{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262943346679424.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1101/gad.1844309"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1101/gad.1844309"}},{"identifier":{"@type":"PMID","@value":"19696148"}}],"dc:title":[{"@value":"Releasing cohesin from chromosome arms in early mitosis: opposing actions of Wapl–Pds5 and Sgo1"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>The cohesin complex establishes sister chromatid cohesion during S phase. In metazoan cells, most if not all cohesin dissociates from chromatin during mitotic prophase, leading to the formation of metaphase chromosomes with two cytologically discernible chromatids. This process, known as sister chromatid resolution, is believed to be a prerequisite for synchronous separation of sister chromatids in subsequent anaphase. To dissect this process at a mechanistic level, we set up an in vitro system. Sister chromatid resolution is severely impaired upon depletion of Wapl from <jats:italic>Xenopus</jats:italic> egg extracts. Exogenously added human Wapl can rescue these defects and, remarkably, it can do so in a very short time window of early mitosis. A similar set of observations is made for Pds5, a factor implicated previously in the stabilization of interphase cohesion. Characteristic amino acid motifs (the FGF motifs) in Wapl coordinate its physical and functional interactions with Pds5 and cohesin subunits. We propose that Wapl and Pds5 directly modulate conformational changes of cohesin to make it competent for dissociation from chromatin during prophase. Evidence is also presented that Sgo1 plays a hitherto underappreciated role in stabilizing cohesin along chromosome arms, which is antagonized by the mitotic kinases polo-like kinsase (Plk1) and aurora B.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262943346679425","@type":"Researcher","foaf:name":[{"@value":"Keishi Shintomi"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943346679424","@type":"Researcher","foaf:name":[{"@value":"Tatsuya Hirano"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"08909369"},{"@type":"EISSN","@value":"15495477"}],"prism:publicationName":[{"@value":"Genes & Development"}],"dc:publisher":[{"@value":"Cold Spring Harbor Laboratory"}],"prism:publicationDate":"2009-08-20","prism:volume":"23","prism:number":"18","prism:startingPage":"2224","prism:endingPage":"2236"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"https://syndication.highwire.org/content/doi/10.1101/gad.1844309"}],"createdAt":"2009-08-21","modifiedAt":"2021-11-16","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Chromosomal%20Proteins,%20Non-Histone","dc:title":"Chromosomal Proteins, Non-Histone"},{"@id":"https://cir.nii.ac.jp/all?q=Xenopus","dc:title":"Xenopus"},{"@id":"https://cir.nii.ac.jp/all?q=Mitosis","dc:title":"Mitosis"},{"@id":"https://cir.nii.ac.jp/all?q=Nuclear%20Proteins","dc:title":"Nuclear Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Cycle%20Proteins","dc:title":"Cell Cycle Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Xenopus%20Proteins","dc:title":"Xenopus Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Phosphoproteins","dc:title":"Phosphoproteins"},{"@id":"https://cir.nii.ac.jp/all?q=Chromosomes","dc:title":"Chromosomes"},{"@id":"https://cir.nii.ac.jp/all?q=Recombinant%20Proteins","dc:title":"Recombinant Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=DNA-Binding%20Proteins","dc:title":"DNA-Binding Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Proto-Oncogene%20Proteins","dc:title":"Proto-Oncogene Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Apoptosis%20Regulatory%20Proteins","dc:title":"Apoptosis Regulatory Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Carrier%20Proteins","dc:title":"Carrier Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Cohesins","dc:title":"Cohesins"},{"@id":"https://cir.nii.ac.jp/all?q=Transcription%20Factors","dc:title":"Transcription Factors"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050575520345884672","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Is age-related increase of chromosome segregation errors in mammalian oocytes caused by cohesin deterioration?"},{"@value":"Is age‐related increase of chromosome segregation errors in mammalian oocytes caused by cohesin deterioration?"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002216789704704","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Xenopus Shugoshin 2 regulates the spindle assembly pathway mediated by the chromosomal passenger complex"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002218438191744","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Two Histone Marks Establish the Inner Centromere and 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