{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262943547231104.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1101/gad.11.18.2347"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1101/gad.11.18.2347"}}],"dc:title":[{"@value":"PARP is important for genomic stability but dispensable in apoptosis"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Mice lacking the gene encoding poly(ADP-ribosyl) transferase (PARP or ADPRT) display no phenotypic abnormalities, although aged mice are susceptible to epidermal hyperplasia and obesity in a mixed genetic background. Whereas embryonic fibroblasts lacking PARP exhibit normal DNA excision repair, they grow more slowly in vitro. Here we investigated the putative roles of PARP in cell proliferation, cell death, radiosensitivity, and DNA recombination, as well as chromosomal stability. We show that the proliferation deficiency in vitro and in vivo is most likely caused by a hypersensitive response to environmental stress. Although PARP is specifically cleaved during apoptosis, cells lacking this molecule apoptosed normally in response to treatment with anti-Fas, tumor neurosis factor α, γ-irradiation, and dexamethasone, indicating that PARP is dispensable in apoptosis and that PARP−/− thymocytes are not hypersensitive to ionizing radiation. Furthermore, the capacity of mutant cells to carry out immunoglobulin class switching and<jats:italic>V(D)J</jats:italic>recombination is normal. Finally, primary PARP mutant fibroblasts and splenocytes exhibited an elevated frequency of spontaneous sister chromatid exchanges and elevated micronuclei formation after treatment with genotoxic agents, establishing an important role for PARP in the maintenance of genomic integrity.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262943547231104","@type":"Researcher","foaf:name":[{"@value":"Zhao-Qi Wang"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943547231109","@type":"Researcher","foaf:name":[{"@value":"Laura Stingl"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943547231107","@type":"Researcher","foaf:name":[{"@value":"Ciaran Morrison"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943547231105","@type":"Researcher","foaf:name":[{"@value":"Michael Jantsch"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943547231108","@type":"Researcher","foaf:name":[{"@value":"Marek Los"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943547231110","@type":"Researcher","foaf:name":[{"@value":"Klaus Schulze-Osthoff"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943547231106","@type":"Researcher","foaf:name":[{"@value":"Erwin F. Wagner"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"08909369"},{"@type":"EISSN","@value":"15495477"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/08909369"}],"prism:publicationName":[{"@value":"Genes & Development"}],"dc:publisher":[{"@value":"Cold Spring Harbor Laboratory"}],"prism:publicationDate":"1997-09-15","prism:volume":"11","prism:number":"18","prism:startingPage":"2347","prism:endingPage":"2358"},"reviewed":"false","url":[{"@id":"https://syndication.highwire.org/content/doi/10.1101/gad.11.18.2347"}],"createdAt":"2008-02-20","modifiedAt":"2023-05-16","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050287297263975680","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Spontaneous Development of Dental Dysplasia in Aged Parp-1 Knockout Mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004237102748416","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Characterization of<i>in vitro</i>radiosensitization in mammalian cells using biomathematical modelling: implications for hypofractionated radiotherapy with a combined modality approach"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285711762830976","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Comprehensive predictions of target proteins based on protein-chemical interaction using virtual screening and experimental verifications"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204145500416","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Poly(ADP-ribose) polymerase-1 gene in human tumor cell lines: Its expression and structural alteration"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204146329728","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Lack of altered frequency of sister-chromatid exchanges in poly(ADP-ribose) glycohydrolase-deficient mouse ES cells treated with methylmethanesulfonate."}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204146335744","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Impairment in S-phase entry of splenocytes of Parp-1 knockout mice."}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1101/gad.11.18.2347"},{"@type":"CROSSREF","@value":"10.1259/bjr.20150724_references_DOI_EGkUl7xC1NFkg0E5Xrd2brKGdok"},{"@type":"CROSSREF","@value":"10.1186/1472-6769-12-2_references_DOI_EGkUl7xC1NFkg0E5Xrd2brKGdok"},{"@type":"CROSSREF","@value":"10.2183/pjab.79b.248_references_DOI_EGkUl7xC1NFkg0E5Xrd2brKGdok"},{"@type":"CROSSREF","@value":"10.2183/pjab.79b.305_references_DOI_EGkUl7xC1NFkg0E5Xrd2brKGdok"},{"@type":"CROSSREF","@value":"10.3390/cells8101157_references_DOI_EGkUl7xC1NFkg0E5Xrd2brKGdok"},{"@type":"CROSSREF","@value":"10.2183/pjab.80.114_references_DOI_EGkUl7xC1NFkg0E5Xrd2brKGdok"}]}