{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262943712993152.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.4049/jimmunol.166.8.4915"}},{"identifier":{"@type":"URI","@value":"https://academic.oup.com/jimmunol/article-pdf/166/8/4915/62530293/4915.pdf"}}],"dc:title":[{"@value":"IL-12 Alone and in Synergy with IL-18 Inhibits Osteoclast Formation In Vitro"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n                  <jats:p>IL-12, like IL-18, was shown to potently inhibit osteoclast formation in cultures of cocultures of murine osteoblast and spleen cells, as well as in adult spleen cells treated with M-CSF and receptor activator of NF-κB ligand (RANKL). Neither IL-12 nor IL-18 was able to inhibit RANKL-induced osteoclast formation in cultured RAW264.7 cells, demonstrating that IL-12, like IL-18, was unable to act directly on osteoclastic precursors. IL-12, like IL-18, was found to act by T cells, since depletion of T cells from the adult spleen cell cultures ablated the inhibitory action of IL-12 and addition of either CD4 or CD8 T cells from C57BL/6 mice to RANKL-stimulated RAW264.7 cultures permitted IL-12 or IL-18 to be inhibitory. Additionally, IL-12 was still able to inhibit osteoclast formation in cocultures with osteoblasts and spleen cells from either GM-CSF R−/− mice or IFN-γ R−/− mice, indicating that neither GM-CSF nor IFN-γ was mediating osteoclast inhibition in these cultures. Combined, IL-18 and IL-12 synergistically inhibited osteoclast formation at concentrations 20- to 1000-fold less, respectively, than when added individually. A candidate inhibitor could not be demonstrated using neutralizing Abs to IL-4, IL-10, or IL-13 or from mRNA expression profiles among known cytokine inhibitors of osteoclastogenesis in response to IL-12 and IL-18 treatment, although the unknown inhibitory molecule was determined to be secreted from T cells.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380307540997789184","@type":"Researcher","foaf:name":[{"@value":"Nicole J Horwood"}],"jpcoar:affiliationName":[{"@value":"St. Vincent’s Institute of Medical Research , Fitzroy, Victoria ,"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943712993153","@type":"Researcher","foaf:name":[{"@value":"Jan Elliott"}],"jpcoar:affiliationName":[{"@value":"St. Vincent’s Institute of Medical Research , Fitzroy, Victoria ,"}]},{"@id":"https://cir.nii.ac.jp/crid/1380307540997789186","@type":"Researcher","foaf:name":[{"@value":"T John Martin"}],"jpcoar:affiliationName":[{"@value":"St. Vincent’s Institute of Medical Research , Fitzroy, Victoria ,"}]},{"@id":"https://cir.nii.ac.jp/crid/1380307540997789185","@type":"Researcher","foaf:name":[{"@value":"Matthew T Gillespie"}],"jpcoar:affiliationName":[{"@value":"St. Vincent’s Institute of Medical Research , Fitzroy, Victoria ,"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00221767"},{"@type":"EISSN","@value":"15506606"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/00221767"}],"prism:publicationName":[{"@value":"The Journal of Immunology"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2001-04","prism:volume":"166","prism:number":"8","prism:startingPage":"4915","prism:endingPage":"4921"},"reviewed":"false","dc:rights":["https://academic.oup.com/pages/standard-publication-reuse-rights"],"url":[{"@id":"https://academic.oup.com/jimmunol/article-pdf/166/8/4915/62530293/4915.pdf"}],"createdAt":"2014-04-20","modifiedAt":"2026-02-02","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050005822263500928","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Immunological Reaction in TNF-α-Mediated Osteoclast Formation and Bone Resorption In Vitro and In Vivo"}]},{"@id":"https://cir.nii.ac.jp/crid/1050005822263661824","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"IL-12 Inhibits Lipopolysaccharide Stimulated Osteoclastogenesis in Mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1050568772252075776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effect of Cytokines on Osteoclast Formation and Bone Resorption during Mechanical Force Loading of the Periodontal Membrane"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004231180758016","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"IL-12- and IL-18-Mediated, Nitric Oxide-Induced Apoptosis in TNF-α-Mediated Osteoclastogenesis of Bone Marrow Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004231982364928","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Inhibitory effects of IL-12 on experimental tooth movement and root resorption in mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004232214152320","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"IFN-γ directly inhibits TNF-α-induced osteoclastogenesis in vitro and in vivo and induces apoptosis mediated by Fas/Fas ligand interactions"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285706320991616","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"R848, a toll-like receptor 7 agonist, inhibits osteoclast differentiation but not survival or bone-resorbing function of mature osteoclasts"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848659409996800","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Inhibitory Effect of Interferon-γ on Experimental Tooth Movement in Mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1360853567585675136","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Osteocyte-Related Cytokines Regulate Osteoclast Formation and Bone Resorption"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861707149862272","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effect of T cells on bone"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679392705792","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"歯の移動とメカニカルストレス―骨細胞の働きと骨免疫因子―"},{"@language":"en","@value":"Tooth movement and mechanical stress -Role of osteocytes and osteoimmune factor-"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679602755072","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Suppressive Effect of Kanzo-bushi-to, a Kampo Medicine, on Collagen-Induced Arthritis"},{"@value":"Suppressive Effect of Kanzo-bushi-to, a Kampo Medicine, on Collagell-Induced Arthritis"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.4049/jimmunol.166.8.4915"},{"@type":"CROSSREF","@value":"10.1155/2014/617032_references_DOI_QIv1bpA171YO3BM2dt6aUzzZBou"},{"@type":"CROSSREF","@value":"10.1007/s00223-011-9494-0_references_DOI_QIv1bpA171YO3BM2dt6aUzzZBou"},{"@type":"CROSSREF","@value":"10.1016/j.archoralbio.2011.07.006_references_DOI_QIv1bpA171YO3BM2dt6aUzzZBou"},{"@type":"CROSSREF","@value":"10.1155/2013/181849_references_DOI_QIv1bpA171YO3BM2dt6aUzzZBou"},{"@type":"CROSSREF","@value":"10.1007/s10616-012-9442-5_references_DOI_QIv1bpA171YO3BM2dt6aUzzZBou"},{"@type":"CROSSREF","@value":"10.1089/jir.2011.0124_references_DOI_QIv1bpA171YO3BM2dt6aUzzZBou"},{"@type":"CROSSREF","@value":"10.1155/2015/214878_references_DOI_QIv1bpA171YO3BM2dt6aUzzZBou"},{"@type":"CROSSREF","@value":"10.2329/perio.58.213_references_DOI_QIv1bpA171YO3BM2dt6aUzzZBou"},{"@type":"CROSSREF","@value":"10.3390/ijms21145169_references_DOI_QIv1bpA171YO3BM2dt6aUzzZBou"},{"@type":"CROSSREF","@value":"10.1016/j.bone.2023.116675_references_DOI_QIv1bpA171YO3BM2dt6aUzzZBou"},{"@type":"CROSSREF","@value":"10.1248/bpb.27.1406_references_DOI_QIv1bpA171YO3BM2dt6aUzzZBou"},{"@type":"CROSSREF","@value":"10.1016/j.imlet.2011.02.017_references_DOI_QIv1bpA171YO3BM2dt6aUzzZBou"}]}