{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262944447078656.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/hep.25846"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fhep.25846"}},{"identifier":{"@type":"URI","@value":"https://journals.lww.com/01515467-201302000-00014"}}],"dc:title":[{"@value":"Microrna-27b Is a Regulatory Hub in Lipid Metabolism and Is Altered in Dyslipidemia"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:sec>\n            <jats:title/>\n            <jats:p>\n                                 <jats:bold>Cellular and plasma lipid levels are tightly controlled by complex gene regulatory mechanisms. Elevated plasma lipid content, or hyperlipidemia, is a significant risk factor for cardiovascular morbidity and mortality. MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression and have emerged as important modulators of lipid homeostasis, but the extent of their role has not been systematically investigated. In this study we performed high-throughput small RNA sequencing and detected ≈150 miRNAs in mouse liver. We then employed an unbiased,</jats:bold>\n                                 <jats:bold>\n                                    in silico\n                                 </jats:bold>\n                                 <jats:bold>strategy to identify miRNA regulatory hubs in lipid metabolism, and miR-27b was identified as the strongest such hub in human and mouse liver. In addition, hepatic miR-27b levels were determined to be sensitive to plasma hyperlipidemia, as evidenced by its ≈3-fold up-regulation in the liver of mice on a high-fat diet (42% calories from fat). Further, we showed in a human hepatocyte cell line (Huh7) that miR-27b regulates the expression (messenger RNA [mRNA] and protein) of several key lipid-metabolism genes, including</jats:bold>\n                                 <jats:bold>\n                                    Angptl3\n                                 </jats:bold>\n                                 <jats:bold>and</jats:bold>\n                                 <jats:bold>\n                                    Gpam\n                                 </jats:bold>\n                                 <jats:bold>. Finally, we demonstrated that hepatic miR-27b and its target genes are inversely altered in a mouse model of dyslipidemia and atherosclerosis.</jats:bold>\n                              </jats:p>\n          </jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion:</jats:title>\n            <jats:p>\n                                 <jats:bold>miR-27b is responsive to lipid levels and controls multiple genes critical to dyslipidemia.</jats:bold>\n                              </jats:p>\n          </jats:sec>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262944447078787","@type":"Researcher","foaf:name":[{"@value":"Kasey C. Vickers"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944447078784","@type":"Researcher","foaf:name":[{"@value":"Bassem M. Shoucri"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944447078790","@type":"Researcher","foaf:name":[{"@value":"Michael G. Levin"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944447078789","@type":"Researcher","foaf:name":[{"@value":"Han Wu"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944447078656","@type":"Researcher","foaf:name":[{"@value":"Daniel S. Pearson"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944447078657","@type":"Researcher","foaf:name":[{"@value":"David Osei-Hwedieh"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944447078785","@type":"Researcher","foaf:name":[{"@value":"Francis S. Collins"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944447078788","@type":"Researcher","foaf:name":[{"@value":"Alan T. Remaley"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944447078786","@type":"Researcher","foaf:name":[{"@value":"Praveen Sethupathy"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"02709139"}],"prism:publicationName":[{"@value":"Hepatology"}],"dc:publisher":[{"@value":"Ovid Technologies (Wolters Kluwer Health)"}],"prism:publicationDate":"2013-02","prism:volume":"57","prism:number":"2","prism:startingPage":"533","prism:endingPage":"542"},"reviewed":"false","dc:rights":["http://doi.wiley.com/10.1002/tdm_license_1.1"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fhep.25846"},{"@id":"https://journals.lww.com/01515467-201302000-00014"}],"createdAt":"2012-07-06","modifiedAt":"2024-12-01","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360285714407338368","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Zebrafish as a Model for Obesity and Diabetes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565170461630464","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Integrative genomic and proteomic analyses identifies glycerol-3-phosphate acyltransferase as a target of low-dose ionizing radiation in EBV infected-B cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182514415104","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"High-fat diet increases vulnerability to atrial arrhythmia by conduction disturbance via miR-27b"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846643399592320","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Transcriptomic Analysis of Chronic Hepatitis B and C and Liver Cancer Reveals MicroRNA-Mediated Control of Cholesterol Synthesis Programs"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680201436416","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"MicroRNAs and High-Density Lipoprotein Cholesterol Metabolism"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1002/hep.25846"},{"@type":"CROSSREF","@value":"10.1536/ihj.15-019_references_DOI_NGIgkX9QUsMMtQsgckuRG27Ax41"},{"@type":"CROSSREF","@value":"10.3389/fcell.2018.00091_references_DOI_NGIgkX9QUsMMtQsgckuRG27Ax41"},{"@type":"CROSSREF","@value":"10.3109/09553002.2015.1106021_references_DOI_NGIgkX9QUsMMtQsgckuRG27Ax41"},{"@type":"CROSSREF","@value":"10.1128/mbio.01500-15_references_DOI_NGIgkX9QUsMMtQsgckuRG27Ax41"},{"@type":"CROSSREF","@value":"10.1016/j.yjmcc.2015.11.034_references_DOI_NGIgkX9QUsMMtQsgckuRG27Ax41"}]}