{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262943675575296.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/jqs.2606"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjqs.2606"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/jqs.2606"}}],"dc:title":[{"@value":"A new flow cytometry method enabling rapid purification of fossil pollen from terrestrial sediments for <scp>AMS</scp> radiocarbon dating"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title><jats:sec><jats:label/><jats:p>Radiocarbon (<jats:sup>14</jats:sup>C) accelerator mass spectrometry (AMS) dating has played a significant role towards improving our understanding of the timing of events and rates of change in archaeological and environmental records over the last 50 000 years. Although it is not always possible to find suitable macrofossils for <jats:sup>14</jats:sup>C dating, microfossils, notably plant pollen, are a viable alternative. Obtaining preserved pollen samples of known provenance and of sufficient quantity for dating by <jats:sup>14</jats:sup>C AMS is, however, challenging because of time‐consuming methods of extraction and purification and possible contamination from other organic material. Here we report a new, rapid and straightforward method using flow cytometry (FCM) to distinguish, sort and collect sufficient quantities of fossil pollen with minimal contamination from lake sediments for radiocarbon dating. Using this approach, we demonstrate <jats:sup>14</jats:sup>C AMS ages that date back to at least 40 ka BP. While future work may be required to refine purification methodologies for different sample types and to precisely quantify the dating limit of this approach, FCM dating of microfossils shows considerable promise in generating robust geochronological frameworks for sequences that have previously proved problematic. Copyright © 2013 John Wiley & Sons, Ltd.</jats:p></jats:sec>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262943675575298","@type":"Researcher","foaf:name":[{"@value":"RICHARD K. TENNANT"}],"jpcoar:affiliationName":[{"@value":"Biosciences, College of Life and Environmental Sciences University of Exeter Exeter UK"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943675575296","@type":"Researcher","foaf:name":[{"@value":"RICHARD T. JONES"}],"jpcoar:affiliationName":[{"@value":"Geography, College of Life and Environmental Sciences University of Exeter Exeter UK"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943675575426","@type":"Researcher","foaf:name":[{"@value":"FIONA BROCK"}],"jpcoar:affiliationName":[{"@value":"Oxford Radiocarbon Accelerator Unit, Research Laboratory for Archaeology and the History of Art University of Oxford Oxford UK"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943675575297","@type":"Researcher","foaf:name":[{"@value":"CHARLOTTE COOK"}],"jpcoar:affiliationName":[{"@value":"Climate Change Research Centre and School of Biological, Earth and Environmental Sciences University of New South Wales Sydney, NSW Australia"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943675575424","@type":"Researcher","foaf:name":[{"@value":"CHRIS S. M. TURNEY"}],"jpcoar:affiliationName":[{"@value":"Climate Change Research Centre and School of Biological, Earth and Environmental Sciences University of New South Wales Sydney, NSW Australia"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943675575427","@type":"Researcher","foaf:name":[{"@value":"JOHN LOVE"}],"jpcoar:affiliationName":[{"@value":"Biosciences, College of Life and Environmental Sciences University of Exeter Exeter UK"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943675575425","@type":"Researcher","foaf:name":[{"@value":"ROB LEE"}],"jpcoar:affiliationName":[{"@value":"Biosciences, College of Life and Environmental Sciences University of Exeter Exeter UK"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"02678179"},{"@type":"EISSN","@value":"10991417"}],"prism:publicationName":[{"@value":"Journal of Quaternary Science"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2013-03","prism:volume":"28","prism:number":"3","prism:startingPage":"229","prism:endingPage":"236"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjqs.2606"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/jqs.2606"}],"createdAt":"2013-03-02","modifiedAt":"2023-10-30","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050577541485178752","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"A simple method for taxon-specific purification of diatom frustules from ocean sediments using a cell sorter"},{"@value":"A simple method for taxon-specific purifcation of diatom frustules from ocean sediments using a cell sorter"}]},{"@id":"https://cir.nii.ac.jp/crid/1360005516799846528","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Dating tsunami deposits: Present knowledge and challenges"}]},{"@id":"https://cir.nii.ac.jp/crid/1360009142495482368","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Breakthrough in purification of fossil pollen for dating of sediments by a new large-particle on-chip sorter"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013168754180224","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Extraction method for fossil pollen grains using a cell sorter suitable for routine 14C dating"}]},{"@id":"https://cir.nii.ac.jp/crid/1360306505602437760","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Development of an automated extraction and radiocarbon dating method for fossil pollen deposited in lake Motosu, Japan"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1002/jqs.2606"},{"@type":"CROSSREF","@value":"10.1126/sciadv.abe7327_references_DOI_IOQpIH1KcccjpSZx5qMJTJL2yLv"},{"@type":"CROSSREF","@value":"10.1016/j.quascirev.2021.107236_references_DOI_IOQpIH1KcccjpSZx5qMJTJL2yLv"},{"@type":"CROSSREF","@value":"10.1186/s40645-023-00543-5_references_DOI_IOQpIH1KcccjpSZx5qMJTJL2yLv"},{"@type":"CROSSREF","@value":"10.1016/j.earscirev.2019.102971_references_DOI_IOQpIH1KcccjpSZx5qMJTJL2yLv"},{"@type":"CROSSREF","@value":"10.1016/j.qsa.2024.100207_references_DOI_IOQpIH1KcccjpSZx5qMJTJL2yLv"}]}