A new flow cytometry method enabling rapid purification of fossil pollen from terrestrial sediments for <scp>AMS</scp> radiocarbon dating

DOI Web Site Web Site 被引用文献5件
  • RICHARD K. TENNANT
    Biosciences, College of Life and Environmental Sciences University of Exeter Exeter UK
  • RICHARD T. JONES
    Geography, College of Life and Environmental Sciences University of Exeter Exeter UK
  • FIONA BROCK
    Oxford Radiocarbon Accelerator Unit, Research Laboratory for Archaeology and the History of Art University of Oxford Oxford UK
  • CHARLOTTE COOK
    Climate Change Research Centre and School of Biological, Earth and Environmental Sciences University of New South Wales Sydney, NSW Australia
  • CHRIS S. M. TURNEY
    Climate Change Research Centre and School of Biological, Earth and Environmental Sciences University of New South Wales Sydney, NSW Australia
  • JOHN LOVE
    Biosciences, College of Life and Environmental Sciences University of Exeter Exeter UK
  • ROB LEE
    Biosciences, College of Life and Environmental Sciences University of Exeter Exeter UK

書誌事項

公開日
2013-03
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1002/jqs.2606
公開者
Wiley

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説明

<jats:title>ABSTRACT</jats:title><jats:sec><jats:label/><jats:p>Radiocarbon (<jats:sup>14</jats:sup>C) accelerator mass spectrometry (AMS) dating has played a significant role towards improving our understanding of the timing of events and rates of change in archaeological and environmental records over the last 50 000 years. Although it is not always possible to find suitable macrofossils for <jats:sup>14</jats:sup>C dating, microfossils, notably plant pollen, are a viable alternative. Obtaining preserved pollen samples of known provenance and of sufficient quantity for dating by <jats:sup>14</jats:sup>C AMS is, however, challenging because of time‐consuming methods of extraction and purification and possible contamination from other organic material. Here we report a new, rapid and straightforward method using flow cytometry (FCM) to distinguish, sort and collect sufficient quantities of fossil pollen with minimal contamination from lake sediments for radiocarbon dating. Using this approach, we demonstrate <jats:sup>14</jats:sup>C AMS ages that date back to at least 40 ka BP. While future work may be required to refine purification methodologies for different sample types and to precisely quantify the dating limit of this approach, FCM dating of microfossils shows considerable promise in generating robust geochronological frameworks for sequences that have previously proved problematic. Copyright © 2013 John Wiley & Sons, Ltd.</jats:p></jats:sec>

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