{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262944360428032.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/jb.179.11.3773-3782.1997"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/jb.179.11.3773-3782.1997"}}],"dc:title":[{"@value":"Escherichia coli endonuclease VIII: cloning, sequencing, and overexpression of the nei structural gene and characterization of nei and nei nth mutants"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Escherichia coli possesses two DNA glycosylase/apurinic lyase activities with overlapping substrate specificities, endonuclease III and endonuclease VIII, that recognize and remove oxidized pyrimidines from DNA. Endonuclease III is encoded by the nth gene. Endonuclease VIII has now been purified to apparent homogeneity, and the gene, nei, has been cloned by using reverse genetics. The gene nei is located at 16 min on the E. coli chromosome and encodes a 263-amino-acid protein which shows significant homology in the N-terminal and C-terminal regions to five bacterial Fpg proteins. A nei partial deletion replacement mutant was constructed, and deletion of nei was confirmed by genomic PCR, activity analysis, and Western blot analysis. nth nei double mutants were hypersensitive to ionizing radiation and hydrogen peroxide but not as sensitive as mutants devoid of base excision repair (xth nfo). Single nth mutants exhibited wild-type sensitivity to X rays, while nei mutants were consistently slightly more sensitive than the wild type. Double mutants lacking both endonucleases III and VIII exhibited a strong spontaneous mutator phenotype (about 20-fold) as determined by a rifampin forward mutation assay. In contrast to nth mutants, which showed a weak mutator phenotype, nei single mutants behaved as the wild type.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380580229801075725","@type":"Researcher","foaf:name":[{"@value":"D Jiang"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Molecular Genetics, The University of Vermont, Burlington 05405, USA."}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944360428032","@type":"Researcher","foaf:name":[{"@value":"Z Hatahet"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Molecular Genetics, The University of Vermont, Burlington 05405, USA."}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944360427904","@type":"Researcher","foaf:name":[{"@value":"J O Blaisdell"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Molecular Genetics, The University of Vermont, Burlington 05405, USA."}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944360427907","@type":"Researcher","foaf:name":[{"@value":"R J Melamede"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Molecular Genetics, The University of Vermont, Burlington 05405, USA."}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944360427906","@type":"Researcher","foaf:name":[{"@value":"S S Wallace"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Molecular Genetics, The University of Vermont, Burlington 05405, USA."}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00219193"},{"@type":"EISSN","@value":"10985530"},{"@type":"PISSN","@value":"https://id.crossref.org/issn/00219193"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/00219193"}],"prism:publicationName":[{"@value":"Journal of Bacteriology"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"1997-06","prism:volume":"179","prism:number":"11","prism:startingPage":"3773","prism:endingPage":"3782"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/jb.179.11.3773-3782.1997"}],"createdAt":"2016-11-10","modifiedAt":"2021-07-29","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050861770482354944","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The H2TH-like motif of the Escherichia coli multifunctional protein KsgA is required for DNA binding involved in DNA repair and the suppression of mutation frequencies"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565167558385408","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"KsgA, a 16S rRNA adenine methyltransferase, has a novel DNA glycosylase/AP lyase activity to prevent mutations in Escherichia coli"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679397362816","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"酸化ストレスにより生じるＤＮＡ損傷の修復機構"},{"@language":"en","@value":"Repair mechanism of DNA damage induced by oxidative stress"},{"@language":"ja-Kana","@value":"サンカ ストレス ニ ヨリ ショウジル DNA ソンショウ ノ シュウフク キコウ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679602525824","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Human DNA Glycosylases Involved in the Repair of Oxidatively Damaged DNA"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680192387072","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Purification and Characterization of a Novel DNA Repair Enzyme from the Extremely Radioresistant Bacterium <i>Rubrobacter radiotolerans</i>"},{"@value":"Purification and Characterization of a Novel DNA Repair Enzyme from the Extremely Radioresistant Bacterium Rubrobacter radiotolerans"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680234783744","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Development of Tester Strains Deficient in Nth/Nei DNA Glycosylases to Selectively Detect the Mutagenicity of Oxidized DNA Pyrimidines"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/jb.179.11.3773-3782.1997"},{"@type":"CROSSREF","@value":"10.1248/bpb.27.480_references_DOI_2qlfU0aIdAzq0ePfca85JPM3lsp"},{"@type":"CROSSREF","@value":"10.3123/jemsge.31.69_references_DOI_2qlfU0aIdAzq0ePfca85JPM3lsp"},{"@type":"CROSSREF","@value":"10.1093/nar/gkp057_references_DOI_2qlfU0aIdAzq0ePfca85JPM3lsp"},{"@type":"CROSSREF","@value":"10.1269/jrr.41.19_references_DOI_2qlfU0aIdAzq0ePfca85JPM3lsp"},{"@type":"CROSSREF","@value":"10.3123/jems.26.149_references_DOI_2qlfU0aIdAzq0ePfca85JPM3lsp"},{"@type":"CROSSREF","@value":"10.1186/s41021-023-00266-5_references_DOI_2qlfU0aIdAzq0ePfca85JPM3lsp"}]}