYPO1hq2WzLcpAOsiGND"}]}