{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262943933887360.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.2336103100"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.2336103100"}},{"identifier":{"@type":"NAID","@value":"30016238539"}}],"dc:title":[{"@value":"Wnk1 kinase deficiency lowers blood pressure in mice: A gene-trap screen to identify potential targets for therapeutic intervention"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>\n            The availability of both the mouse and human genome sequences allows for the systematic discovery of human gene function through the use of the mouse as a model system. To accelerate the genetic determination of gene function, we have developed a sequence-tagged gene-trap library of >270,000 mouse embryonic stem cell clones representing mutations in ≈60% of mammalian genes. Through the generation and phenotypic analysis of knockout mice from this resource, we are undertaking a functional screen to identify genes regulating physiological parameters such as blood pressure. As part of this screen, mice deficient for the\n            <jats:italic>Wnk1</jats:italic>\n            kinase gene were generated and analyzed. Genetic studies in humans have shown that large intronic deletions in\n            <jats:italic>WNK1</jats:italic>\n            lead to its overexpression and are responsible for pseudohypoaldosteronism type II, an autosomal dominant disorder characterized by hypertension, increased renal salt reabsorption, and impaired K\n            <jats:sup>+</jats:sup>\n            and H\n            <jats:sup>+</jats:sup>\n            excretion. Consistent with the human genetic studies,\n            <jats:italic>Wnk1</jats:italic>\n            heterozygous mice displayed a significant decrease in blood pressure. Mice homozygous for the\n            <jats:italic>Wnk1</jats:italic>\n            mutation died during embryonic development before day 13 of gestation. These results demonstrate that\n            <jats:italic>Wnk1</jats:italic>\n            is a regulator of blood pressure critical for development and illustrate the utility of a functional screen driven by a sequence-based mutagenesis approach.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380013170750364829","@type":"Researcher","foaf:name":[{"@value":"Brian P. Zambrowicz"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364807","@type":"Researcher","foaf:name":[{"@value":"Alejandro Abuin"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364802","@type":"Researcher","foaf:name":[{"@value":"Ramiro Ramirez-Solis"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364800","@type":"Researcher","foaf:name":[{"@value":"Lizabeth J. Richter"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364806","@type":"Researcher","foaf:name":[{"@value":"James Piggott"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364828","@type":"Researcher","foaf:name":[{"@value":"Hector BeltrandelRio"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364812","@type":"Researcher","foaf:name":[{"@value":"Eric C. Buxton"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364825","@type":"Researcher","foaf:name":[{"@value":"Joel Edwards"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364818","@type":"Researcher","foaf:name":[{"@value":"Rick A. Finch"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364808","@type":"Researcher","foaf:name":[{"@value":"Carl J. Friddle"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364810","@type":"Researcher","foaf:name":[{"@value":"Anupma Gupta"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364817","@type":"Researcher","foaf:name":[{"@value":"Gwenn Hansen"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364827","@type":"Researcher","foaf:name":[{"@value":"Yi Hu"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364815","@type":"Researcher","foaf:name":[{"@value":"Wenhu Huang"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364826","@type":"Researcher","foaf:name":[{"@value":"Crystal Jaing"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364816","@type":"Researcher","foaf:name":[{"@value":"Billie Wayne Key"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364819","@type":"Researcher","foaf:name":[{"@value":"Peter Kipp"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364823","@type":"Researcher","foaf:name":[{"@value":"Buckley Kohlhauff"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364803","@type":"Researcher","foaf:name":[{"@value":"Zhi-Qing Ma"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364813","@type":"Researcher","foaf:name":[{"@value":"Diane Markesich"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364824","@type":"Researcher","foaf:name":[{"@value":"Robert Payne"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364675","@type":"Researcher","foaf:name":[{"@value":"David G. Potter"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364673","@type":"Researcher","foaf:name":[{"@value":"Ny Qian"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364801","@type":"Researcher","foaf:name":[{"@value":"Joseph Shaw"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364674","@type":"Researcher","foaf:name":[{"@value":"Jeff Schrick"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364820","@type":"Researcher","foaf:name":[{"@value":"Zheng-Zheng