{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262943967792896.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1186/s13059-015-0715-0"}},{"identifier":{"@type":"URI","@value":"https://link.springer.com/content/pdf/10.1186/s13059-015-0715-0.pdf"}},{"identifier":{"@type":"URI","@value":"https://link.springer.com/article/10.1186/s13059-015-0715-0/fulltext.html"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/content/pdf/10.1186/s13059-015-0715-0"}}],"dc:title":[{"@value":"Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n                  <jats:p>\n                    <jats:italic>Arabidopsis</jats:italic>\n                    mutants produced by constitutive overexpression of the CRISPR/Cas9 genome editing system are usually mosaics in the T1 generation. In this study, we used egg cell-specific promoters to drive the expression of\n                    <jats:italic>Cas9</jats:italic>\n                    and obtained non-mosaic T1 mutants for multiple target genes with high efficiency. Comparisons of 12 combinations of eight promoters and two terminators found that the efficiency of the egg cell-specific promoter-controlled CRISPR/Cas9 system depended on the presence of a suitable terminator, and the composite promoter generated by fusing two egg cell-specific promoters resulted in much higher efficiency of mutation in the T1 generation compared with the single promoters.\n                  </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262943967792898","@type":"Researcher","foaf:name":[{"@value":"Zhi-Ping Wang"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943967792897","@type":"Researcher","foaf:name":[{"@value":"Hui-Li Xing"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943967792901","@type":"Researcher","foaf:name":[{"@value":"Li Dong"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943967792899","@type":"Researcher","foaf:name":[{"@value":"Hai-Yan Zhang"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943967792896","@type":"Researcher","foaf:name":[{"@value":"Chun-Yan Han"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943967792900","@type":"Researcher","foaf:name":[{"@value":"Xue-Chen Wang"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262943967792902","@type":"Researcher","foaf:name":[{"@value":"Qi-Jun Chen"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"14656906"}],"prism:publicationName":[{"@value":"Genome Biology"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2015-07-21","prism:volume":"16","prism:number":"1","prism:startingPage":"144"},"reviewed":"false","dc:rights":["https://creativecommons.org/licenses/by/4.0","https://creativecommons.org/licenses/by/4.0"],"url":[{"@id":"https://link.springer.com/content/pdf/10.1186/s13059-015-0715-0.pdf"},{"@id":"https://link.springer.com/article/10.1186/s13059-015-0715-0/fulltext.html"},{"@id":"http://link.springer.com/content/pdf/10.1186/s13059-015-0715-0"}],"createdAt":"2015-07-20","modifiedAt":"2024-10-14","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050585658876533504","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants."}]},{"@id":"https://cir.nii.ac.jp/crid/1360004230485389952","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Gene Expression and Transcription Factor Binding Tests Using Mutated-Promoter Reporter Lines"}]},{"@id":"https://cir.nii.ac.jp/crid/1360015333315028224","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Genome editing approaches using reproductive cells/tissues in flowering plants"}]},{"@id":"https://cir.nii.ac.jp/crid/1360022304968946560","@type":"Article","resourceType":"preprint","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant specific motif and acts in a partially redundant manner with the tonoplast enzyme"}]},{"@id":"https://cir.nii.ac.jp/crid/1360022304971469184","@type":"Article","resourceType":"preprint","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A two-way molecular dialogue between embryo and endosperm required for seed development"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285708447248256","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The CLE9/10 secretory peptide regulates stomatal and vascular development through distinct receptors"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285708461747328","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Highly efficient heritable targeted deletions of gene clusters and non-coding regulatory regions in Arabidopsis using CRISPR/Cas9"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285709717089024","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"pKAMA-ITACHI Vectors for Highly Efficient CRISPR/Cas9-Mediated Gene Knockout in<i>Arabidopsis thaliana</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285715518328320","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Integration of light and metabolic signals for stem cell activation at the shoot apical meristem"}]},{"@id":"https://cir.nii.ac.jp/crid/1360290617904961152","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif"}]},{"@id":"https://cir.nii.ac.jp/crid/1360298757204209024","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Elongation factor 1 is a component of the <i>Arabidopsis</i> <scp>RNA</scp> polymerase <scp>II</scp> elongation complex and associates with a subset of transcribed genes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182510697984","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Germ cell regeneration-mediated, enhanced mutagenesis in the ascidian Ciona intestinalis reveals flexible germ cell formation from different somatic cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360576118812174976","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants"}]},{"@id":"https://cir.nii.ac.jp/crid/1360580232384751104","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"ROP Interactive Partners are Involved in the Control of Cell Division Patterns in Arabidopsis Leaves"}]},{"@id":"https://cir.nii.ac.jp/crid/1360588381080175488","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Evolution of interorganismal strigolactone biosynthesis in seed plants"}]},{"@id":"https://cir.nii.ac.jp/crid/1360853567665555584","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Active DNA demethylation regulates tracheary element differentiation in<i>Arabidopsis</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360857593731430272","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A Simple Heat Treatment Increases SpCas9-Mediated Mutation Efficiency in Arabidopsis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861705562644608","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A Dof-CLE circuit controls phloem organization"}]},{"@id":"https://cir.nii.ac.jp/crid/1360869855113411072","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A land plant‐specific\n                    <scp>VPS</scp>\n                    13 mediates polarized vesicle trafficking in germinating pollen"}]},{"@id":"https://cir.nii.ac.jp/crid/1361131420873829504","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A two-way molecular dialogue between embryo and endosperm is required for seed development"}]},{"@id":"https://cir.nii.ac.jp/crid/1361412893238952576","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418519274573568","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Two types of bHLH transcription factor determine the competence of the pericycle for lateral root initiation"}]},{"@id":"https://cir.nii.ac.jp/crid/1361694368214297344","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A stigmatic gene confers interspecies incompatibility in the Brassicaceae"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1186/s13059-015-0715-0"},{"@type":"CROSSREF","@value":"10.1007/978-1-4939-8657-6_17_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1016/j.ydbio.2017.01.022_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.3389/fgeed.2022.1085023_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1101/2020.06.30.179333_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1101/809368_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1038/s41477-018-0317-4_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1038/s41598-018-22667-1_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1093/pcp/pcw191_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.7554/elife.17023_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.7554/elife.60568_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1038/s41477-021-00919-9_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1111/nph.18724_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1186/s13007-021-00811-9_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1038/s41556-020-0515-y_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1093/pcp/pcac089_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1126/science.adp0779_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1038/srep26685_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1126/sciadv.aaz2963_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1093/pcp/pcab123_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1038/s41477-022-01176-0_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1111/nph.20277_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1126/science.aaz4131_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"},{"@type":"CROSSREF","@value":"10.1038/s41477-019-0444-6_references_DOI_VkIZSIGZEjR00l9vuQOnfkiV2uO"}]}