{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361981470416958720.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1093/genetics/156.3.963"}},{"identifier":{"@type":"URI","@value":"https://academic.oup.com/genetics/article-pdf/156/3/963/42032122/genetics0963.pdf"}}],"dc:title":[{"@value":"A Genetic Analysis of Glucocorticoid Receptor Signaling: Identification and Characterization of Ligand-Effect Modulators in <i>Saccharomyces cerevisiae</i>"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n               <jats:p>To find novel components in the glucocorticoid signal transduction pathway, we performed a yeast genetic screen to identify ligand-effect modulators (LEMs), proteins that modulate the cellular response to hormone. We isolated several mutants that conferred increased glucocorticoid receptor (GR) activity in response to dexamethasone and analyzed two of them in detail. These studies identify two genes, LEM3 and LEM4, which correspond to YNL323w and ERG6, respectively. LEM3 is a putative transmembrane protein of unknown function, and ERG6 is a methyltransferase in the ergosterol biosynthetic pathway. Analysis of null mutants indicates that LEM3 and ERG6 act at different steps in the GR signal transduction pathway.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381981470416958722","@type":"Researcher","foaf:name":[{"@value":"Raquel Sitcheran"}],"jpcoar:affiliationName":[{"@value":"Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 91413-0450"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981470416958720","@type":"Researcher","foaf:name":[{"@value":"Roger Emter"}],"jpcoar:affiliationName":[{"@value":"Division of Biochemistry, Biozentrum, University of Basel, Basel CH4056, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981470416958592","@type":"Researcher","foaf:name":[{"@value":"Anastasia Kralli"}],"jpcoar:affiliationName":[{"@value":"Division of Biochemistry, Biozentrum, University of Basel, Basel CH4056, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981470416958721","@type":"Researcher","foaf:name":[{"@value":"Keith R Yamamoto"}],"jpcoar:affiliationName":[{"@value":"Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 91413-0450"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"19432631"}],"prism:publicationName":[{"@value":"Genetics"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2000-11-01","prism:volume":"156","prism:number":"3","prism:startingPage":"963","prism:endingPage":"972"},"reviewed":"false","dc:rights":["https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model"],"url":[{"@id":"https://academic.oup.com/genetics/article-pdf/156/3/963/42032122/genetics0963.pdf"}],"createdAt":"2021-04-28","modifiedAt":"2022-01-04","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050001335849297024","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"New reporter gene assays for detecting natural and synthetic molting hormone agonists using yeasts expressing ecdysone receptors of various insects"}]},{"@id":"https://cir.nii.ac.jp/crid/1050023239833531776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Development of a New Reporter Gene Assay for Detecting Juvenile Hormone Agonists Using Yeast Expressing Methoprene-Tolerant of the Freshwater Cladoceran Daphnia magna"},{"@value":"Development of a New Reporter Gene Assay for Detecting Juvenile Hormone Agonists Using Yeast Expressing Methoprene‐Tolerant of the Freshwater Cladoceran <scp><i>Daphnia magna</i></scp>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360294643778257408","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Detection of juvenile hormone agonists by a new reporter gene assay using yeast expressing <i>Drosophila</i> methoprene‐tolerant"}]},{"@id":"https://cir.nii.ac.jp/crid/1360584339749251200","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Development of a yeast reporter gene assay to detect ligands of freshwater cladoceran <scp><i>Daphnia magna</i></scp> ultraspiracle, a homolog of vertebrate retinoid X receptors"}]},{"@id":"https://cir.nii.ac.jp/crid/2050588892096568704","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Construction of reporter gene assays using CWP and PDR mutant yeasts for enhanced detection of various sex steroids"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1093/genetics/156.3.963"},{"@type":"CROSSREF","@value":"10.1002/2211-5463.12239_references_DOI_Z7Zd475kngdMss3Cqf94YtjIn5n"},{"@type":"CROSSREF","@value":"10.1002/jat.4784_references_DOI_Z7Zd475kngdMss3Cqf94YtjIn5n"},{"@type":"CROSSREF","@value":"10.1002/2211-5463.13277_references_DOI_Z7Zd475kngdMss3Cqf94YtjIn5n"},{"@type":"CROSSREF","@value":"10.1002/jat.4476_references_DOI_Z7Zd475kngdMss3Cqf94YtjIn5n"},{"@type":"CROSSREF","@value":"10.1186/s41021-020-00159-x_references_DOI_Z7Zd475kngdMss3Cqf94YtjIn5n"}]}