{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262944555375616.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1007/s10856-010-4054-0"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/content/pdf/10.1007/s10856-010-4054-0.pdf"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/article/10.1007/s10856-010-4054-0/fulltext.html"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/content/pdf/10.1007/s10856-010-4054-0"}},{"identifier":{"@type":"PMID","@value":"20232232"}}],"dc:title":[{"@value":"Preparation and characterization of collagen microspheres for sustained release of VEGF"}],"description":[{"notation":[{"@value":"In this study, we prepared injectable collagen microspheres for the sustained delivery of recombinant human vascular endothelial growth factor (rhVEGF) for tissue engineering. Collagen solution was formed into microspheres under a water-in-oil emulsion condition, followed by crosslinking with water-soluble carbodiimide. Various sizes of collagen microspheres in the range of 1-30 mum diameters could be obtained by controlling the surfactant concentration and rotating speed of the emulsified mixture. Particle size proportionally decreased with increasing the rotating speed (1.8 mum per 100 rpm increase in the range of 300-1,200 rpm) and surfactant concentration (3.1 mum per 0.1% increase in the range of 0.1-0.5%). The collagen microspheres showed a slight positive charge of 8.86 and 3.15 mV in phosphate-buffered saline and culture medium, respectively. Release study showed the sustained release of rhVEGF for 4 weeks. Released rhVEGF was able to induce capillary formation of human umbilical vein endothelial cells, indicating the maintenance of rhVEGF bioactivity after release. In conclusion, the results suggest that the collagen microspheres have potential for sustained release of rhVEGF."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262944555375619","@type":"Researcher","foaf:name":[{"@value":"Nobuhiro Nagai"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944555375620","@type":"Researcher","foaf:name":[{"@value":"Norihiro Kumasaka"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944555375617","@type":"Researcher","foaf:name":[{"@value":"Takeaki Kawashima"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944555375616","@type":"Researcher","foaf:name":[{"@value":"Hirokazu Kaji"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944555375621","@type":"Researcher","foaf:name":[{"@value":"Matsuhiko Nishizawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944555375618","@type":"Researcher","foaf:name":[{"@value":"Toshiaki Abe"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09574530"},{"@type":"EISSN","@value":"15734838"}],"prism:publicationName":[{"@value":"Journal of Materials Science: Materials in Medicine"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2010-03-16","prism:volume":"21","prism:number":"6","prism:startingPage":"1891","prism:endingPage":"1898"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["http://www.springer.com/tdm"],"url":[{"@id":"http://link.springer.com/content/pdf/10.1007/s10856-010-4054-0.pdf"},{"@id":"http://link.springer.com/article/10.1007/s10856-010-4054-0/fulltext.html"},{"@id":"http://link.springer.com/content/pdf/10.1007/s10856-010-4054-0"}],"createdAt":"2010-03-15","modifiedAt":"2019-05-31","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Vascular%20Endothelial%20Growth%20Factor%20A","dc:title":"Vascular Endothelial Growth Factor A"},{"@id":"https://cir.nii.ac.jp/all?q=Umbilical%20Veins","dc:title":"Umbilical Veins"},{"@id":"https://cir.nii.ac.jp/all?q=Tissue%20Engineering","dc:title":"Tissue Engineering"},{"@id":"https://cir.nii.ac.jp/all?q=Vascular%20Endothelial%20Growth%20Factors","dc:title":"Vascular Endothelial Growth Factors"},{"@id":"https://cir.nii.ac.jp/all?q=Endothelial%20Cells","dc:title":"Endothelial Cells"},{"@id":"https://cir.nii.ac.jp/all?q=Biocompatible%20Materials","dc:title":"Biocompatible Materials"},{"@id":"https://cir.nii.ac.jp/all?q=Microspheres","dc:title":"Microspheres"},{"@id":"https://cir.nii.ac.jp/all?q=Emulsifying%20Agents","dc:title":"Emulsifying Agents"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Emulsions","dc:title":"Emulsions"},{"@id":"https://cir.nii.ac.jp/all?q=Collagen","dc:title":"Collagen"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360005519388801024","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Self-organization of hepatocyte morphogenesis depending on the size of collagen microbeads relative to hepatocytes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285706983282432","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A scalable controlled-release device for transscleral drug delivery to the retina"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848655088309632","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A self‐deploying drug release device using polymeric films"}]},{"@id":"https://cir.nii.ac.jp/crid/1361412891810437120","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Transscleral sustained ranibizumab delivery using an episcleral implantable device: Suppression of laser-induced choroidal neovascularization in rats"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1007/s10856-010-4054-0"},{"@type":"OPENAIRE","@value":"doi_dedup___::b3342a61fd4aed1198664c26eedb1f03"},{"@type":"CROSSREF","@value":"10.1088/1758-5090/ab145d_references_DOI_LdTzFeYuNoWzroXRH0GKeEMdfQs"},{"@type":"CROSSREF","@value":"10.1002/jbm.b.33887_references_DOI_LdTzFeYuNoWzroXRH0GKeEMdfQs"},{"@type":"CROSSREF","@value":"10.1016/j.ijpharm.2019.118458_references_DOI_LdTzFeYuNoWzroXRH0GKeEMdfQs"},{"@type":"CROSSREF","@value":"10.1016/j.biomaterials.2010.11.006_references_DOI_LdTzFeYuNoWzroXRH0GKeEMdfQs"}]}