{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262943461494528.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/0471140864.ps1109s58"}},{"identifier":{"@type":"URI","@value":"http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2F0471140864.ps1109s58"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/0471140864.ps1109s58"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/full-xml/10.1002/0471140864.ps1109s58"}},{"identifier":{"@type":"URI","@value":"https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/0471140864.ps1109s58"}}],"dc:title":[{"@value":"Amino Acid Analysis"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>Amino acid analysis is used to determine the amino acid content of amino acid–, peptide‐ and protein‐containing samples. With minor exceptions, proteins are long linear polymers of amino acids connected to each other via peptide bonds. The first step of amino acid analysis involves hydrolyzing these peptide bonds. The liberated amino acids are then separated, detected, and quantified. The method was first developed by Moore, Stein and coworkers in the 1950s using HCl acid hydrolysis, and, despite considerable effort by many workers, the basic methodology remains relatively unchanged. This unit provides an overview and strategic planning for amino acid analysis, discussing a range of methodologies and issues. In addition, several common methods used for analysis of <jats:sc>L</jats:sc>‐amino acids are described in detail, including: HCl acid hydrolysis, performic acid oxidation for methionine and cysteine analysis, base hydrolysis for tryptophan analysis, analysis of free amino acids, and analysis of reactive lysine. <jats:italic>Curr. Protoc. Protein Sci</jats:italic>. 58:11.9.1‐11.9.37. © 2009 by John Wiley & Sons, Inc.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262943461494528","@type":"Researcher","foaf:name":[{"@value":"Shane M. Rutherfurd"}],"jpcoar:affiliationName":[{"@value":"Riddet Institute, Massey University  Palmerston North New Zealand"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943461494529","@type":"Researcher","foaf:name":[{"@value":"G. Sarwar Gilani"}],"jpcoar:affiliationName":[{"@value":"Health Canada, Nutrition Research Division, Food Directorate, Health Products and Food Branch, Ottawa  Ontario Canada"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"19343655"},{"@type":"EISSN","@value":"19343663"}],"prism:publicationName":[{"@value":"Current Protocols in Protein Science"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2009-11","prism:volume":"58","prism:number":"1","prism:startingPage":"11.9.1"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2F0471140864.ps1109s58"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/0471140864.ps1109s58"},{"@id":"https://onlinelibrary.wiley.com/doi/full-xml/10.1002/0471140864.ps1109s58"},{"@id":"https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/0471140864.ps1109s58"}],"createdAt":"2009-11-23","modifiedAt":"2023-08-19","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050019302707015168","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Alteration and Stability of Complex Macromolecular Amino Acid Precursors in Hydrothermal Environments"}]},{"@id":"https://cir.nii.ac.jp/crid/1050581224891343872","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Gamma-Ray-Induced Amino Acid Formation in Aqueous Small Bodies in the Early Solar System"}]},{"@id":"https://cir.nii.ac.jp/crid/1360853567708206592","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Synthesis of Organic Matter in Aqueous Environments Simulating Small Bodies in the Solar System and the Effects of Minerals on Amino Acid Formation"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205736016000","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Amino acid analysis of sub-picomolar amounts of proteins by precolumn fluorescence derivatization with 6-aminoquinolyl-<i>N</i>-hydroxysuccinimidyl carbamate"},{"@value":"Amino acid analysis of sub-Picomolar amounts of proteins by precolumn fluorescence derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1002/0471140864.ps1109s58"},{"@type":"CROSSREF","@value":"10.1007/s11084-020-09593-x_references_DOI_TxBonq9MNPy8Ns5JknBcBCi1dCs"},{"@type":"CROSSREF","@value":"10.3390/life11010032_references_DOI_TxBonq9MNPy8Ns5JknBcBCi1dCs"},{"@type":"CROSSREF","@value":"10.1021/acscentsci.2c00588_references_DOI_TxBonq9MNPy8Ns5JknBcBCi1dCs"},{"@type":"CROSSREF","@value":"10.5582/bst.2011.v5.6.231_references_DOI_TxBonq9MNPy8Ns5JknBcBCi1dCs"}]}