{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361981471036704640.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1104/pp.125.2.539"}},{"identifier":{"@type":"URI","@value":"http://academic.oup.com/plphys/article-pdf/125/2/539/38678644/plphys_v125_2_539.pdf"}}],"dc:title":[{"@value":"An Investigation of the Storage and Biosynthesis of Phenylpropenes in Sweet Basil"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n               <jats:p>Plants that contain high concentrations of the defense compounds of the phenylpropene class (eugenol, chavicol, and their derivatives) have been recognized since antiquity as important spices for human consumption (e.g. cloves) and have high economic value. Our understanding of the biosynthetic pathway that produces these compounds in the plant, however, has remained incomplete. Several lines of basil (Ocimum basilicum) produce volatile oils that contain essentially only one or two specific phenylpropene compounds. Like other members of the Lamiaceae, basil leaves possess on their surface two types of glandular trichomes, termed peltate and capitate glands. We demonstrate here that the volatile oil constituents eugenol and methylchavicol accumulate, respectively, in the peltate glands of basil lines SW (which produces essentially only eugenol) and EMX-1 (which produces essentially only methylchavicol). Assays for putative enzymes in the biosynthetic pathway leading to these phenylpropenes localized many of the corresponding enzyme activities almost exclusively to the peltate glands in leaves actively producing volatile oil. An analysis of an expressed sequence tag database from leaf peltate glands revealed that known genes for the phenylpropanoid pathway are expressed at very high levels in these structures, accounting for 13% of the total expressed sequence tags. An additional 14% of cDNAs encoded enzymes for the biosynthesis of S-adenosyl-methionine, an important substrate in the synthesis of many phenylpropenes. Thus, the peltate glands of basil appear to be highly specialized structures for the synthesis and storage of phenylpropenes, and serve as an excellent model system to study phenylpropene biosynthesis.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381981471036704642","@type":"Researcher","foaf:name":[{"@value":"David R. Gang"}],"jpcoar:affiliationName":[{"@value":"Department of Biology, University of Michigan, Ann Arbor, Michigan 48109–1048 (D.R.G., J.W., K.H.N., E. P.);"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981471036704646","@type":"Researcher","foaf:name":[{"@value":"Jihong Wang"}],"jpcoar:affiliationName":[{"@value":"Department of Biology, University of Michigan, Ann Arbor, Michigan 48109–1048 (D.R.G., J.W., K.H.N., E. P.);"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981471036704645","@type":"Researcher","foaf:name":[{"@value":"Natalia Dudareva"}],"jpcoar:affiliationName":[{"@value":"Horticulture Department, Purdue University, West Lafayette, Indiana 47907 (N.D.);"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981471036704641","@type":"Researcher","foaf:name":[{"@value":"Kyoung Hee Nam"}],"jpcoar:affiliationName":[{"@value":"Department of Biology, University of Michigan, Ann Arbor, Michigan 48109–1048 (D.R.G., J.W., K.H.N., E. P.);"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981471036704643","@type":"Researcher","foaf:name":[{"@value":"James E. Simon"}],"jpcoar:affiliationName":[{"@value":"Department of Plant Science, Rutgers University, New Brunswick, New Jersey 08901 (J.E.S.); and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981471036704640","@type":"Researcher","foaf:name":[{"@value":"Efraim Lewinsohn"}],"jpcoar:affiliationName":[{"@value":"Aromatic, Medicinal, and Spice Crops Unit, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel (E.L.)"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981471036704644","@type":"Researcher","foaf:name":[{"@value":"Eran Pichersky"}],"jpcoar:affiliationName":[{"@value":"Department of Biology, University of Michigan, Ann Arbor, Michigan 48109–1048 (D.R.G., J.W., K.H.N., E. P.);"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"15322548"},{"@type":"PISSN","@value":"00320889"}],"prism:publicationName":[{"@value":"Plant Physiology"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2001-02-01","prism:volume":"125","prism:number":"2","prism:startingPage":"539","prism:endingPage":"555"},"reviewed":"false","dc:rights":["https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model"],"url":[{"@id":"http://academic.oup.com/plphys/article-pdf/125/2/539/38678644/plphys_v125_2_539.pdf"}],"createdAt":"2002-07-27","modifiedAt":"2021-06-25","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004235815963136","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Bioactivity of natural <i><scp>O</scp></i>‐prenylated phenylpropenes from <i><scp>I</scp>llicium anisatum</i> leaves and their derivatives against spider mites and fungal pathogens"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004239504920832","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Recent Advances in the Application of Metabolomics to Studies of Biogenic Volatile Organic Compounds (BVOC) Produced  by Plant"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285709149896320","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Functional evolution of biosynthetic enzymes that produce plant volatiles*"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846642581887744","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Expression Profiling of Tobacco Leaf Trichomes Identifies Genes for Biotic and Abiotic Stresses"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848661765300736","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Quantitative assay of targeted proteome in tomato trichome glandular cells using a large-scale selected reaction monitoring strategy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360869855109546368","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Increase of secondary metabolites in sweet basil (Ocimum basilicum L.) leaves by exposure to N2O5 with plasma technology"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204326606208","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Recent progress in secondary metabolism of plant glandular trichomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204327902336","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effect of light quality on rosmarinic acid content and antioxidant activity of sweet basil, Ocimum basilicum L."}]},{"@id":"https://cir.nii.ac.jp/crid/1390009142391287424","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"HPLC Fluorescence Method for Eugenols in Basil Products Derivatized with DIBI"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680196401152","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"ハーブの香気成分の代謝・放出に及ぼす空気中二酸化炭素濃度の影響"},{"@language":"en","@value":"Influence of Carbon Dioxide Concentration in Air on the Metabolite and Release of Flavor Components of Herbs"},{"@language":"ja-Kana","@value":"ハーブ ノ コウキ セイブン ノ タイシャ ホウシュツ ニ オヨボス クウキ チュウ ニサンカ タンソ ノウド ノ エイキョウ"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1104/pp.125.2.539"},{"@type":"CROSSREF","@value":"10.1111/plb.12054_references_DOI_Iur9BFfD3sdok5TLXheBmHYbRok"},{"@type":"CROSSREF","@value":"10.3390/metabo4030699_references_DOI_Iur9BFfD3sdok5TLXheBmHYbRok"},{"@type":"CROSSREF","@value":"10.2171/jao.41.8_references_DOI_Iur9BFfD3sdok5TLXheBmHYbRok"},{"@type":"CROSSREF","@value":"10.1080/09168451.2017.1422968_references_DOI_Iur9BFfD3sdok5TLXheBmHYbRok"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.26.255_references_DOI_Iur9BFfD3sdok5TLXheBmHYbRok"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.14.0701a_references_DOI_Iur9BFfD3sdok5TLXheBmHYbRok"},{"@type":"CROSSREF","@value":"10.1093/pcp/pcq118_references_DOI_Iur9BFfD3sdok5TLXheBmHYbRok"},{"@type":"CROSSREF","@value":"10.1186/s13007-019-0427-7_references_DOI_Iur9BFfD3sdok5TLXheBmHYbRok"},{"@type":"CROSSREF","@value":"10.1248/cpb.c21-00575_references_DOI_Iur9BFfD3sdok5TLXheBmHYbRok"},{"@type":"CROSSREF","@value":"10.1038/s41598-024-63508-8_references_DOI_Iur9BFfD3sdok5TLXheBmHYbRok"}]}