Cloning and Characterization of a Coronatine-Regulated Tyrosine Aminotransferase from Arabidopsis

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  • Anna Lopukhina
    Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität, D–44780 Bochum, Germany
  • Marcus Dettenberg
    Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität, D–44780 Bochum, Germany
  • Elmar W. Weiler
    Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität, D–44780 Bochum, Germany
  • Heike Holländer-Czytko
    Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität, D–44780 Bochum, Germany

Description

<jats:title>Abstract</jats:title> <jats:p>In plants, the phytotoxin coronatine, which is an analog of the octadecanoids 12-oxo-phytodienoic acid and/or jasmonic acid, gives rise to a number of physiological responses similar to those of octadecanoids. To further elucidate the physiological role of these compounds, the differential RNA display technique was used to isolate a number of novel octadecanoid-inducible genes expressed in coronatine-treated Arabidopsis. Among these, a cDNA clone was identified that was similar to known tyrosine aminotransferases (TATs). The function was verified with the expressed recombinant protein. In Arabidopsis, the protein is present as a multimer of 98 kD, with a monomer of an apparent molecular mass of 47 kD. TAT mRNA could be induced within 2 h by various octadecanoids and by wounding of the plants. Accumulation of the TAT protein and a 5- to 7-fold increase in its enzymatic activity was observed 7 to 9 h after application of octadecanoids, coronatine, or wounding. The potential role of TAT in the defense response to herbivores and pathogens is discussed.</jats:p>

Journal

  • Plant Physiology

    Plant Physiology 126 (4), 1678-1687, 2001-08-01

    Oxford University Press (OUP)

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