{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262943942954880.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.4049/jimmunol.181.8.5537"}},{"identifier":{"@type":"URI","@value":"https://academic.oup.com/jimmunol/article-pdf/181/8/5537/62581460/7872.pdf"}}],"dc:title":[{"@value":"Binding of Complement Inhibitor C4b-Binding Protein Contributes to Serum Resistance of\n                    <i>Porphyromonas gingivalis</i>"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n                  <jats:p>The periodontal pathogen Porphyromonas gingivalis is highly resistant to the bactericidal activity of human complement, which is present in the gingival crevicular fluid at 70% of serum concentration. All thirteen clinical and laboratory P. gingivalis strains tested were able to capture the human complement inhibitor C4b-binding protein (C4BP), which may contribute to their serum resistance. Accordingly, in serum deficient of C4BP, it was found that significantly more terminal complement component C9 was deposited on P. gingivalis. Moreover, using purified proteins and various isogenic mutants, we found that the cysteine protease high molecular weight arginine-gingipain A (HRgpA) is a crucial C4BP ligand on the bacterial surface. Binding of C4BP to P. gingivalis appears to be localized to two binding sites: on the complement control protein 1 domain and complement control protein 6 and 7 domains of the α-chains. Furthermore, the bacterial binding of C4BP was found to increase with time of culture and a particularly strong binding was observed for large aggregates of bacteria that formed during culture on solid blood agar medium. Taken together, gingipains appear to be a very significant virulence factor not only destroying complement due to proteolytic degradation as we have shown previously, but was also inhibiting complement activation due to their ability to bind the complement inhibitor C4BP.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262943942954887","@type":"Researcher","foaf:name":[{"@value":"Michal Potempa"}],"jpcoar:affiliationName":[{"@value":"Department of Laboratory Medicine, Section of Medical Protein Chemistry, University Hospital Malmö, Lund University , Malmö,"},{"@value":"Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University , Krakow,"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943942954880","@type":"Researcher","foaf:name":[{"@value":"Jan Potempa"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University , Krakow,"},{"@value":"Department of Biochemistry and Molecular Biology, University of Georgia , Athens, GA 30602"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943942954886","@type":"Researcher","foaf:name":[{"@value":"Marcin Okroj"}],"jpcoar:affiliationName":[{"@value":"Department of Laboratory Medicine, Section of Medical Protein Chemistry, University Hospital Malmö, Lund University , Malmö,"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943942954882","@type":"Researcher","foaf:name":[{"@value":"Katarzyna Popadiak"}],"jpcoar:affiliationName":[{"@value":"Department of Laboratory Medicine, Section of Medical Protein Chemistry, University Hospital Malmö, Lund University , Malmö,"},{"@value":"Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University , Krakow,"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943942954883","@type":"Researcher","foaf:name":[{"@value":"Sigrun Eick"}],"jpcoar:affiliationName":[{"@value":"Department of Medical Microbiology, University Hospital of Jena , Jena,"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943942954885","@type":"Researcher","foaf:name":[{"@value":"Ky-Anh Nguyen"}],"jpcoar:affiliationName":[{"@value":"Westmead Centre for Oral Health, Institute of Dental Research , Sydney,"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943942954884","@type":"Researcher","foaf:name":[{"@value":"Kristian Riesbeck"}],"jpcoar:affiliationName":[{"@value":"Department of Laboratory Medicine, Section of Medical Microbiology, University Hospital Malmö, Lund University , Malmö,"}]},{"@id":"https://cir.nii.ac.jp/crid/1380025803510717696","@type":"Researcher","foaf:name":[{"@value":"Anna M Blom"}],"jpcoar:affiliationName":[{"@value":"Department of Laboratory Medicine, Section of Medical Protein Chemistry, University Hospital Malmö, Lund University , Malmö,"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"15506606"},{"@type":"PISSN","@value":"00221767"}],"prism:publicationName":[{"@value":"The Journal of Immunology"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2008-10","prism:volume":"181","prism:number":"8","prism:startingPage":"5537","prism:endingPage":"5544"},"reviewed":"false","dc:rights":["https://academic.oup.com/pages/standard-publication-reuse-rights"],"url":[{"@id":"https://academic.oup.com/jimmunol/article-pdf/181/8/5537/62581460/7872.pdf"}],"createdAt":"2014-04-18","modifiedAt":"2026-01-28","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004230172775552","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Energy dispersive spectroscopy‐scanning transmission electron microscope observations of free radical production in human polymorphonuclear leukocytes phagocytosing non‐opsonized <i>Tannerella forsythia</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004230548445824","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Complement Involvement in Periodontitis: Molecular Mechanisms and Rational Therapeutic Approaches"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004235974355200","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The Surface Layer of Tannerella forsythia Contributes to Serum Resistance and Oral Bacterial Coaggregation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567187581908480","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"OmpA-like proteins of Porphyromonas gingivalis contribute to serum resistance and prevent Toll-like receptor 4-mediated host cell activation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846643220966528","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Analysis of proteins in human gingival crevicular fluid by mass spectrometry"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848661286811392","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"OmpA-Like Proteins of<i> Porphyromonas gingivalis</i> Mediate Resistance to the Antimicrobial Peptide LL-37"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680156720000","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Roles of Oral Bacteria in Cardiovascular Diseases — From Molecular Mechanisms to Clinical Cases: Preface"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.4049/jimmunol.181.8.5537"},{"@type":"CROSSREF","@value":"10.1002/jemt.22819_references_DOI_9Q9i1lz1lNGoo2MwiexNXjmP7an"},{"@type":"CROSSREF","@value":"10.1007/978-3-319-18603-0_4_references_DOI_9Q9i1lz1lNGoo2MwiexNXjmP7an"},{"@type":"CROSSREF","@value":"10.1128/iai.00983-12_references_DOI_9Q9i1lz1lNGoo2MwiexNXjmP7an"},{"@type":"CROSSREF","@value":"10.1371/journal.pone.0202791_references_DOI_9Q9i1lz1lNGoo2MwiexNXjmP7an"},{"@type":"CROSSREF","@value":"10.1111/j.1600-0765.2011.01458.x_references_DOI_9Q9i1lz1lNGoo2MwiexNXjmP7an"},{"@type":"CROSSREF","@value":"10.1155/2018/2068435_references_DOI_9Q9i1lz1lNGoo2MwiexNXjmP7an"},{"@type":"CROSSREF","@value":"10.1254/jphs.09r27fm_references_DOI_9Q9i1lz1lNGoo2MwiexNXjmP7an"}]}