{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262944568309632.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/jnr.21403"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjnr.21403"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/jnr.21403"}}],"dc:title":[{"@value":"Coherent anti‐stokes Raman scattering imaging of myelin degradation reveals a calcium‐dependent pathway in lyso‐PtdCho‐induced demyelination"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>Coherent anti‐Stokes Raman scattering (CARS) microscopy, which allows vibrational imaging of myelin sheath in its natural state, was applied to characterize lysophosphatidylcholine (lyso‐PtdCho)‐induced myelin degradation in tissues and in vivo. After the injection of lyso‐PtdCho into ex vivo spinal tissues or in vivo mouse sciatic nerves, myelin swelling characterized by the decrease of CARS intensity and loss of excitation polarization dependence was extensively observed. The swelling corresponds to myelin vesiculation and splitting observed by electron microscopy. The demyelination dynamics were quantified by the increase of g ratio measured from the CARS images. Treating spinal tissues with Ca<jats:sup>2+</jats:sup>ionophore A23187 resulted in the same kind of myelin degradation as lyso‐PtdCho. Moreover, the demyelination lesion size was significantly reduced upon preincubation of the spinal tissue with Ca<jats:sup>2+</jats:sup>free Krebs' solution or a cytosolic phospholipase A<jats:sub>2</jats:sub>(cPLA<jats:sub>2</jats:sub>) inhibitor or a calpain inhibitor. In accordance with the imaging results, removal of Ca<jats:sup>2+</jats:sup>or addition of cPLA<jats:sub>2</jats:sub>inhibitor or calpain inhibitor in the Krebs' solution remarkably increased the mean compound action potential amplitude in lyso‐PtdCho treated spinal tissues. Our results suggest that lyso‐PtdCho induces myelin degradation via Ca<jats:sup>2+</jats:sup>influx into myelin and subsequent activation of cPLA<jats:sub>2</jats:sub>and calpain, which break down the myelin lipids and proteins. The current work also shows that CARS microscopy is a potentially powerful tool for the study of demyelination. © 2007 Wiley‐Liss, Inc.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262944568309633","@type":"Researcher","foaf:name":[{"@value":"Yan Fu"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944568309634","@type":"Researcher","foaf:name":[{"@value":"Haifeng Wang"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944568309632","@type":"Researcher","foaf:name":[{"@value":"Terry B. Huff"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944568309636","@type":"Researcher","foaf:name":[{"@value":"Riyi Shi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380016867743928832","@type":"Researcher","foaf:name":[{"@value":"Ji‐Xin Cheng"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03604012"},{"@type":"EISSN","@value":"10974547"}],"prism:publicationName":[{"@value":"Journal of Neuroscience Research"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2007-06-05","prism:volume":"85","prism:number":"13","prism:startingPage":"2870","prism:endingPage":"2881"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjnr.21403"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/jnr.21403"}],"createdAt":"2007-06-06","modifiedAt":"2025-01-17","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360285706210760832","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Label-free detection of peripheral nerve tissues against adjacent tissues by spontaneous Raman microspectroscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360576118818804480","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Macroscopic detection of demyelinated lesions in mouse PNS with neutral red dye"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204141476992","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Molecular Spectroscopic Imaging Using a White-Light Laser Source"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1002/jnr.21403"},{"@type":"CROSSREF","@value":"10.1007/s00418-012-1015-3_references_DOI_HQKXqcPMIIozf6kqmOBjgROH2yz"},{"@type":"CROSSREF","@value":"10.1246/bcsj.20100004_references_DOI_HQKXqcPMIIozf6kqmOBjgROH2yz"},{"@type":"CROSSREF","@value":"10.1038/s41598-021-96395-4_references_DOI_HQKXqcPMIIozf6kqmOBjgROH2yz"}]}