{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361981470303532032.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/ec.4.11.1765-1774.2005"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/EC.4.11.1765-1774.2005"}}],"dc:title":[{"@value":"Accelerated Cell Death in\n            <i>Podospora</i>\n            Autophagy Mutants"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Although autophagy is characteristic of type II programmed cell death (PCD), its role in cell death is currently debated. Both cell death-promoting and prosurvival roles of autophagy have been reported depending on the organism and the cell type. In filamentous fungi, a cell death reaction known as an incompatibility reaction occurs when cells of unlike genotype fuse. Cell death by incompatibility is characterized by a dramatic vacuolar enlargement and cell lysis. In\n            <jats:italic>Podospora anserina</jats:italic>\n            , autophagy is induced early during this cell death reaction. Cell death by incompatibility in\n            <jats:italic>Podospora</jats:italic>\n            is a model of type II PCD used here to assess the role of autophagy in this type of cell death. We have inactivated Pa\n            <jats:italic>ATG1</jats:italic>\n            , the\n            <jats:italic>Podospora</jats:italic>\n            ortholog of the\n            <jats:italic>Saccharomyces cerevisiae ATG1</jats:italic>\n            gene involved in the early steps of autophagy in yeast. The ΔPa\n            <jats:italic>ATG1</jats:italic>\n            mutant displays developmental defects characteristic of abrogated autophagy in\n            <jats:italic>Podospora</jats:italic>\n            . Using the green fluorescent protein-PaATG8 autophagosome marker, we show that autophagy is abolished in this mutant. Neither cell death by incompatibility nor vacuolization are suppressed in ΔPa\n            <jats:italic>ATG1</jats:italic>\n            and ΔPa\n            <jats:italic>ATG8</jats:italic>\n            autophagy mutants, indicating that a vacuolar cell death reaction without autophagy occurs in\n            <jats:italic>Podospora</jats:italic>\n            . Our results thus provide a novel example of a type II PCD reaction in which autophagy is not the cause of cell death. In addition, we found that cell death is accelerated in ΔPa\n            <jats:italic>ATG</jats:italic>\n            null mutants, suggesting that autophagy has a protective role in this type II PCD reaction.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381981470303532033","@type":"Researcher","foaf:name":[{"@value":"Bérangère Pinan-Lucarré"}],"jpcoar:affiliationName":[{"@value":"Laboratoire de Génétique Moléculaire des Champignons, Institut de Biochimie et de Génétique Cellulaires, UMR 5095 CNRS et Université de Bordeaux 2, Bordeaux, France"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981470303532034","@type":"Researcher","foaf:name":[{"@value":"Axelle Balguerie"}],"jpcoar:affiliationName":[{"@value":"Laboratoire de Génétique Moléculaire des Champignons, Institut de Biochimie et de Génétique Cellulaires, UMR 5095 CNRS et Université de Bordeaux 2, Bordeaux, France"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981470303532032","@type":"Researcher","foaf:name":[{"@value":"Corinne Clavé"}],"jpcoar:affiliationName":[{"@value":"Laboratoire de Génétique Moléculaire des Champignons, Institut de Biochimie et de Génétique Cellulaires, UMR 5095 CNRS et Université de Bordeaux 2, Bordeaux, France"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"15359778"},{"@type":"EISSN","@value":"15359786"}],"prism:publicationName":[{"@value":"Eukaryotic Cell"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"2005-11","prism:volume":"4","prism:number":"11","prism:startingPage":"1765","prism:endingPage":"1774"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/EC.4.11.1765-1774.2005"}],"createdAt":"2005-11-08","modifiedAt":"2022-02-23","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360283692333057792","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)"}]},{"@id":"https://cir.nii.ac.jp/crid/1360290617882504960","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)<sup>1</sup>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182323934592","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Vesicle trafficking, organelle functions, and unconventional secretion in fungal physiology and pathogenicity"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/ec.4.11.1765-1774.2005"},{"@type":"CROSSREF","@value":"10.1080/15548627.2015.1100356_references_DOI_XrCliVBQzrbRhl2M2KVK8k12Met"},{"@type":"CROSSREF","@value":"10.4161/auto.19496_references_DOI_XrCliVBQzrbRhl2M2KVK8k12Met"},{"@type":"CROSSREF","@value":"10.1080/15548627.2020.1797280_references_DOI_XrCliVBQzrbRhl2M2KVK8k12Met"},{"@type":"CROSSREF","@value":"10.1016/j.mib.2014.03.002_references_DOI_XrCliVBQzrbRhl2M2KVK8k12Met"}]}