{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361981471344320640.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.carres.2003.10.035"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0008621504000382?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0008621504000382?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"15063189"}}],"dc:title":[{"@value":"Two potent competitive inhibitors discriminating α-glucosidase family I from family II"}],"description":[{"notation":[{"@value":"The inhibition kinetics for isoacarbose (a pseudotetrasaccharide, IsoAca) and acarviosine-glucose (pseudotrisaccharide, AcvGlc), both of which are derivatives of acarbose, were investigated with various types of alpha-glucosidases obtained from microorganisms, plants, and insects. IsoAca and AcvGlc, competitive inhibitors, allowed classification of alpha-glucosidases into two groups. Enzymes of the first group were strongly inhibited by AcvGlc and weakly by IsoAca, in which the K(i) values of AcvGlc (0.35-3.0 microM) were 21- to 440-fold smaller than those of IsoAca. However, the second group of enzymes showed similar K(i) values, ranging from 1.6 to 8.0 microM for both compounds. This classification for alpha-glucosidases is in total agreement with that based on the similarity of their amino acid sequences (family I and family II). This indicated that the alpha-glucosidase families I and II could be clearly distinguished based on their inhibition kinetic data for IsoAca and AcvGlc. The two groups of alpha-glucosidases seemed to recognize distinctively the extra reducing-terminal glucose unit in IsoAca."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381981471344320644","@type":"Researcher","foaf:name":[{"@value":"Atsuo Kimura"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981471344320643","@type":"Researcher","foaf:name":[{"@value":"Jin-Ha Lee"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981471344320640","@type":"Researcher","foaf:name":[{"@value":"In-Su Lee"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981471344320645","@type":"Researcher","foaf:name":[{"@value":"Hee-Seob Lee"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981471344320642","@type":"Researcher","foaf:name":[{"@value":"Kwan-Hwa Park"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981471344320641","@type":"Researcher","foaf:name":[{"@value":"Seiya Chiba"}]},{"@id":"https://cir.nii.ac.jp/crid/1381981471344320646","@type":"Researcher","foaf:name":[{"@value":"Doman Kim"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00086215"}],"prism:publicationName":[{"@value":"Carbohydrate Research"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2004-04","prism:volume":"339","prism:number":"6","prism:startingPage":"1035","prism:endingPage":"1040"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0008621504000382?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0008621504000382?httpAccept=text/plain"}],"createdAt":"2004-02-29","modifiedAt":"2021-06-14","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Oligosaccharides","dc:title":"Oligosaccharides"},{"@id":"https://cir.nii.ac.jp/all?q=Amino%20Sugars","dc:title":"Amino Sugars"},{"@id":"https://cir.nii.ac.jp/all?q=Oryza","dc:title":"Oryza"},{"@id":"https://cir.nii.ac.jp/all?q=alpha-Glucosidases","dc:title":"alpha-Glucosidases"},{"@id":"https://cir.nii.ac.jp/all?q=Saccharomyces%20cerevisiae","dc:title":"Saccharomyces cerevisiae"},{"@id":"https://cir.nii.ac.jp/all?q=Binding,%20Competitive","dc:title":"Binding, Competitive"},{"@id":"https://cir.nii.ac.jp/all?q=Kinetics","dc:title":"Kinetics"},{"@id":"https://cir.nii.ac.jp/all?q=Glucose","dc:title":"Glucose"},{"@id":"https://cir.nii.ac.jp/all?q=Carbohydrate%20Sequence","dc:title":"Carbohydrate Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Models,%20Chemical","dc:title":"Models, Chemical"},{"@id":"https://cir.nii.ac.jp/all?q=Carbohydrate%20Conformation","dc:title":"Carbohydrate Conformation"},{"@id":"https://cir.nii.ac.jp/all?q=Acarbose","dc:title":"Acarbose"},{"@id":"https://cir.nii.ac.jp/all?q=Chromatography,%20Thin%20Layer","dc:title":"Chromatography, Thin Layer"},{"@id":"https://cir.nii.ac.jp/all?q=Enzyme%20Inhibitors","dc:title":"Enzyme Inhibitors"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050285299902639232","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Structure–function analysis of silkworm sucrose hydrolase uncovers the mechanism of substrate specificity in GH13 subfamily 17 exo-α-glucosidases"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848656072432384","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"α-Glucosidases and α-1,4-glucan lyases: structures, functions, and physiological actions"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681456617984","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Chebulagic Acid Is a Potent .ALPHA.-Glucosidase Inhibitor"},{"@value":"Chebulagic acid is a potent α-glucosidase inhibitor"},{"@language":"ja-Kana","@value":"Chebulagic acid is a potent a glucosidase inhibitor"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/j.carres.2003.10.035"},{"@type":"OPENAIRE","@value":"doi_dedup___::88728291be4aecf37d4f507dd5bf011e"},{"@type":"CROSSREF","@value":"10.1074/jbc.ra120.013595_references_DOI_bh10S6g4PMjZTwrnu8KQHR845i"},{"@type":"CROSSREF","@value":"10.1271/bbb.70591_references_DOI_bh10S6g4PMjZTwrnu8KQHR845i"},{"@type":"CROSSREF","@value":"10.1007/s00018-016-2247-5_references_DOI_bh10S6g4PMjZTwrnu8KQHR845i"}]}