Shi"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364821","@type":"Researcher","foaf:name":[{"@value":"Mary Jean Sparks"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364804","@type":"Researcher","foaf:name":[{"@value":"Isaac Van Sligtenhorst"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364672","@type":"Researcher","foaf:name":[{"@value":"Peter Vogel"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364809","@type":"Researcher","foaf:name":[{"@value":"Wade Walke"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364814","@type":"Researcher","foaf:name":[{"@value":"Nianhua Xu"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364822","@type":"Researcher","foaf:name":[{"@value":"Qichao Zhu"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364805","@type":"Researcher","foaf:name":[{"@value":"Christophe Person"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170750364811","@type":"Researcher","foaf:name":[{"@value":"Arthur T. Sands"}],"jpcoar:affiliationName":[{"@value":"Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00278424"},{"@type":"EISSN","@value":"10916490"}],"prism:publicationName":[{"@value":"Proceedings of the National Academy of Sciences"}],"dc:publisher":[{"@value":"Proceedings of the National Academy of Sciences"}],"prism:publicationDate":"2003-11-10","prism:volume":"100","prism:number":"24","prism:startingPage":"14109","prism:endingPage":"14114"},"reviewed":"false","url":[{"@id":"https://pnas.org/doi/pdf/10.1073/pnas.2336103100"}],"createdAt":"2003-11-25","modifiedAt":"2022-04-26","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002219065060736","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Impaired Processing of Prohormones in Secretogranin III–Null Mice Causes Maladaptation to an Inadequate Diet and Stress"}]},{"@id":"https://cir.nii.ac.jp/crid/1360025430643238400","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Familial Hyperkalemic Hypertension"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283694068020736","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A minor role of WNK3 in regulating phosphorylation of renal NKCC2 and NCC co-transporters <i>in vivo</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285710529257984","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Transforming growth factor‐β‐stimulated clone‐22 is a negative‐feedback regulator of Ras / Raf signaling: Implications for tumorigenesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565169327222656","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A Novel Protein, CHRONO, Functions as a Core Component of the Mammalian Circadian Clock"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567187468698880","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"WNK Signaling Is Involved in Neural Development via Lhx8/Awh Expression"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567190494667904","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Autophagy linked FYVE (Alfy/WDFY3) is required for establishing neuronal connectivity in the mammalian brain"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848660450724224","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"<scp>WNK</scp>4 is an essential effector of anterior formation in <scp>FGF</scp> signaling"}]},{"@id":"https://cir.nii.ac.jp/crid/1360853567600428544","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Expression Pattern of the <i>LacZ</i> Reporter in Secretogranin III Gene-trapped Mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679604760448","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Modulation and Compensation of the mRNA Expression of Energy Related Transporters in the Brain of Glucose Transporter 1-Deficient Mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1571417125470609536","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"遺伝子異常による二次性高血圧"},{"@language":"en","@value":"Secondary hypertension due to genetic abnormality"}]},{"@id":"https://cir.nii.ac.jp/crid/2051433317027251840","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effect of heterozygous deletion of WNK1 on the WNK-OSR1/SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood vessels"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1073/pnas.2336103100"},{"@type":"CIA","@value":"30016238539"},{"@type":"CROSSREF","@value":"10.1210/en.2017-00636_references_DOI_AvDb5U0sKADbV6vQpCvHfbdiaiu"},{"@type":"CROSSREF","@value":"10.1002/cphy.c240004_references_DOI_AvDb5U0sKADbV6vQpCvHfbdiaiu"},{"@type":"CROSSREF","@value":"10.1111/j.1349-7006.2011.02108.x_references_DOI_AvDb5U0sKADbV6vQpCvHfbdiaiu"},{"@type":"CROSSREF","@value":"10.1371/journal.pbio.1001839_references_DOI_AvDb5U0sKADbV6vQpCvHfbdiaiu"},{"@type":"CROSSREF","@value":"10.1371/journal.pone.0055301_references_DOI_AvDb5U0sKADbV6vQpCvHfbdiaiu"},{"@type":"CROSSREF","@value":"10.7554/elife.14810_references_DOI_AvDb5U0sKADbV6vQpCvHfbdiaiu"},{"@type":"CROSSREF","@value":"10.1007/s10157-012-0590-x_references_DOI_AvDb5U0sKADbV6vQpCvHfbdiaiu"},{"@type":"CROSSREF","@value":"10.1111/gtc.12048_references_DOI_AvDb5U0sKADbV6vQpCvHfbdiaiu"},{"@type":"CROSSREF","@value":"10.1242/bio.2011048_references_DOI_AvDb5U0sKADbV6vQpCvHfbdiaiu"},{"@type":"CROSSREF","@value":"10.1369/0022155421996845_references_DOI_AvDb5U0sKADbV6vQpCvHfbdiaiu"},{"@type":"CROSSREF","@value":"10.1248/bpb.29.1587_references_DOI_AvDb5U0sKADbV6vQpCvHfbdiaiu"}]}