{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262943863131008.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1021/tx300259x"}},{"identifier":{"@type":"URI","@value":"https://pubs.acs.org/doi/pdf/10.1021/tx300259x"}},{"identifier":{"@type":"PMID","@value":"22804710"}}],"dc:title":[{"@value":"Mutagenic Bypass of 8-Oxo-7,8-dihydroguanine (8-Hydroxyguanine) by DNA Polymerase κ in Human Cells"}],"description":[{"notation":[{"@value":"The formation of 8-oxo-7,8-dihydroguanine (G(O), 8-hydroxyguanine) in DNA and in the nucleotide pool results in G:C→T:A and A:T→C:G substitution mutations, respectively, since G(O) can pair with both C and A. In this study, the role of DNA polymerase κ in the mutagenicity of G(O) was investigated, using a supF shuttle plasmid propagated in human U2OS cells. This translesion synthesis DNA polymerase was knocked down by siRNA, and plasmid DNAs containing G(O):C and G(O):A pairs were transfected into the knock-down cells. The supF plasmid DNAs replicated in the cells were then introduced into Escherichia coli. Mutation analyses indicated that the knock-down of DNA polymerase κ by siRNA decreased the frequency of G:C→T:A mutation caused by G(O):C, although no effects of the DNA polymerase κ reduction were observed for the A:T→C:G substitution induced by G(O):A. These results suggested that DNA polymerase κ is involved in the mutagenic bypass of G(O) in living human cells, when the damaged base is generated by direct DNA oxidation."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262943863131009","@type":"Researcher","foaf:name":[{"@value":"Hiroyuki Kamiya"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Science and Engineering, Ehime University, 2-5 Bunkyo-cho, Matsuyama 790-8577,\rJapan"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943863131008","@type":"Researcher","foaf:name":[{"@value":"Masahiro Kurokawa"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Science and Engineering, Ehime University, 2-5 Bunkyo-cho, Matsuyama 790-8577,\rJapan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0893228X"},{"@type":"EISSN","@value":"15205010"}],"prism:publicationName":[{"@value":"Chemical Research in Toxicology"}],"dc:publisher":[{"@value":"American Chemical Society (ACS)"}],"prism:publicationDate":"2012-07-30","prism:volume":"25","prism:number":"8","prism:startingPage":"1771","prism:endingPage":"1776"},"reviewed":"false","url":[{"@id":"https://pubs.acs.org/doi/pdf/10.1021/tx300259x"}],"createdAt":"2012-07-18","modifiedAt":"2023-04-16","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Replication","dc:title":"DNA Replication"},{"@id":"https://cir.nii.ac.jp/all?q=Guanine","dc:title":"Guanine"},{"@id":"https://cir.nii.ac.jp/all?q=DNA","dc:title":"DNA"},{"@id":"https://cir.nii.ac.jp/all?q=DNA-Directed%20DNA%20Polymerase","dc:title":"DNA-Directed DNA Polymerase"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line,%20Tumor","dc:title":"Cell Line, Tumor"},{"@id":"https://cir.nii.ac.jp/all?q=Mutation","dc:title":"Mutation"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=RNA%20Interference","dc:title":"RNA Interference"},{"@id":"https://cir.nii.ac.jp/all?q=RNA,%20Small%20Interfering","dc:title":"RNA, Small Interfering"},{"@id":"https://cir.nii.ac.jp/all?q=Base%20Pairing","dc:title":"Base Pairing"},{"@id":"https://cir.nii.ac.jp/all?q=Nucleic%20Acid%20Synthesis%20Inhibitors","dc:title":"Nucleic Acid Synthesis Inhibitors"},{"@id":"https://cir.nii.ac.jp/all?q=Plasmids","dc:title":"Plasmids"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002214542712832","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Catalytic and non‐catalytic roles of <scp>DNA</scp> polymerase κ in the protection of human cells against genotoxic stresses"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285705083970176","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"DNA polymerase kappa counteracts inflammation‐induced mutagenesis in multiple organs of mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285708230945920","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Action-at-a-Distance Mutagenesis Induced by Oxidized Guanine in Werner Syndrome Protein-Reduced Human Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285708959610368","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Impact of Ribonucleotide Backbone on Translesion Synthesis and Repair of 7,8-Dihydro-8-oxoguanine"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567180060660864","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Limited ability of DNA polymerase kappa to suppress benzo[<i>a</i>]pyrene‐induced genotoxicity in vivo"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567184619914368","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Mutations induced by 8-oxo-7,8-dihydroguanine in WRN- and DNA polymerase λ-double knockdown cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205257244032","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"DNA Polymerase λ Promotes Mutagenesis Induced by 8-Oxo-7,8-dihydroguanine (8-hydroxyguanine) Paired with Adenine"},{"@value":"DNA Polymerase ^|^lambda; Promotes Mutagenesis Induced by 8-Oxo-7,8-dihydroguanine (8-hydroxyguanine) Paired with Adenine"}]},{"@id":"https://cir.nii.ac.jp/crid/2051151841912727552","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Mutations induced by 8-hydroxyguanine (8-oxo-7,8-dihydroguanine), a representative oxidized base, in mammalian cells"}]},{"@id":"https://cir.nii.ac.jp/crid/2051714791866149504","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"DNA polymerase kappa protects human cells against MMC-induced genotoxicity through error-free translesion DNA synthesis"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1021/tx300259x"},{"@type":"OPENAIRE","@value":"doi_dedup___::5438dbdf585a9c863718c441f710609d"},{"@type":"CROSSREF","@value":"10.1002/em.21961_references_DOI_DWMneS4r6c7s7EB4tQcW20HAJLb"},{"@type":"CROSSREF","@value":"10.1002/em.22272_references_DOI_DWMneS4r6c7s7EB4tQcW20HAJLb"},{"@type":"CROSSREF","@value":"10.1074/jbc.m116.738732_references_DOI_DWMneS4r6c7s7EB4tQcW20HAJLb"},{"@type":"CROSSREF","@value":"10.1002/em.22146_references_DOI_DWMneS4r6c7s7EB4tQcW20HAJLb"},{"@type":"CROSSREF","@value":"10.1093/mutage/gey024_references_DOI_DWMneS4r6c7s7EB4tQcW20HAJLb"},{"@type":"CROSSREF","@value":"10.3123/jemsge.2013.002_references_DOI_DWMneS4r6c7s7EB4tQcW20HAJLb"},{"@type":"CROSSREF","@value":"10.1021/tx500418m_references_DOI_DWMneS4r6c7s7EB4tQcW20HAJLb"},{"@type":"CROSSREF","@value":"10.1186/s41021-016-0051-y_references_DOI_DWMneS4r6c7s7EB4tQcW20HAJLb"},{"@type":"CROSSREF","@value":"10.1186/s41021-016-0067-3_references_DOI_DWMneS4r6c7s7EB4tQcW20HAJLb"}]}